The role of neutrophil chemotactic cytokines in the pathogenesis of equine chronic obstructive pulmonary disease (COPD)

Franchini, Marco; Gilli, Urs O.; Akens, M. K.; Fellenberg, R. von; Bracher, Verena

doi:10.1016/s0165-2427(98)00178-0

Cited by 75 publications

(53 citation statements)

References 11 publications

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“…IL-8 is a potent neutrophil chemoattractant in human inflammatory airway diseases (32), and this has also been demonstrated for RAO-affected horses (12). Indeed, in RAO-affected horses there was a significant correlation between the amounts of epithelial cell-derived IL-8 mRNA and neutrophils in BAL (Fig.…”

Section: Discussionsupporting

confidence: 57%

Elevated amount of Toll-like receptor 4 mRNA in bronchial epithelial cells is associated with airway inflammation in horses with recurrent airway obstruction

Berndt¹,

Derksen²,

Venta³

et al. 2007

American Journal of Physiology-Lung Cellular and Molecular Phys

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Section: Discussionsupporting

confidence: 57%

Elevated amount of Toll-like receptor 4 mRNA in bronchial epithelial cells is associated with airway inflammation in horses with recurrent airway obstruction

Berndt¹,

Derksen²,

Venta³

et al. 2007

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…13 Researchers in another study failed to show a significant increase in chemotactic activity in BALF from RAO-affected horses compared with healthy horses, and the chemotactic activity did not correlate with the percentage of neutrophils in BALF. 11 However, in that study, RAO-affected horses exposed to dust had significantly higher chemotactic activity than did RAO-affected horses with low dust exposure. In vitro stimulation of BALF cells obtained from an RAOaffected horse in clinical remission showed an increase in mRNA specific for IL-8 and MIP-2, which was associated with an increase in chemotactic activity in the supernatant.…”

Section: Discussionmentioning

confidence: 59%

“…24 Researchers have shown that in vitro stimulation of equine bronchoalveolar macrophages results in the induction of expression of the chemokines interleukin (IL)-8 and macrophage inflammatory protein (MIP)-2, both of which are potent neutrophil chemoattractants. 11 Concentrations of IL-8 have also been shown to be increased in bronchoalveolar lavage fluid (BALF) of horses with RAO after exposure to hay. 12 Airway epithelial cells might also produce cytokines (e.g., IL-8) that result in neutrophil migration into airways and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Rapid, Multiwell Colorimetric Assay for Measuring Neutrophil Chemoattractant Activity in Bronchoalveolar Lavage Fluid of Horses with Recurrent Airway Obstruction

et al. 2006

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Abstract. The criteria used to diagnose recurrent airway obstruction (RAO) in affected horses include demonstration of reversible lower airway obstruction and greater than 25% neutrophils in bronchoalveolar lavage fluid (BALF). Additional objective laboratory tests are needed to improve diagnostic accuracy and to monitor response to treatment. The goal of this study was to determine if neutrophil chemoattractant activity of BALF could be measured by using a previously described, rapid, multiwell colorimetric assay for chemotaxis. In this assay, neutrophils that have migrated through a membrane filter are collected into the bottom well of a disposable chemotaxis-cell migration chamber. The number of viable cells collected in the bottom well is quantified by measurement of the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenol tetrazolium bromide (MTT), which is reduced by dehydrogenase in mitochondria of live cells. The number of migrating cells corresponds to the amount of MTT reduced, which is measured with an enzyme-linked immunosorbent assay plate reader. Fourteen adult horses were enrolled in this study, 7 of which had owner histories consistent with RAO. Each horse was sedated, a bronchoalveolar lavage tube was passed, and saline was infused and immediately aspirated. An aliquot of BALF was used for differential cell count, and BALF supernatant was harvested to assess neutrophil chemoattractant activity. Normal control horses and RAOaffected horses were distinguished according to clinical signs and percent neutrophils in BALF. Neutrophil chemoattractant activity of BALF was significantly greater in RAO-affected horses (P 5 0.001) compared with control horses. This assay may be useful in future studies for monitoring response to therapy in RAOaffected horses.

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“…The synthesis of this factor was also upregulated in the airway secretions obtained from humans with asthma and horses with heaves (Franchini et al 1998, Ainsworth et al 2003, Tsoumakidou et al 2004. It has been hypothesized that a higher level of IFN-γ may be a marker of an ongoing inflammatory process which is more pronounced in patients with a chronic form of the disease.…”

Section: Discussionmentioning

confidence: 98%

Evaluation of serum cytokine levels in recurrent airway obstruction

Niedźwiedź¹,

Borowicz²,

Kubiak³

et al. 2016

Polish Journal of Veterinary Sciences

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Recurrent airway obstruction (RAO) represents a serious health problem and is traditionally classified as an allergic disease, where contact with an antigen can induce clinical airway inflammation, bronchial hyper-responsiveness and reversible airway obstruction. Previous studies have demonstrated the presence of the Th2 response in the lungs of human patients with asthma and horses with heaves. These cells are involved in the production of cytokines which regulate the synthesis of immunoglobulins. 40 horses were evaluated: 30 horses with RAO and 10 healthy animals. The expression levels of interferon-alpha 1 (IFN-α 1 ), interferon-gamma (IFN-γ), interleukin-1β, (IL-1β), IL-2, IL-4, IL-13 and tumor necrosis factor alpha (TNF-α) were measured in the serum obtained from control and RAO-susceptible horses during crisis. In all the patients, serum cytokine levels were detected. Serum median IL-13 and IFN-γ levels were significantly higher in RAO-affected horses than in the healthy group (p < 0.001). The serum median IFN-α 1 , IL-1β, IL-2, IL-4, and TNF-α levels were similar in both groups. These results indicate a low variability of the levels of cytokines and a high frequency of their detection in serum samples from horses with RAO. Immune mechanisms involved in equine RAO are more complex than those defined by a simple Th1/Th2 dichotomy.

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The role of neutrophil chemotactic cytokines in the pathogenesis of equine chronic obstructive pulmonary disease (COPD)

Cited by 75 publications

References 11 publications

Elevated amount of Toll-like receptor 4 mRNA in bronchial epithelial cells is associated with airway inflammation in horses with recurrent airway obstruction

Elevated amount of Toll-like receptor 4 mRNA in bronchial epithelial cells is associated with airway inflammation in horses with recurrent airway obstruction

Rapid, Multiwell Colorimetric Assay for Measuring Neutrophil Chemoattractant Activity in Bronchoalveolar Lavage Fluid of Horses with Recurrent Airway Obstruction

Evaluation of serum cytokine levels in recurrent airway obstruction

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