The role of OX40L and ICAM-1 in the stability of coronary atherosclerotic plaques and their relationship with sudden coronary death

Abstract

BackgroundCoronary heart disease is related to sudden death caused by multi-factors and a major threat to human health.This study explores the role of OX40L and ICAM-1 in the stability of coronary plaques and their relationship with sudden coronary death.MethodsA total of 118 human coronary arteries with different degrees of atherosclerosis and/or sudden coronary death comprised the experimental group and 28 healthy subjects constituted the control group were isolated from patients. The experimental group was subdivided based on whether the cause of death was sudden coronary death and whether it was accompanied by thrombosis, plaque rupture, plaque outflow and other secondary changes: group I: patients with coronary atherosclerosis but not sudden coronary death, group II: sudden coronary death without any of the secondary changes mentioned above, group III: sudden coronary death with coronary artery atherosclerotic lesions accompanied by either of the above secondary changes. The histological structure of the coronary artery was observed under a light microscope after routine HE staining, and the related indexes of atherosclerotic plaque lesions were assessed by image analysis software. The expressions of OX40L and ICAM-1 were detected by real-time quantitative PCR (RT-PCR), immunohistochemistry (IHC) and Western blotting, and the correlations between the expressions and the stability of coronary atherosclerotic plaque and sudden coronary death were analyzed.Results(1) The expression of OX40L protein in the control group and the three experimental groups showed an increasing trend, and the difference between groups was statistically significant (P < 0.05). (2) The expression of the ICAM-1 protein in the control group and the three experimental groups showed a statistically significant (P < 0.05) increasing trend. (3) The expression of OX40L and ICAM-1 mRNAs increased in the control and the three experimental groups and the difference was statistically significant (P < 0.05).ConclusionThe expression of OX40L and ICAM-1 proteins and mRNAs is positively correlated with the stability of coronary atherosclerotic plaque and sudden coronary death.

PRDX2 Protects Against Atherosclerosis by Regulating the Phenotype and Function of the Vascular Smooth Muscle Cell

“…The VCAM-1 increased in VSMCs with treatment of TNF-α, which play a key role in AS (32). ICAM-1, a member of the immunoglobulin superfamily, is widely expressed in various cells under the pathological condition, enhancing the adhesion between cells and vascular endothelium (33)(34)(35). The expression of adhesion molecules in lesions can affect the tissue of the cells, promote the production of cytokines and growth factors and the migration of medial VSMCs to the inner membrane, and affect the replication of the cells (36).…”

Research on the correlation between activating transcription factor 3 expression in the human coronary artery and atherosclerotic plaque stability

“…The coronary artery tissue in all cases were fixed with 4% paraformaldehyde for 72 h. The cross section of the blood vessel was embedded in paraffin, 4-μm sections were prepared and stained with conventional HE, and an EasyScan digital section scanning and application system (McAudi Industrial Group Co., Ltd., China) was used to observe the morphology and structure of the coronary artery. IPP 6.0 image analysis software was used to detect the related morphological indexes[ 13 ]: (1) intimal and lesion thicknesses: From the free edge of the endocardial cavity surface to the vertical distance of the internal elastic membrane, the thickest and thinnest thickness of the intima was measured, and another straight line was perpendicular to the two test lines. The mean of the test line and the average thickness of the intima were calculated.…”

The predictive value of lymphocyte-to-monocyte ratio in the prognosis of acute coronary syndrome patients: a systematic review and meta-analysis

“…Previous studies indicate that approximately half of deaths from CHD occur after ACS [ 3 , 4 ]. Rupture of atherosclerotic plaques and formation of thrombi are the main cause of ACS [ 5 – 7 ]. The atherosclerotic plaques are associated with the infiltration of inflammatory cells (lymphocytes, monocytes, and neutrophils) [ 8 – 10 ].…”