The role of p53, bax, bcl2, and 8-OHdG in human acute myocardial infarction

Abstract

Apoptosis is implicated in unfavorable remodeling of the left ventricle during acute myocardial infarction (AMI). Both DNA damage and p53 play important roles in regulating apoptosis. Expression patterns of apoptotic regulating genes such as p53, bax, and bcl-2 highlight the importance of inhibiting ventricle remodeling and subsequent injuries. In the present study, serum levels of p53 and 8-hydroxy-2-deoxyguanosine (8-OHdG) as well as p53, bax, and bcl-2 expression were examined after the onset of AMI in Iranian patients. Serum levels of p53 and 8-OHdG were measured by enzyme-linked immunosorbent assay (ELISA) and the presence of p53 protein and mRNA expression of p53, bax, and bcl-2 were analyzed by Western blotting and real time RT-PCR methods respectively. In patients presenting with AMI, serum levels of p53 and 8-OHdG were increased in comparison to healthy controls. Likewise, transcripts of p53 and bax were also elevated in patients while bcl-2 was decreased. Collectively, our data suggest the novel use of p53 and 8-OHdG as markers of apoptosis and DNA damage following AMI. Our results also revealed that apoptosis occurs in concert with an up-regulation of p53 and bax and a down-regulation of bcl-2 which may suggest a possible therapeutic intervention in patients recovering from AMI.© Versita Sp. z o.o.

8-Hydroxy-2-Deoxyguanosine Levels and Cardiovascular Disease: A Systematic Review and Meta-Analysis of the Literature

“…Thus, 14 studies ( 2 , 3 , 5 , 13 , 15 , 16 , 21 , 24 , 25 , 28 , 30 , 35 , 37 , 46 ) on 810 CVD patients and 1106 controls were included in the final analysis ( Supplementary Fig. S1 ).…”

“…S1 ). In detail, three studies on stroke (245 cases and 566 controls) ( 5 , 24 , 37 ), seven on CAD (351 cases and 404 controls) ( 2 , 3 , 13 , 21 , 28 , 30 , 46 ), two on patients with carotid atherosclerosis undergoing carotid endarterectomy (134 cases and 66 controls) ( 16 , 35 ), and two on peripheral artery disease (80 cases and 70 controls) ( 15 , 25 ) were included.…”

Prognostic Value of Pentraxin-3 Level in Patients with STEMI and Its Relationship with Heart Failure and Markers of Oxidative Stress

“…In our study we found a weak significant correlation between levels of 8-OHdG upon hospital admission and PTX3 concentration 24 h after MI onset. Previously reported studies have shown that increased serum levels of 8-OHdG in patients with MI correspond with the severity of heart failure and left ventricular dysfunction [ 37 , 38 ]. On the other hand, in case of MDA, another marker of oxidative stress, no significant correlation with levels of PTX3 was found.…”

Brahma-related gene 1 induces apoptosis in a p53-dependent manner in human rheumatoid fibroblast-like synoviocyte MH7A

“…Previous reports have shown that p53 is up-regulated while BCL2 is down-regulated to induce CYCS release from the mitochondrion, which further activates CASP9 and CASP3 to encourage cell apoptosis. [29,30] The regulation of these factors by p53 may be directly or indirectly dependent on the transcription-regulatory function of p53. [31,32] The modulated apoptotic factors by BRG1 and the BRG1-induced MH7A apoptosis found in this study imply one possible mechanism of BRG1 in regulating MH7A apoptosis.…”