The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide

Abstract

Background The present study investigated the effect of thiamine disulfide (TD) on the pancreas in terms of hyperglycemia improvement and insulin sensitivity increase in diabetic male rats. We also aimed to study the function of Pdx1 (pancreatic and duodenal homeobox 1) and Glut2 (glucose transporter 2) genes in pancreatic tissue. Methods Type 1 diabetes was induced through injection of 60 mg/kg streptozotocin (STZ). The diabetic rats were divided into four groups, namely diabetic control (DC), diabetic treated with thiamine disulfide (D-TD), diabetic treated with insulin (D-insulin), and diabetic treated with TD and insulin (D-insulin+TD). The non-diabetic (NDC) and diabetic groups received a normal diet (14 weeks). Blood glucose level and body weight were measured weekly; insulin tolerance test (ITT) and glucagon tolerance test (GTT) were performed in the last month of the study. The level of serum insulin and glucagon were measured monthly and a hyperglycemic clamp (Insulin Infusion rate (IIR)) was done for all the groups. Pancreas tissue was isolated so that Pdx1and Glut2 genes expression could be measured. Results We observed that TD therapy decreased blood glucose level, ITT, and serum glucagon levels in comparison with those of the DC group; it also increased serum insulin levels, IIR, and expression of Pdx1 and Glut2 genes in comparison with those of the DC group. Conclusion Administration of TD could improve hyperglycemia in type 1 diabetic animals through improved pancreas function. Therefore, not only does TD have a significant effect on controlling and reducing hyperglycemia in diabetes, but it also has the potential to decrease the dose of insulin administration.

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Applying Network Pharmacology and Molecular Docking in the Screening for Molecular Mechanisms of Ampalaya (Momordica charantia L.) and Banaba (Lagerstroemia speciosa L.) against Type 2 Diabetes Mellitus

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Dapagliflozin Mitigates Hypotension in Lipopolysaccharide-Induced Acute Inflammation Independent of Glycemia Level

“…Twenty-four rats were treated with a single dose of streptozotocin (STZ) (Sigma-Aldrich, St. Louis, MO, USA) (60 mg/kg, i.p.) freshly dissolved in 0.1 mM sodium citrate buffer adjusted to pH 4.5 [ 27 ]. After injection, a 5% sucrose solution was provided for 48 h to avoid hypoglycemic shock.…”

“…In addition, twenty-four rats were treated with a single dose of 0.1 mM sodium citrate buffer as a vehicle for the non-diabetic groups. Blood was obtained from the rat tail vein on the fifth day to measure fasting blood glucose (FBG) levels using a glucometer (ACCU-Chek Performa, Basel, Switzerland) to confirm each rat’s diabetic stage (250 mg/dL) [ 27 ].…”