The role of phosphoinositiees in signal transduction

Sekar, Mahendran; Hokin, Lowell E.

doi:10.1007/bf01870664

Cited by 226 publications

(74 citation statements)

References 323 publications

(137 reference statements)

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“…A variety of cell types involved is known to have extracellular specific receptors to AA metabolites [9,10]. PLA2, a catalyzer of the hydrolysis of choline and ethanolamine glycerophospholipid, is the key enzyme responsible for AA release at different signal reactions in mammalian cells [11][12][13]. The released AA is consequently oxidized either by cyclooxygenase with formation of cyclic products like prostaglandins, or by lipoxygenase giving rise to linear chain products such as lipoxines and leukotrienes [8,13].…”

Section: Introductionmentioning

confidence: 99%

Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation

et al. 1996

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The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [3H]arachidonic acid-labeled thymocytes release radioactive products to the external medium. The process is blocked by the lipoxygenase inhibitor, nordihydroguaiaretic acid. Thus, it can be concluded that irradiation activates arachidonic acid metabolism and that lipoxygenase metabolites play an important role in thymocyte apoptosis.

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Section: Introductionmentioning

confidence: 99%

Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation

et al. 1996

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“…The inverse hexagonal phase was observed over a wide temperature and pressure range at physiological pH and hydration. The evidence from this study is striking because 2 mol % is a typical physiological concentration for this lipid [3,19]. There is now evidence that a physiological concentration of PI-4-P is required for the flippase activity of Drs2p on PS.…”

mentioning

confidence: 58%

“…1). PIP2, along with phosphatidylinositol (PI) and phosphatidylinositol 4-phosphate (PI-4-P) make up the bulk of inositide lipids in vivo [2,3]. The biological importance of PIP2 and PIP3 is exemplified by the effect of mutations in the proteins that convert the former into the latter.…”

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confidence: 99%

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The physical influence of inositides—a disproportionate effect?

Furse

2014

J Chem Biol

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After the initial observation that lipids form a considerable part of biological membranes, the details of the physical role of lipids in biological systems have emerged gradually. There have been few 'Eureka' moments in which a class or individual lipid has appeared as a game-changing physical player. However, evidence collected in the last five years suggests that that notion may be about to change. In chemical biology studies, inositides are increasingly showing themselves to be lipids that have a physical influence on membrane systems that is as strong as their biological (signalling) one. Additionally, recent evidence has shown that the concentration of at least one inositide changes during important stages of the cell cycle, and not in a manner consistent with its traditional signalling roles. The balance between these data is explored and a forward-looking view is proposed. The apparent ubiquity of these two inositides in mammalian systems has aroused research interest from several angles. There is a mounting body of evidence that it is not only mammals that make use of PIP2 as a signalling molecule, but also insects [9] and plants [10]. Several studies have suggested that PIP2 has a physical role that might be quite separate from its biological one. The particular processes in which PIP2 is a protagonist include membrane trafficking [11,12], vesicle formation [12,13], clatharin-mediated endocytosis [13,14], and in membrane dynamics [15]. There is also some indication that the activity of phospholipase Cγ, for which PIP2 is a substrate, may have a physical role in membrane fusion [16]. It also seems likely that PIP2 is a team player, acting with other lipids such that certain physical processes may be completed. A study of pollen tubes in Arabidopsis provided evidence that a particular balance between PIP2 and PI-4-P is required for endocytosis to be successful [17]. There have not yet been any comprehensive studies of the phase behaviour of PIP2, and thus, it is not yet clear whether it is typically a bilayer-forming lipid or one that has an energetic preference for a curved topology.Despite the paucity of phase behaviour data on PIP2, the phase behaviour of the physiological precursor to PIP2, PI-4-P, has been researched. Furse et al. reported that PI-4-P is able to induce an inverse hexagonal phase in the typically-lamellar PC, at 2 mol % [18], strongly suggesting that PI-4-P is not a

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“…OAG, I-oleoyl-2-acetylglycerol; dbcAMP, dibutyryl cyclic AMP; PS, phosphatidylserine quite general mechanism for regulation in mammalian [4] and plant cells [5]. Diacylglycerol (DG), one of the two second messengers generated by such a mechanism, causes activation of a Ca'+/phospholipid-dependent kinase, called protein kinase C (PKC) [6].…”

Section: Mexicomentioning

confidence: 99%

12‐O‐Tetradecanoyl phorbol‐13‐acetate interferes with germination of Phycomyces blakesleeanus sporangiospores

1988

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The presence of protein kinase C (PKC), a key enzyme in signal transduction, has not been investigated in fungal cells. The phorbol ester TPA, an activator of PKC, may be used as an indicator of the presence and role of PKC in Phycomyces blakesleeanus spores. Activation of spore germination by acetate was prevented by 6 nM TPA. The TPA analog 4aPDD, an ineffective activator of PKC, did not affect spore germination. 3 mM dbcAMP, on the other hand, reversed the inhibition of germination caused by TPA. TPA-stimulated protein kinase activity was detected in spores. The possible relationship between PKC and the increased levels of CAMP that accompany the induction of spore germination is discussed.

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The role of phosphoinositiees in signal transduction

Cited by 226 publications

References 323 publications

Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation

Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation

The physical influence of inositides—a disproportionate effect?

12‐O‐Tetradecanoyl phorbol‐13‐acetate interferes with germination of Phycomyces blakesleeanus sporangiospores

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