The safety of water supply network has always been a concern. The
biofilm attached on the pipes will fall into pipe-water under the
disturbance, which becomes a potential threat towards human health.
Particulate matter can provide shelter for bacteria, and most bacteria
in water will adhere to the surface of it, which is not easy to be
killed by disinfectants. Therefore, the effect caused by particulate
matters and extracellular polymer (EPS) on the stability of biofilm was
investigated. This study revealed that the biofilms without particulate
matters showed the highest biomass, that’s an order of magnitude more
than have particulate matter bacteria, while those with abundant
particulate matters and bacteria were more stable. The effect of shear
stress towards the biofilm attached on the pipes was investigated
through rotational speed experiments, and the results implied that the
collision between bacterial polymers increased with the increasing of
rotational speed, simultaneously with the formation of stale biofilm
adhered inside the pipes. Besides, instead of protein and fatty chains,
the hydroxyl bonds played vital roles during the combination of
particulate matters and EPS.