The Role of Polyphosphates in Sugar Transport Across the Plasma Membrane of Yeast

Steveninck, J. Van; Tijssen, J.P.F.; Dubbelman, T. M. A. R.; Broek, Peter J.A. Van den

doi:10.1016/b978-0-12-428580-4.50043-5

Cited by 3 publications

(4 citation statements)

References 18 publications

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“…* 1, High-affinity carrier; 2, medium-affinity carrier; 3, low-affinity carrier. Franzusoff & Cirillo, 1982;van Steveninck et al, 1985). The possibility that the observed changes in the kinetics of glucose transport may be explained by changes in hexokinase activities was investigated (Fig.…”

Section: Glutamate-limited Cultures At Diflerent Dilution Rates In Comentioning

confidence: 99%

“…Glucose can be transported into yeasts via three different uptake mechanisms : by facilitated diffusion (Romano, 1982); by active transport through a proton-glucose symporter as shown for species of Candida (Spencer-Martins & van Uden 1985a) and Rhodosporidium (Hauer & Hofer, 1982;Hofer & Misra, 1978); or by a group translocation process in which the glucose is phosphorylated during transport (van Steveninck et al, 1985). In a variety of cases the dependence of the rate of transport on the assay sugar concentration yielded a biphasic kinetic plot indicating the presence of two uptake systems (Bisson & Fraenkel, 1983 ;Spencer-Martins & van Uden, 1985a;van den Broek et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Adaptation of the Kinetics of Glucose Transport to Environmental Conditions in the Yeast Candida utilis CBS 621: a Continuous-culture Study

Postma¹,

Scheffers²,

Dijken³

1988

Microbiology

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~~~ ~The relation between the kinetic parameters of glucose transport and the physiology of Candida utilis CBS 621 was studied in chemostat cultures. In glucose-limited cultures the transport parameters were dependent on the growth rate of the yeast. Three different transport systems were found which differed by an order of magnitude in their affinity constants, namely a highaffinity ( K , 25 p~), a medium-affinity ( K , 190 p~), and a low-affinity uptake system ( K , 2000 p~). Cells growing at a dilution rate of 0.45 h-l or less had the high-and medium-affinity uptake systems. At a dilution rate of 0.52 h-I the high-affinity system was absent and both the mediumand low-affinity systems were present. At a dilution rate close to pmax (0.57 h-l) only the lowaffinity system was detected. The in situ contribution of each of the transport systems to glucose consumption in glucose-limited cultures was estimated on the basis of their kinetic parameters ( K , and V,,,,,) and the residual glucose concentration in these cultures. The sum of the calculated rates of transport corresponded to the in situ rate of glucose consumption by the cultures as determined from the yield constant and the dilution rate. The dependence of the transport parameters on the growth rate and hence on the environmental sugar concentration was also evident in cells grown under nitrogen limitation. In contrast to carbon-limited cells, nitrogenlimited cultures growing at D = 0.15 h-' did not exhibit the high-affinity glucose uptake system, whereas the medium-and low-affinity systems were present.

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Section: Glutamate-limited Cultures At Diflerent Dilution Rates In Comentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adaptation of the Kinetics of Glucose Transport to Environmental Conditions in the Yeast Candida utilis CBS 621: a Continuous-culture Study

Postma¹,

Scheffers²,

Dijken³

1988

Microbiology

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“…Also a phosphate-glucose group translocation mechanism has been suggested (Van Steveninck et al, 1985;Franzusoff & Cirillo, 1982). Bisson & Fraenkel(l983) and Lang & Cirillo (1987) reported the presence of a so-called high-affinity transporter (K, 1 mM).…”

Section: Introductionmentioning

confidence: 99%

Glucose Transport in Crabtree-positive and Crabtree-negative Yeasts

et al. 1989

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The kinetic parameters of glucose transport in four Crabtree-positive and four Crabtreenegative yeasts were determined. The organisms were grown in aerobic glucose-limited chemostats at a dilution rate of 0.1 h-l . The results show a clear correlation between the presence of high-affinity glucose transport systems and the absence of aerobic fermentation upon addition of excess glucose to steady-state cultures. The presence of these H+-symport systems could be established by determination of intracellular accumulation of 6-deo~y-[~H]glucose and alkalinization of buffered cell suspensions upon addition of glucose. In contrast, the yeasts that did show aerobic alcoholic fermentation during these glucose pulse experiments had low-affinity facilitated-diffusion carriers only. In the yeasts examined the capacity of the glucose transport carriers was higher than the actual glucose consumption rates during the glucose pulse experiments. The relationship between the rate of sugar consumption and the rate of alcoholic fermentation was studied in detail with Saccharomyces cereuisiae. When S . cerevisiae was pulsed with low amounts of glucose or mannose, in order to obtain submaximal sugar consumption rates, fermentation was already occurring at sugar consumption rates just above those which were maintained in the glucose-limited steady-state culture. The results are interpreted in relation with the Crabtree effect. In Crabtree-positive yeasts, an increase in the external glucose concentration may lead to unrestricted glucose uptake by facilitated diffusion and hence, to aerobic fermentation. In contrast, Crabtree-negative yeasts may restrict the entry of glucose by their regulated H+-symport systems and thus prevent the occurrence of overflow metabolism.

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“…For the other glucose carrier, which has a n affinity constant of 1-5 mM, confusing data exist in the literature. Some authors have proposed a phosphotransferase model with involvement of hexokinases, and possibly of polyphosphate during the transport process (Franzusoff and Cirillo, 1982;Bisson and Fraenkel, 1983;van Steveninck et al, 1985), whereas others have proposed a facilitated-diffusion mechanism (Romano, 1982).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of growth and glucose transport in glucose‐limited chemostat cultures of Saccharomyces cerevisiae CBS 8066

Postma

Scheffers

Dijken

1989

Yeast

110

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The glucose transport capacity of Saccharomyces cerevisiae CBS 8066 was studied in aerobic glucose-limited chemostat cultures. Two different transport systems were encountered with affinity constants of 1 and 20 mM, respectively. The capacity of these carriers (Vmax) was dependent on the dilution rate and the residual glucose concentration in the culture. From the residual glucose concentration in the fermenter and the kinetic constants of glucose transport, their in situ contribution to glucose consumption was determined. The sum of these calculated in situ transport rates correlated well with the observed rate of glucose consumption of the culture. The growth kinetics of S. cerevisiae CBS 8066 in glucose-limited cultures were rather peculiar. At low dilution rates, at which glucose was completely respired, the glucose concentration in the fermenter was constant at 110 microM, independent of the glucose concentration in the reservoir. At higher dilution rates, characterized by the occurrence of both respiration and alcoholic fermentation, the residual substrate concentration followed Monod kinetics. In this case, however, the overall affinity constant was dependent on the reservoir glucose concentration.

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The Role of Polyphosphates in Sugar Transport Across the Plasma Membrane of Yeast

Cited by 3 publications

References 18 publications

Adaptation of the Kinetics of Glucose Transport to Environmental Conditions in the Yeast Candida utilis CBS 621: a Continuous-culture Study

Adaptation of the Kinetics of Glucose Transport to Environmental Conditions in the Yeast Candida utilis CBS 621: a Continuous-culture Study

Glucose Transport in Crabtree-positive and Crabtree-negative Yeasts

Kinetics of growth and glucose transport in glucose‐limited chemostat cultures of Saccharomyces cerevisiae CBS 8066

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