The role of protein hydrolysates for exercise-induced skeletal muscle recovery and adaptation: a current perspective

Morgan, Paul T.; Breen, Leigh

doi:10.1186/s12986-021-00574-z

Cited by 29 publications

(26 citation statements)

References 175 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The smaller size peptides exhibited higher bioactivities, especially cell proliferation activity [Chotphruethipong et al, 2021c,d]. Also, short chain peptides could be rapidly digested and absorbed in the human body [Morgan & Breen, 2021].…”

Section: Resultsmentioning

confidence: 99%

Hydrolyzed Collagen from Salmon Skin Increases the Migration and Filopodia Formation of Skin Keratinocytes by Activation of FAK/Src Pathway

Woonnoi¹,

Chotphruethipong²,

Tanasawet³

et al. 2021

Pol. J. Food Nutr. Sci.

View full text Add to dashboard Cite

Previous studies reported hydrolyzed collagen increase cell proliferation and migration involved in the wound repair process. Nevertheless, the knowledge related with wound repair mechanism of hydrolyzed collagen from salmon skin (HCSS) has not been fully elucidated. Therefore, this study aimed to elucidate the effects of HCSS on the migration of keratinocyte HaCaT cells. Additionally, its molecular mechanism through cell division control protein 42 (Cdc42), Ras-related C3 botulinum toxin substrate 1 (Rac1), and Ras homolog family member A (RhoA) via focal adhesion kinase (FAK)-steroid receptor coactivator (Src) regulation and keratinocyte stem cells (KSCs) markers were also evaluated. After 24 h of incubation, keratinocyte proliferation was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and double stranded DNA (dsDNA) assays, and by determining the total cellular protein content. Keratinocyte migration and filopodia formation were measured by wound healing assay and phalloidin-rhodamine staining, respectively. The migratory related proteins were evaluated by western blot analysis. HCSS had a high content of hydrophobic amino acids and imino acids. HaCaT cell proliferation and migration were significantly increased in response to HCSS at the concentration of 100-1000 μg/mL. The formation of filopodia was subsequently increased in response to HCSS at concentrations of 100-1000 μg/mL. Moreover, HCSS upregulated Cdc42, Rac1, and RhoA protein expression and activated the phosphorylation of FAK and Src pathway. HCSS at the concentration of 100-1000 μg/mL could trigger stemness by increased KSC markers, including keratin 19 and β-catenin expression. This study has demonstrated that HCSS induces proliferation and migration of keratinocytes, subsequently promotes the second phase of wound healing process by FAK-Src activation and also increases the KSC properties.

show abstract

Section: Resultsmentioning

confidence: 99%

Hydrolyzed Collagen from Salmon Skin Increases the Migration and Filopodia Formation of Skin Keratinocytes by Activation of FAK/Src Pathway

Woonnoi¹,

Chotphruethipong²,

Tanasawet³

et al. 2021

Pol. J. Food Nutr. Sci.

View full text Add to dashboard Cite

show abstract

“…Apparently, this hyper aminoacidemia also seems to be related to a possible thermogenic effect, which could have beneficial effects in athletes to reduce their fat body mass. Although further research is needed to confirm all these effects (85,86). Other beneficial effects of protein hydrolysates on sport performance could be related to the generation of bioactive peptides.…”

Section: Hydrolysates and Peptidesmentioning

confidence: 99%

Protein and Sport: Alternative Sources and Strategies for Bioactive and Sustainable Sports Nutrition

2022

View full text Add to dashboard Cite

Nutrition and sport play an important role in achieving a healthy lifestyle. In addition to the intake of nutrients derived from the normal diet, some sport disciplines require the consumption of supplements that contribute positively to improved athletic performance. Protein intake is important for many aspects related to health, and current evidence suggests that some athletes require increased amounts of this nutrient. On the other hand, society's demand for more environmentally friendly products, focus on the search for alternative food sources more sustainable. This review aims to summarize the latest research on novel strategies and sources for greener and functional supplementation in sport nutrition. Alternative protein sources such as insects, plants or mycoproteins have proven to be an interesting substrate due to their high added value in terms of bioactivity and sustainability. Protein hydrolysis has proven to be a very useful technology to revalue by-products, such as collagen, by producing bioactive peptides beneficial on athletes performance and sport-related complications. In addition, it has been observed that certain amino acids from plant sources, as citrulline or theanine, can have an ergogenic effect for this target population. Finally, the future perspectives of protein supplementation in sports nutrition are discussed. In summary, protein supplementation in sports nutrition is a very promising field of research, whose future perspective lies with the search for alternatives with greater bioactive potential and more sustainable than conventional sources.

show abstract

“…However, few protein supplements made from chicken breast have been found. Furthermore, protein hydrolysates containing oligopeptides are absorbed better than intact proteins and AAs [19][20][21][22][23].…”

Section: Ffhdmentioning

confidence: 99%

Blood amino acid dynamics after ingestion of chicken-derived peptides in healthy subjects

Guo¹,

Yamamura²,

Sato³

2022

FFHD

View full text Add to dashboard Cite

Background: The rate of protein digestion and amino acid (AA) absorption determines the postprandial rise in circulating AA and modulates postprandial muscle protein synthesis (MPS) rates. Furthermore, it is necessary to consider the timing of protein ingestion, along with its quantity and quality, to regulate the blood AA concentration. Chicken breasts are a popular food among athletes as they are a good source of animal protein, containing sufficient essential amino acids (EAAs) and branched-chain amino acids (BCAAs). Low-molecular-weight chicken peptides (Cpep), a novel protein supplement, were isolated from chicken breasts. Blood AA dynamics, which have a significant influence on MPS rates, were observed and compared with commercially available whey- and soy-derived protein supplements.Objectives: We evaluated blood AA dynamics after Cpep intake compared with whey protein (WP), and soy protein (SP).Methods: Three groups of six healthy adult men volunteers (age 39 ± 10 years) ingested 0.3 g/kg (protein/body weight) of Cpep, WP, and SP. The concentrations of AA in the plasma were measured before and after the ingestion period and their kinetics were compared.Results: Cpep comprises free amino acids or peptides, and their average molecular weights are lower than those of WP and SP. The absorption dynamics of AA in the plasma were evaluated. After Cpep intake, EAA and BCAA concentrations peaked at 30 min and levels of EAA and BCAA were higher than those after WP and SP ingestion at 15 and 30 min, respectively. Conversely, the levels of total AA, EAA, and BCAA decreased 45 min after Cpep intake compared with WP and SP intakes. In contrast, WP and SP showed similar blood AA dynamics with a peak at 60 min.Conclusions: Cpep is absorbed significantly faster than WP and SP, making it a useful option for efficient protein intake to maintain and increase muscle mass.Keywords: chicken-derived peptides, blood amino acid dynamics, branched-chain amino acid, muscle protein synthesis

show abstract

The role of protein hydrolysates for exercise-induced skeletal muscle recovery and adaptation: a current perspective

Cited by 29 publications

References 175 publications

Hydrolyzed Collagen from Salmon Skin Increases the Migration and Filopodia Formation of Skin Keratinocytes by Activation of FAK/Src Pathway

Hydrolyzed Collagen from Salmon Skin Increases the Migration and Filopodia Formation of Skin Keratinocytes by Activation of FAK/Src Pathway

Protein and Sport: Alternative Sources and Strategies for Bioactive and Sustainable Sports Nutrition

Blood amino acid dynamics after ingestion of chicken-derived peptides in healthy subjects

Contact Info

Product

Resources

About