The role of reactive oxygen and antioxidant species in periodontal tissue destruction

Chapple, Iain L. C.; Matthews, J. B.

doi:10.1111/j.1600-0757.2006.00178.x

Cited by 745 publications

(864 citation statements)

References 445 publications

(629 reference statements)

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“…8-hydroxydeoxyguanosine (8-OHdG) is an oxidized nucleoside that is excreted in the bodily fluids with DNA repair. Several studies have demonstrated that 8-OHdG in bodily fluids can act as a biomarker of oxidative stress (Chiou et al 2003;Liu et al 2004;Wu et al 2004) and 8-OHdG is commonly used as a marker to evaluate oxidative DNA damage in disorders including chronic inflammatory diseases (Chapple and Matthews 2007).…”

Section: Introductionmentioning

confidence: 99%

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Çanakçı

Tatar

et al. 2009

Arch. Immunol. Ther. Exp.

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Introduction: Oxidative stress may contribute to the pathogenesis of periodontitis. However, the detailed molecular mechanism remains unclear. Both 8-hydroxydeoxyguanosine (8-OHdG) and mitochondrial DNA (mtDNA) deletion have been reported as early oxidative DNA damage markers. In this study, 8-OHdG levels in saliva and mtDNA deletions in gingival tissue of patients with chronic periodontitis (CP) were evaluated. Materials and Methods: Gingival tissue and whole saliva samples were collected from 32 patients with CP and 32 healthy control subjects. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level (CAL), and probing depth (PD) were measured. Using the ELISA and polymerase chain reaction methods, the salivary 8-OHdG levels and the 7.4-kbp and 5-kbp mtDNA deletions were examined. Results: The 5-kbp mtDNA deletion was detected in 20 of the 32 periodontitis patients (62.5%), but was not detected in the healthy controls. The mean value of 8-OHdG in the saliva of the periodontitis patients with deleted mtDNA was significantly higher than in the patients with non-deleted mtDNA (p<0.01). Also, significant correlation was found between the occurrence of the 5-kbp mtDNA deletion and salivary 8-OHdG levels (p<0.01). Similar correlations were detected between salivary 8-OHdG levels and age, PD, and CAL (p<0.01, p<0.05). Conclusion: Increased oxidative stress may lead to premature oxidative DNA damage in the gingival tissue of periodontitis patients and the salivary 8-OHdG level may signify premature oxidative mtDNA damage in diseased gingival tissue.

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Section: Introductionmentioning

confidence: 99%

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Çanakçı

Tatar

et al. 2009

Arch. Immunol. Ther. Exp.

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“…33,34 Nrf2 down-regulation in oPMNs of CP patients as cause for reduced PMN ability to neutralize ROS and withstand oxidative stress supports previous reports of increased oxidative damage in CP. 4,10 Interestingly, ingenuity pathway analysis predicted a more significant Nrf2 pathway down-regulation in blood PMNs of patients with CP compared with healthy controls. This seems to suggest that constitutive Nrf2 expression may be required for adequate PMN responses to subgingival biofilms, which is associated with periodontal health.…”

Section: Discussionmentioning

confidence: 99%

“…3 One mechanism suggested to contribute to periodontal tissue breakdown in CP is the increase in endogenousproduced oxidative stress observed in periodontitis lesions. 4,5 We have previously shown that CP patients refractory to conventional therapy present with more severe loss of tooth attachment when their oral polymorphonuclear neutrophils (oPMNs) produce high levels of ROS, suggesting increased oxidative stress in periodontal tissues through excess PMNderived ROS in these patients. 6 On the other hand, the protective role of antioxidants (AOs) in CP has been investigated by total antioxidant capacity measurements in whole saliva and gingival crevicular fluid (GCF) samples.…”

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confidence: 99%

Nuclear Factor Erythroid 2-Related Factor 2 Down-Regulation in Oral Neutrophils Is Associated with Periodontal Oxidative Damage and Severe Chronic Periodontitis

Sima

Aboodi

Lakschevitz

et al. 2016

The American Journal of Pathology

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The balance between reactive oxygen species and antioxidants plays an important role in periodontal health. We previously demonstrated that high reactive oxygen species production by oral polymorphonuclear neutrophils (oPMNs) in chronic periodontitis (CP) refractory to conventional therapy is associated with severe destruction of periodontium. Herein, we show that inhibition of antioxidant production through down-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway in oPMN, despite enhanced recruitment in the oral cavity, is associated with severe CP. Twenty-four genes in the Nrf2-mediated oxidative stress response pathway were down-regulated in PMNs of diseased patients. Downstream of Nrf2, levels of oPMN superoxide dismutase 1 and catalase were decreased in severe CP, despite increased recruitment. Nrf2 À/À mice had more severe loss of periodontium in response to periodontitis-inducing subgingival ligatures compared with wild-types. Levels of 8-hydroxydeoxyguanosine were increased in periodontal lesions of Nrf2 À/À mice, indicating high oxidative damage. We report, for the first time, Nrf2 pathway down-regulation in oPMNs of patients with severe CP. PMNs of CP patients may be primed for low antioxidant response in the context of high recruitment in the oral cavity, resulting in increased oxidative tissue damage. (Am J Pathol 2016 http://dx

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“…[1][2][3][4] Most recent data from the UK and Ireland suggest that the prevalence of chronic adult periodontitis remains high. The 1998 Adult Dental Health Survey in the UK found that 54% of dentate adults had some periodontal pocketing of 4 mm or more and 5% had deep pocketing of 6 mm or more, while 43% had some loss of attachment of 4 mm or more and 8% had loss of attachment of 6 mm or more.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3]7 It is well documented that many other chronic health conditions such as obesity, diabetes and cardiovascular disease can be prevented by a healthier diet. 8 There is also an increasing aware ness of the links between general health and oral health.…”

Section: Introductionmentioning

confidence: 99%

Attitudes and practices of dentists with respect to nutrition and periodontal health

Kelly

Moynihan

2008

Br Dent J

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The role of reactive oxygen and antioxidant species in periodontal tissue destruction

Abstract: Convention is to use • to signify an unpaired electron and ) or + to indicate the molecular charge, which may be +ve or )ve or indeed neutral (e.g. • OH).

Cited by 745 publications

References 445 publications

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Nuclear Factor Erythroid 2-Related Factor 2 Down-Regulation in Oral Neutrophils Is Associated with Periodontal Oxidative Damage and Severe Chronic Periodontitis

Attitudes and practices of dentists with respect to nutrition and periodontal health

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