The Role of Receptor Tyrosine Kinases in Primordial Germ Cell Migration

Silver-Morse, Louise; Li, Willis X.

doi:10.1007/978-1-61779-145-1_20

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…In a cross-sectional study of 5401 infertile men, subclinical hypothyroidism was significantly associated with an increased risk of an abnormal DNA fragmentation index (DFI) ( 35 ). The enzyme-linked receptor signaling pathway is related to cell reproduction, growth and differentiation processes ( 36 ). The enzyme-linked receptor is a transmembrane protein, and the intracellular domain usually exhibits some enzyme activity.…”

Section: Discussionmentioning

confidence: 99%

Novel underlying genetic markers for asthenozoospermia due to abnormal spermatogenesis and reproductive organ inflammation

Zhang,

Peng,

Wang

et al. 2024

Exp Ther Med

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Asthenozoospermia, a male fertility disorder, has a complex and multifactorial etiology. Moreover, the effectiveness of different treatments for asthenozoospermia remains uncertain. Hence, by using bioinformatics techniques, the present study aimed to determine the underlying genetic markers and pathogenetic mechanisms associated with asthenozoospermia due to abnormal spermatogenesis and inflammation of the reproductive tract. GSE160749 dataset was downloaded from the Gene Expression Omnibus database, and the data were filtered to obtain 1336 differentially expressed genes (DEGs) associated with asthenozoospermia. These DEGs were intersected with the epithelial mesenchymal transition datasets to yield 61 candidate DEGs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed, and the results revealed that these candidate DEGs were significantly enriched in the enzyme-linked receptor pathway and the thyroid hormone pathway. A protein-protein interaction network was constructed to identify the key genes of asthenozoospermia. A total of five key genes were identified, among which SOX9 was significantly upregulated, while HSPA4 , SMAD2 , HIF1A and GSK3B were significantly downregulated. These findings were validated by conducting reverse transcription-quantitative PCR for clinical semen samples. To determine the underlying molecular mechanisms, a regulatory network of transcription factors and miRNA-mRNA interactions was predicted. The expression levels of HSPA4 , SMAD2 and GSK3B were positively associated with several related etiological genes of asthenozoospermia. In total, five key genes were closely associated with the level and type of immune cells; higher levels of activated B cells and CD8 T cells were observed in asthenozoospermia. Thus, the findings of the present study may provide clues to determine the underlying novel diagnostic genetic markers and treatment strategies for asthenozoospermia.

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Section: Discussionmentioning

confidence: 99%

Novel underlying genetic markers for asthenozoospermia due to abnormal spermatogenesis and reproductive organ inflammation

Zhang,

Peng,

Wang

et al. 2024

Exp Ther Med

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“…Experiments using such approaches led to important conclusions regarding sex determination and intercellular communication in Drosophila. A recent publication [56] describes an updated protocol on the technique that was originally developed in the 1970s [57,58]. …”

Section: Genetic Methodsmentioning

confidence: 99%

Methods for studying oogenesis

Hudson

Cooley

2014

Methods

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Drosophila oogenesis is an excellent system for the study of developmental cell biology. Active areas of research include stem cell maintenance, gamete development, pattern formation, cytoskeletal regulation, intercellular communication, intercellular transport, cell polarity, cell migration, cell death, morphogenesis, cell cycle control, and many more. The large size and relatively simple organization of egg chambers make them ideally suited for microscopy of both living and fixed whole mount tissue. A wide range of tools is available for oogenesis research. Newly available shRNA transgenic lines provide an alternative to classic loss-of-function F2 screens and clonal screens. Gene expression can be specifically controlled in either germline or somatic cells using the Gal4/UAS system. Protein trap lines provide fluorescent tags of proteins expressed at endogenous levels for live imaging and screening backgrounds. This review provides information on many available reagents and key methods for research in oogenesis.

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