The Role of Scanning Electron Microscopy in the Direct Diagnosis of Onychomycosis

Yue, Xiumei; Wang, Aiping; Li, Qing

doi:10.1155/2018/1581495

Cited by 7 publications

(6 citation statements)

References 11 publications

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“…Роль гистологического исследования в диагностике микозов велика, так как наличие грибов, а также признаков их инвазии в ткани и кровеносные сосуды в гистологических препаратах является достоверным доказательством глубокой грибковой инфекции [25]. При подозрениях на наличие грибковой флоры и даже при клинической манифестации микозов микробиологические методы часто дают ложно-отрицательные результаты и не позволяют отдифференцировать контаминацию, колонизацию и инфекцию, а методы полимеразной цепной реакции (PCR) и иммуногистохимические реакции доступны не во всех лабораториях, поэтому сканирующая электронная микроскопия оценивается как важный метод диагностики [26], с помощью которого можно выявить грибковые поражения [27].…”

Section: клинические исследованияunclassified

Pathogenetic and clinical significance of fungal infection of the palmar aponeurosis in Dupuytren's contracture

Stupina,

Shchudlo,

Varsegova

et al. 2024

jour

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Introduction Among the generally accepted theories of the etiology and pathogenesis of palmar fascial fibromatosis, the role of infectious factors has not been considered; however, there are references to fungal skin lesions in patients with advanced contractures and several studies that identified fungal infection in surgical material from such patients.The purpose of the work was to assess the pathogenetic and clinical significance of fungal infection of the palmar fascia in Dupuytren's contracture.Materials and methods We studied 41 medical records of patients operated on for Dupuytren's contracture in stages II-IV. The surgical material was examined at the light-optical level (hematoxylin-eosin and methenamine-silver PASM stains) and with scanning electron microscopy.Results Fungal infection of the palmar aponeurosis was detected in 20 out of 41 patients; various types of tissue reaction to the introduction of fungi into the palmar aponeurosis and the blood vessels perforating it were found. Groups of patients without signs of fungal invasion (n = 21) and with signs of fungal infection of the palmar aponeurosis (n = 20) were comparable in clinical and demographic characteristics, but significantly differed in the rate of early relapses, 0 versus 25 % in the group with fungal infection (p = 0.02).Discussion The immunogenetic characteristics of patients with palmar fascial fibromatosis and characteristic skin lesions create general and local conditions for the introduction of fungal flora.Conclusion Histological detection of pseudohyphae of the genus Candida in the palmar aponeurosis and the lumens of blood vessels in patients with Dupuytren's contracture verifies invasive candidiasis; the relationship between fungal infection of the aponeurosis and an increased rate of early relapses of contracture has been statistically proven. To increase the duration of the relapse-free period and potentially the life expectancy of patients, consultations with infectious disease mycologists and correction of modifiable risk factors for candidiasis are necessary.

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Section: клинические исследованияunclassified

Pathogenetic and clinical significance of fungal infection of the palmar aponeurosis in Dupuytren's contracture

Stupina,

Shchudlo,

Varsegova

et al. 2024

jour

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“…Interaction between dermatophyte fungi was observed under the JSM-5310LV electron microscope (Jeol, Jepang). SEM was used to observe the visualization of ultrastructure and to take the image of dermatophyte [15,16].…”

Section: Ultrastructure Finding Of Dermatophytes Culturementioning

confidence: 99%

Mechanisms of Dermatophyte Invasion on New Zealand Rabbit Skin Models

et al. 2020

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Dermatophytosis is a dermatophyte fungi infection most commonly found in animals and humans. The first step of infection is started from the attachment of arthroconidium into stratum corneum. The attachment of fungi to the host cell is mediated by fungi adhesin and its interaction with the host receptor. The objective of this research was to develop the infection model of Microsporum gypseum and Microsporum canis by inoculating the macroconidia intradermally on the rabbit model. The macroconidia collection method from culture media was re-visualized as fungi ultrastructure using scanning electron microscopy (SEM). Skin lesion analysis was measured from clinical changes of the skin based on primary dermatophytosis signs such as circular alopecia with erythema and squamosal. Clinical confirmation test was done via skin sampling followed by histopathological examination using Methenamine Silver—Grocott’s (GMS) staining. As a result of this research, the in vivo infection model through direct infection of macroconidia applied intradermally was very effective in improving the direct infection to the invasion phase on the skin. This model confirmed the epidermal differentiation process and skin permeability showed primary lesion within 2 hours and aggravated up to 6 hours after inoculation. In conclusion, macroconidium is a potential source of infection to induce the dermatophytosis model and the severity of primary injection correlated with duration and the scale of clinical symptoms exhibited. This is a promising model for further research on the mechanism involved in dermatophyte infection.

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“…The SEM technique is non-invasive and provides accurate identification of changes that may occur in the surface morphology of biological samples [ 20 ], including protein dynamics [ 21 ], cell study [ 22 ], or organ fragments—small multicellular organisms on a micrometric (10−6 m) and nanometric (10−9 m) scale [ 23 ]. For example, SEM was used to evaluate the degree of nail damage in onychomycosis and also for preliminary identification of the involved pathogen, when the traditional mycological methods are negative [ 24 , 25 ]. X-ray diffraction was previously used to study the molecular structure of hard α-keratin from different sources, such as hair, wool, etc.…”

Section: Introductionmentioning

confidence: 99%

Raman Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy as Noninvasive Methods for Microstructural Alterations in Psoriatic Nails

Chiriac

Azoicăi

Coroabă³

et al. 2021

Molecules

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Psoriasis is a chronic inflammatory disease associated with immune system dysfunction that can affect nails, with a negative impact on patient life quality. Usually, nail psoriasis is associated with skin psoriasis and is therefore relatively simple to diagnose. However, up to 10% of nail psoriasis occurs isolated and may be difficult to diagnose by means of current methods (nail biopsy, dermoscopy, video dermoscopy, capillaroscopy, ultrasound of the nails, etc.). Since the nail is a complex biological tissue, mainly composes of hard α-keratins, the structural and morphological techniques can be used to analyze the human fingernails. The aim of this study was to corroborate the information obtained using Raman spectroscopy with those obtained by scanning electron microscopy (SEM) and X-ray diffractometry and to assess the potential of these techniques as non-invasive dermatologic diagnostic tools and an alternative to current methods.

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The Role of Scanning Electron Microscopy in the Direct Diagnosis of Onychomycosis

Cited by 7 publications

References 11 publications

Pathogenetic and clinical significance of fungal infection of the palmar aponeurosis in Dupuytren's contracture

Pathogenetic and clinical significance of fungal infection of the palmar aponeurosis in Dupuytren's contracture

Mechanisms of Dermatophyte Invasion on New Zealand Rabbit Skin Models

Raman Spectroscopy, X-ray Diffraction, and Scanning Electron Microscopy as Noninvasive Methods for Microstructural Alterations in Psoriatic Nails

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