The role of <scp>SDF</scp>‐1 and <scp>CXCR</scp>4 on odontoblastic differentiation in human dental pulp cells

Kim, D. S.; Kim, Y. S.; Bae, Won Kyung; Lee, H. J.; Chang, Seok Woo; Kim, W. S.; Kim, E. C.

doi:10.1111/iej.12182

Cited by 25 publications

(13 citation statements)

References 33 publications

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“…Chemotactic stimuli recruit various cell types to the injury site. The interaction between the chemokine SDF1 and its receptor CXCR4, expressed on the hDPSC surface, is a key factor in the recruitment of these cells to the damaged area [28,29]. The increase in CXCR4 expression on hDPSCs, found in the present study, may also be of interest.…”

Section: Discussionsupporting

confidence: 52%

Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering

et al. 2017

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Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim of obtaining a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal–vascular dental pulp fraction, using appropriate markers, and cultured. Growth curves, expression of bone-related markers, calcification and angiogenesis as well as an in vivo transplantation assay were performed. We found that hDPSCs proliferate, differentiate into osteoblasts and express high levels of angiogenic genes, such as vascular endothelial growth factor and platelet-derived growth factor A. Human DPSCs, after 40 days of culture, give rise to a 3D structure resembling a woven fibrous bone. These woven bone (WB) samples were analysed using classic histology and synchrotron-based, X-ray phase-contrast microtomography and holotomography. WB showed histological and attractive physical qualities of bone with few areas of mineralization and neovessels. Such WB, when transplanted into rats, was remodelled into vascularized bone tissue. Taken together, our data lead to the assumption that WB samples, fabricated by DPSCs, constitute a noteworthy tool and do not need the use of scaffolds, and therefore they are ready for customized regeneration.

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Section: Discussionsupporting

confidence: 52%

Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering

et al. 2017

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“…The present assays comprised a short-term 24h spheroid culture. It is known that longer culture periods of 7 days can affect the levels of SDF-1 during odontoblastic differentiation (Kim et al 2014). Indeed, longer culture conditions might lead to a differential response of SDF-1, in particular as hypoxia, DFO and L-MIM differentially modulate odontoblastic differentiation (Jiang et al 2014(Jiang et al , M€ uller et al 2015.…”

Section: Discussionmentioning

confidence: 99%

“…SDF‐1 is an important factor for cell migration and homing and is also involved in differentiation of odontoblasts (Kim et al . ). In addition to the pro‐angiogenic effects, pre‐conditioning supports cell homing and cell survival (Stubbs et al .…”

Section: Introductionmentioning

confidence: 97%

“…Furthermore, hypoxia pre-conditioning can modulate stromal cell-derived factor (SDF)-1 production in mesenchymal stem cells (Gong et al 2010). SDF-1 is an important factor for cell migration and homing and is also involved in differentiation of odontoblasts (Kim et al 2014). In addition to the pro-angiogenic effects, pre-conditioning supports cell homing and cell survival (Stubbs et al 2012, Najafi & Sharifi 2013, Liu et al 2014.…”

Section: Introductionmentioning

confidence: 99%

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Formation of spheroids by dental pulp cells in the presence of hypoxia and hypoxia mimetic agents

et al. 2017

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Aim To evaluate the impact of hypoxia and hypoxia mimetic agents (HMA) on the formation and activity of spheroids by dental pulp cells (DPC). Methodology DPC on agarose-coated plates were treated with hypoxia and the HMA dimethyloxallyl glycine (DMOG), desferrioxamine (DFO) and L-mimosine (L-MIM). Images of spheroids were taken directly after seeding and at 6 h and 24 h. Spheroid sizes were quantified by area measurement with ImageJ software. Viability was assessed with Live-Dead staining, MTT and resazurin-based toxicity assay. Production of VEGF, IL-8 and SDF-1 was evaluated using immunoassays. Data were analysed using KruskalWallis test and post hoc Mann-Whitney U-test. Results DPC formed spheroids in the presence of hypoxia, HMA and combined treatment with hypoxia and HMA. No pronounced difference in spheroid size was found in the groups treated with hypoxia, DMOG, DFO, L-MIM and the combination of hypoxia and the HMA relative to their normoxic controls (P > 0.05). Spheroids appeared vital in Live-Dead and MTT staining and the resazurin-based toxicity assay. Evaluation of protein production with immunoassays revealed significantly enhanced levels of VEGF and IL-8 (P < 0.05), but there was no significant effect on SDF-1 production (P > 0.05). Treatment with a combination of hypoxia and HMA did not further boost VEGF and IL-8 production (P > 0.05). Conclusions Pre-conditioning with hypoxia and HMA increased the pro-angiogenic capacity of spheroids whilst not interfering with their formation. Preclinical studies will reveal whether pre-conditioning of spheroids with hypoxia and HMA can effectively improve the efficiency of cell transplantation approaches for regenerative endodontics.

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“…Chemotactic stimuli recruit various cell types to the injury site. The interaction between the chemokine SDF1 and its receptor CXCR4 expressed on the hDPSCs' surface is a key factor in the recruitment of these cells to the damaged area (Jiang et al, 2008 ; Kim et al, 2013 ). The use of both sera induced an increase in CXCR4 expression on hDPSCs within 14 days of culture but this was more evident in cells expanded with NZ-FBS.…”

Section: Discussionmentioning

confidence: 99%

NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression

Spina¹,

Montella²,

Liccardo³

et al. 2016

Front. Physiol.

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Human dental pulp stem cells (hDPSCs), selected from the stromal-vascular fraction of dental pulp, are ecto-mesenchymal stem cells deriving from neural crests, successfully used in human bone tissue engineering. For their use in human therapy GMP procedures are required. For instance, the use of fetal bovine serum (FBS) is strongly discouraged in clinical practice due to its high risk of prions and other infections for human health. Alternatively, clinical grade sera have been suggested, including the New Zealand FBS (NZ-FBS). Therefore, the aim of this study was to evaluate the behavior of hDPSCs expanded in culture medium containing NZ-FBS. Since it was widely demonstrated hDPSCs display relevant capabilities to differentiate into osteogenic and angiogenic lineages, we performed a comparative study to assess if these features are also retained by cultivating the cells with a safer serum never tested on this cell line. hDPSCs were grown using NZ-FBS and conventional (C-FBS) for 7, 14, and 21 days, in both 2D and 3D cultures. Growth curves, expression of bone-related markers, calcification and angiogenesis were evaluated. NZ-FBS induced significant cell growth with respect to C-FBS and promoted an earlier increase expression of osteogenic markers, in particular of those involved in the formation of mineralized matrix (BSP and OPN) within 14 days. In addition, hDPSCs cultured in presence of NZ-FBS were found to produce higher mRNA levels of the angiogenic factors, such as VEGF and PDGFA. Taken together, our results highlight that hDPSCs proliferate, enhance their osteogenic commitment and increase angiogenic factors in NZ-FBS containing medium. These features have also been found when hDPSC were seeded on the clinical-grade collagen I scaffold (Bio-Gide®), leading to the conclusion that for human therapy some procedures and above all the use of GMP-approved materials have no negative impact.

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The role of SDF‐1 and CXCR4 on odontoblastic differentiation in human dental pulp cells

Abstract: Odontoblastic differentiation was stimulated by SDF-1 activation and repressed by SDF-1/CXCR4 inhibition. Thus, SDF-1/CXCR4 signalling may be a new therapeutic target and strategy to promote repair and regeneration in endodontics.

Cited by 25 publications

References 33 publications

Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering

Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering

Formation of spheroids by dental pulp cells in the presence of hypoxia and hypoxia mimetic agents

NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression

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