The role of serum levels of microRNA-21 and matrix metalloproteinase-9 in patients with acute coronary syndrome

Abstract

Acute coronary syndrome (ACS) is one of the leading causes of cardiovascular death. It seems that microRNA-21 and matrix metalloproteinase-9 implicated in the pathogenesis of cardiovascular diseases. The aim of this study was to investigate the role of circulating miR-21 and MMP-9 as biomarkers for ACS. Based on coronary angiography and electrocardiography results, 50 patients with ACS and 50 patients with stable coronary artery disease (stable CAD) were enrolled in this study. Samples were collected from patients and stored at -80 °C. Serum miR-21 gene expression was measured by quantitative real-time PCR method. Serum total MMP-9 was measured by enzyme-linked immunosorbent assay kit. Also, the activity of MMP-9 was measured by gelatin zymography. Patients with ACS had a significantly higher miR-21 level compared to the stable CAD ([Formula: see text] = 0.88 ± 0.06 and 0.31 ± 0.08 respectively, P < 0.001). At the same time, the serum levels and activity of MMP-9 were significantly higher in ACS patients compared to those with stable CAD (324.01 ± 17.57 and 204.6 ± 12.39 ng/mL, P < 0.001, and 2524.5 ± 131.3 and 1280.8 ± 19.6 units, P < 0.001, respectively). miR-21 expression levels were correlated positively with MMP-9, hs-CRP, and age and negatively with HDL-cholesterol (r = 0.33, P < 0.001, r = 0.22, P < 0.031, r = 0.26, P < 0.008, r = -0.32, P < 0.001, respectively). We concluded that increased serum expression of miR-21 and higher serum activity of MMP-9 may be useful indicators for ACS. However, we suggest further studies to be performed.

Anti-Angiogenic miR-222, miR-195, and miR-21a Plasma Levels in T1DM Are Improved by Metformin Therapy, Thus Elucidating Its Cardioprotective Effect: The MERIT Study

“…In a study of subclinical atherosclerosis, plasma miR-21a levels were positively correlated with systolic, diastolic blood pressure, CRP and cIMT, but negatively correlated with nitric oxide (NO) and eNOS [ 31 ]. Similarly, in acute coronary syndrome, miR-21 levels were increased and correlated with CRP, age and visfatin, but negatively correlated with HDL-cholesterol [ 32 ].…”

MicroRNA‐150 restores endothelial cell function and attenuates vascular remodeling by targeting PTX3 through the NF‐κB signaling pathway in mice with acute coronary syndrome

“…MicroRNAs (miRNAs) were considered as novel potential biomarkers in the detection of ACS (Ahlin et al, 2016). Recently, increasing evidence has been indicative of the diagnostic and prognostic value of miRNAs in ACS (Darabi et al, 2016;Wei et al, 2016).…”

Noncoding RNAs as Biomarkers for Acute Coronary Syndrome

“…Circulating miR-21 in patients with ACS was significantly higher than that in patients with stable coronary heart disease/non-ACS [15,39,40], and it was also found that miR-21 was associated with visfatin [39] and mmp-9 (mmp-9) [40], which may participate in the pathogenesis of ACS by jointly promoting inflammatory response [39]. When miR-21 was combined with miR-1 and miR-499, the diagnostic efficiency was significantly improved (AUC 0.94), which was superior to the hypersensitive troponin T (AUC 0.89) [15].…”