The role of signaling crosstalk of microglia in hippocampus on progression of ageing and Alzheimer's disease

Consistent specificity and efficiency of tamoxifen-mediated cre induction across ages

“…Age of Tam induction does not affect the trajectory of age-related changes in the brain: Once NuTRAP allele induction was determined to be equivalent in both ages of Tam induction and collection, we asked whether timing of Tam induction can affect biological endpoints of importance. We and others have identified significant transcriptional changes in hippocampal microglia with aging 40,48,49 . We compared gene expression data from the TRAP positive fractions of old mice following early Tam induction (n=7, 7 females) and old mice following late Tam induction (n=5, 5 females) to young mice following early Tam induction (n=5, 5 females) to analyze differential gene expression (One way ANOVA, Benjamini Hochberg Multiple Testing Correction q<0.1, Fold Change >|1.25|) with aging.…”

Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age

“…We and others have identified significant transcriptional changes in hippocampal microglia with aging. 40 , 46 , 47 We compared gene expression data from the TRAP positive fractions of old mice following early Tam induction (n = 7, 7 females) and old mice following late Tam induction (n = 5, 5 females) to young mice following early Tam induction (n = 5, 5 females) to analyze differential gene expression (One way ANOVA, Benjamini Hochberg Multiple Testing Correction q < 0.1, fold change >|1.25|) with aging. As visualized by hierarchical clustering, the translatomic differences with age of the two old groups are similar to each other ( Figure 4 A).…”