The Role of SIRT1 on Angiogenic and Odontogenic Potential in Human Dental Pulp Cells

Kim, Jong-Jin; Kim, Sun-Ju; Kim, Young-Suk; Kim, Sun‐Young; Park, Sang‐Hyuck; Kim, Eun-Cheol

doi:10.1016/j.joen.2012.04.006

Cited by 44 publications

(46 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, human dental pulp could be a desirable option as a cell source for potential therapeutic applications. Recently, we reported that heme oxygenase-1, lysyl oxidase, and SIRT1 are novel molecular targets of HDPC differentiation and may have clinical implications for regenerative endodontics [21,[22][23][24]. To the best of our knowledge, this is the first reported study examining the expression of PIN1 during odontogenic or adipogenic differentiation, the effects of activating PIN1 with Ad-PIN1, and inhibiting it using juglone on the differentiation of HDPCs.…”

Section: Discussionmentioning

confidence: 92%

The Role of PIN1 on Odontogenic and Adipogenic Differentiation in Human Dental Pulp Stem Cells

Lee

Shin²,

Jue³

et al. 2014

Stem Cells and Development

Self Cite

View full text Add to dashboard Cite

Recently, the involvement of PIN1, a peptidyl-prolyl cis/trans isomerase, has been reported in age-related bone homeostasis and adipogenesis. However, the role of PIN1 during odontogenic and adipogenic differentiation remains to be fully understood, particularly regarding human dental pulp stem cells (HDPSCs). Thus, in the present study, we have investigated the role of PIN1 in odontogenic and adipogenic differentiation of HDPSCs and signaling pathways possibly involved. PIN1 mRNA and protein level were upregulated in a time-dependent manner during adipogenic differentiation, increasing until 1 day of odontogenic induction and then steadily declined during odontogenic differentiation. Treatment of a known PIN1 inhibitor, juglone, significantly increased odontogenic differentiation as confirmed by alkaline phosphatase (ALP) activity, calcium deposition, and mRNAs induction of odontogenic markers [ALP, osteopontin (OPN), osteocalcin (OCN), dentin sialophosphoprotein (DSPP), and dentin matrix protein 1 (DMP-1)]. On the contrary, adipogenic differentiation was dramatically reduced upon juglone treatment, with concomitant downregulation of lipid droplet accumulation and adipogenic marker genes [peroxisome proliferation-activated receptor gamma (PPARγ), lipoprotein lipase (LPL), and adipocyte fatty acid-binding protein (AP2)]. In contrast to PIN1 inhibition, the overexpression of PIN1 via adenoviral infection (Ad-PIN1) in HDPSCs inhibited odontogenic differentiation but increased adipogenic differentiation, in which stem cell property markers such as stage-specific embryonic antigen-4 (SSEA-4) and STRO-1 were upregulated during odontogenic differentiation but downregulated in adiopogenic differentiation. Consistently, juglone-mediated inhibition of PIN1 augmented the osteogenic medium (OM)-induced activation of bone morphogenetic protein (BMP), Wnt/β-catenin, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and nuclear factor-kappa B (NF-κB) pathway, which response was reversed by Ad-PIN1. Moreover, juglone blocked the adipogenic induction medium-induced activation of PPARγ, C/EBPα, C/EBPβ, ERK, and NF-κB pathways, which was rescued by Ad-PIN1 infection. In summary, the present study shows for the first time that PIN1 acts as an important modulator of odontogenic and adipogenic differentiation of HDPSCs and may have clinical implications for regenerative dentistry.

show abstract

Section: Discussionmentioning

confidence: 92%

The Role of PIN1 on Odontogenic and Adipogenic Differentiation in Human Dental Pulp Stem Cells

Lee

Shin²,

Jue³

et al. 2014

Stem Cells and Development

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, the formation of mineral nodules and mRNA expression of DMP-1, DSPP, ON, OCN, and OPN have been used as markers of odontoblastic differentiation (21,22). HDPCs in teeth can differentiate into odontoblasts when cultured in an osteogenic medium, even in the absence of dexamethasone, which is known to induce differentiation (23,24).…”

Section: Discussionmentioning

confidence: 99%

“…This process is regulated by the interplay of angiogenic factors such as VEGF and fibroblast growth factor 2, which is essential for the odontoblast differentiation of immature pulp cells (20,22). Ang-1 is another family of growth factors that plays an important role in vascular development (26).…”

Section: Discussionmentioning

confidence: 99%

Odontoblastic Differentiation, Inflammatory Response, and Angiogenic Potential of 4 Calcium Silicate–based Cements: Micromega MTA, ProRoot MTA, RetroMTA, and Experimental Calcium Silicate Cement

Chang

Bae

et al. 2015

Journal of Endodontics

Self Cite

View full text Add to dashboard Cite

“…To overcome the disadvantage of primary cell cultures, we used immortalized cell line for HDPCs (23), which have kept cellular characteristics of their original primary HDPCs in the mineralization related gene expressions and high mineralization activity (14). Furthermore, these immortalized HDPCs can differentiate when cultured in an osteogenic/dentinogenic medium, even in the absence of dexamethasone, which is known to induce differentiation (29,30).…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Growth Hormone and Mineral Trioxide Aggregate on Growth, Differentiation, and Angiogenesis in Human Dental Pulp Cells

Yun

Chang

Park

et al. 2016

Journal of Endodontics

Self Cite

View full text Add to dashboard Cite

The Role of SIRT1 on Angiogenic and Odontogenic Potential in Human Dental Pulp Cells

Cited by 44 publications

References 33 publications

The Role of PIN1 on Odontogenic and Adipogenic Differentiation in Human Dental Pulp Stem Cells

The Role of PIN1 on Odontogenic and Adipogenic Differentiation in Human Dental Pulp Stem Cells

Odontoblastic Differentiation, Inflammatory Response, and Angiogenic Potential of 4 Calcium Silicate–based Cements: Micromega MTA, ProRoot MTA, RetroMTA, and Experimental Calcium Silicate Cement

Combined Effects of Growth Hormone and Mineral Trioxide Aggregate on Growth, Differentiation, and Angiogenesis in Human Dental Pulp Cells

Contact Info

Product

Resources

About