The role of stigma peroxidases in flowering plants: insights from further characterization of a stigma-specific peroxidase (SSP) from Senecio squalidus (Asteraceae)

McInnis, Stephanie; Emery, David C.; Porter, Robert P.; Desikan, Radhika; Hancock, John T.; Hiscock, Simon J.

doi:10.1093/jxb/erj182

Cited by 87 publications

(92 citation statements)

References 60 publications

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“…In a recent work, relatively large amounts of ROS, principally H 2 O 2 , have been detected in stigma papillae from several species across a range of different angiosperm groups. This effect has been related to possible functions such as pollen-pistil interaction, redox signaling, or resistance to pathogen (McInnis et al, 2006). The presence of PsTRXh1 in these tissues may be an additional indication of its involvement in ROS metabolism.…”

Section: Discussionmentioning

confidence: 99%

PsTRXh1 and PsTRXh2 Are Both Pea h-Type Thioredoxins with Antagonistic Behavior in Redox Imbalances

et al. 2006

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Section: Discussionmentioning

confidence: 99%

PsTRXh1 and PsTRXh2 Are Both Pea h-Type Thioredoxins with Antagonistic Behavior in Redox Imbalances

et al. 2006

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“…Moreover the expression level of CavPrx in styles pollinated with compatible pollen was 25% higher than in styles not pollinated or pollinated with incompatible pollen: this level of expression suggests a possible involvement in pollination events. One of the possible role of peroxidases in the pollen-stigma interaction could be the promotion of pollen tube penetration and growth within the stigma, by loosening stigma cell wall components [41]. Microarray analysis carried out by Tung et al [14] in stigmas and transmitting tracts of A. thaliana revealed the presence of a consistent group of genes predicted to encode proteins with N-terminal signal peptides, as found in CavPrx.…”

Section: Discussionmentioning

confidence: 97%

“…Their implication in wall loosening is also underlined by the link between stigma receptivity and presence of stigma surface peroxidases in several species [44]. It is well-known that the expression of peroxidases increases with flower development, reaching a peak in mature and receptive styles [41,43]. Moreover it is known that principal ROS (reactive oxygen species) in stigmas is H 2 O 2 [41] but its function is yet unknown.…”

Section: Discussionmentioning

confidence: 99%

“…It is well-known that the expression of peroxidases increases with flower development, reaching a peak in mature and receptive styles [41,43]. Moreover it is known that principal ROS (reactive oxygen species) in stigmas is H 2 O 2 [41] but its function is yet unknown. This contemporary presence of high level of H 2 O 2 and peroxidases in the mature styles suggests that they could interact, even if the course of action is still unknown [41].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover it is known that principal ROS (reactive oxygen species) in stigmas is H 2 O 2 [41] but its function is yet unknown. This contemporary presence of high level of H 2 O 2 and peroxidases in the mature styles suggests that they could interact, even if the course of action is still unknown [41]. Stigmatic peroxidases could be important for regulating levels of H 2 O 2 in stigmas, either by degradation or generation of H 2 O 2 [44].…”

Section: Discussionmentioning

confidence: 99%

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Isolation of a gene encoding for a class III peroxidase in female flower of Corylus avellana L.

et al. 2012

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Hazelnut is a monoecius species characterized by mid-winter blooming and sporophytic incompatibility. The molecular mechanisms at the basis of the female flower development and of the pollen-stigma interaction are little known, although pollination in this species is a critical factor to ensure good yield. Differential Display technique was used to study genes expressed during the female flower development, comparing styles before emergence from the bud and styles at full bloom. The full-length cDNA clone, designated CavPrx (Corylus avellana peroxidase) and isolated in mature styles, was characterized as a sequence encoding for a 330 amino acids protein, containing all the conserved features of class III peroxidases. CavPrx resulted expressed only in styles, with a peak in mature styles pollinated with compatible pollen. Class III peroxidases are expressed in several different plant tissue types and are involved in a broad spectrum of physiological processes. Until now, four peroxidases expressed in the stigma were identified in Arabidopsis thaliana and Senecio squalidus: they were assumed to be possibly involved in pollen-pistil interaction, pollen tube penetration/growth and/or in defence against pathogens. CavPrx is the first gene for a floral peroxidase isolated in hazelnut and its expression pattern suggests a possible role in the pollination process.

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Oxidative Stress and Redox Signalling in Plants

Hancock

2016

Encyclopedia of Life Sciences

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Cell signalling is a critically important aspect of biology, being the mechanism by which organisms, tissues and cells coordinate their activity and survive environmental change and stress. Numerous biomolecules are involved but small reactive compounds known as reactive oxygen species (ROS) and reactive nitrogen species (RNS) are key players in this intricate network of signalling. Such compounds are inherently toxic, with increased ROS leading to a condition known as oxidative stress. However, in plants, numerous physiological responses are modulated by the presence of ROS, such as hydrogen peroxide (H 2 O 2 ) and RNS, such as nitric oxide (NO), including root growth, stomatal closure and cell death. Proteins such as kinases, phosphatases and metabolic enzymes are able to be controlled by the presence of H 2 O 2 and NO. Often, thiol groups on proteins are the target for modulation, but the presence and interactions with other redox compounds such as glutathione also need to be considered. Key Concepts Stress responses in plants often lead to the accumulation of reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) and reactive nitrogen species (RNS) such as nitric oxide (NO). Cells maintain an internal environment which is very reducing, and if this is disrupted, for example by the addition of oxidising compounds, the result is known as oxidative stress. The presence of ROS is a main way to enhance oxidative stress conditions. ROS such as H 2 O 2 , as well as NO, are made in plants by dedicated enzymes, and there are mechanisms for the removal of these biomolecules too, such as through the action of antioxidants. Many physiological functions use ROS and RNS as part of their control mechanisms, including germination, root development, stomatal closure and programmed cell death. ROS and RNS are both known to modulate levels of gene expression, such that some genes are expressed to a higher extent, while expression for others is reduced. ROS and RNS can modulate the activity of proteins such as MAP kinases, phosphatases and metabolic enzymes. The covalent modification of thiol groups in proteins is a key way for ROS and RNS to partake in cell signalling, to alter protein function. Dedicated redox proteins such as thioredoxin are instrumental in the control of redox signalling in cells. Cell signalling involving ROS and RNS should not be considered in isolation, but the impact of other compounds such as glutathione and hydrogen sulfide also needs to be considered.

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The role of stigma peroxidases in flowering plants: insights from further characterization of a stigma-specific peroxidase (SSP) from Senecio squalidus (Asteraceae)

Cited by 87 publications

References 60 publications

PsTRXh1 and PsTRXh2 Are Both Pea h-Type Thioredoxins with Antagonistic Behavior in Redox Imbalances

PsTRXh1 and PsTRXh2 Are Both Pea h-Type Thioredoxins with Antagonistic Behavior in Redox Imbalances

Isolation of a gene encoding for a class III peroxidase in female flower of Corylus avellana L.

Oxidative Stress and Redox Signalling in Plants

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