The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots

Abstract

Abstract. Cultured shoots of Rosa 'Improved Blaze' were used to determine the effects of sucrose and inorganic nitrogen on adventitious root formation. Shoots grown in media containing high sucrose concentrations (146.07-262.93 mM) produced more and longer roots than those grown in media containing 0-87.64 mM sucrose. This response to sucrose was related to the metabolism of sucrose rather than its osmotic properties since the use of mannitol and 3-0-methyl-a-D-glucopyranoside as osmotic substitutes did not reproduce the effect on rooting. The number and length of roots increased when the shoots were grown in media with the nitrogen concentration of the MurashigeSkoog (MS) salt formulation reduced from 60 to 7.5 mM. Neither nitrate (NO-3) nor ammonium (NH~) alone at any of the concentrations tested had the effect on rooting that both had together in the ratio of the MS salt formulation. When the sucrose and nitrogen concentrations were both varied, the greatest rate of root initiation occurred on shoots grown in media with a high sucrose to nitrogen concentration ratio.

Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America

“…The differences were initially thought to be due to the higher sucrose concentration being used in MS medium than in B5, but this was not borne out by experimentation. Organogenesis in leafy spurge does not appear to be coupled closely to the nitrogen status of the culture: at least not to the extent reported for somatic embryogenesis in several other cell culture systems (Hyndman et al 1982, Kamada and Harada 1979, Masuda et al 1981, Thomas and Street 1972, Wetherell and Dougall 1976. Leafy spurge cells were capable of organogenesis in a wide range of reduced:oxidized nitrogen combinations, but the ratio used in MS medium (33:67) appears to be somewhat more favorable than others.…”

Role of Different Carbon Sources on in Vitro Micropropagation of Oxalis Corniculata (L.)

“…Glucose is then utilized first and followed by fructose. It was observed that more and larger roots with an increase in sucrose concentration from 7.64 to 262.93mM [14]. Sucrose was reported to act as morphogenetic trigger in the formation of axillary buds and branching of adventitious roots [15].…”

Evaluación de tres niveles de nitrógeno en medio cultivo, en las fases de enraizamiento in vitro y adaptación a sustrato de Rubus glaucus (BENTH)

“…B 4,88 mM (T3). Hyndman et al (1982) lograron obtener un mayor número y longitud de raíces in vitro, de R. hybrida cv. "Improved Blaze", al disminuir la concentración total de nitrógeno de las sales MS en el medio, así se logró la concentración de las otras sales constante.…”

“…Luna et al (2004), recomiendan el medio MS diluido 4 veces, para el enraizamiento in vitro de Ilex dumosa. Hyndman et al (1982) demostró que la disminución de la concentración de KNO 3 y NH 4 NO 3 fue un factor decisivo para el mejoramiento en el porcentaje de enraizamiento en rosa; lo que concuerda con Asadi et al (2009) …”