The Role of Synthetic Microbial Communities (SynCom) in Sustainable Agriculture

Shayanthan, Ambihai; Ordoñez, Patricia Ann C.; Oresnik, Ivan J.

doi:10.3389/fagro.2022.896307

Cited by 90 publications

(34 citation statements)

References 137 publications

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“…Nutrient competition might have been present between them, thus decreasing their total amount and ability to resist the infection of Rs. The genomic evolution and horizontal gene transfer may hinder the performance of the combination [48]. Besides, some microbes might change their microbial expression under different environmental conditions [48].…”

Section: Discussionmentioning

confidence: 99%

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Heritability of tomato rhizobacteria resistant to Ralstonia solanacearum

et al. 2022

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Background Ralstonia solanacearum (Rs) is a soilborne phytopathogen that causes bacterial wilt and substantial yield losses in many plants, such as tomatoes. A resistant tomato cultivar can recruit a beneficial microbiome from soil to resist Rs. However, whether this recruitment is inheritable from resistant parent to progeny has not been determined. Results In the present study, we investigated the rhizosphere microbiomes of tomatoes with clear kinship and different resistance against Rs. Resistant tomatoes grown with the additions of natural soil or its extract showed lower disease indexes than those grown in the sterile soil, demonstrating the importance of soil microbiome in resisting Rs. The results of 16S ribosomal RNA gene amplicon sequencing revealed that the resistant cultivars had more robust rhizosphere microbiomes than the susceptible ones. Besides, the resistant progeny HF12 resembled its resistant parent HG64 in the rhizosphere microbiome. The rhizosphere microbiome had functional consistency between HF12 and HG64 as revealed by metagenomics. Based on multi-omics analysis and experimental validation, two rhizobacteria (Sphingomonas sp. Cra20 and Pseudomonas putida KT2440) were enriched in HF12 and HG64 with the ability to offer susceptible tomatoes considerable protection against Rs. Multiple aspects were involved in the protection, including reducing the virulence-related genes of Rs and reshaping the transcriptomes of the susceptible tomatoes. Conclusions We found promising bacteria to suppress the tomato bacterial wilt in sustainable agriculture. And our research provides insights into the heritability of Rs-resistant tomato rhizobacteria, echoing the inheritance of tomato genetic material.

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Section: Discussionmentioning

confidence: 99%

“…The genomic evolution and horizontal gene transfer may hinder the performance of the combination [48]. Besides, some microbes might change their microbial expression under different environmental conditions [48]. Therefore, the functions of these microbes could be changed upon assembly, resulting in the loss of the biocontrol effect.…”

Section: Discussionmentioning

confidence: 99%

Heritability of tomato rhizobacteria resistant to Ralstonia solanacearum

et al. 2022

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“…Synthetic microbial communities (SynCom) has been reported as a highly reproducible tool for investigating the relationship between plants and bacteria ( Shayanthan et al, 2022 ). Follow-up studies will aim to isolate bacteria from infected lily grown under the continuous cropping system in order to detect specific strains and their pathogenic characteristics ( Yadav et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Rhizospheric microbial consortium of Lilium lancifolium Thunb. causes lily root rot under continuous cropping system

Dai

Singh²,

Gong³

et al. 2022

Front. Microbiol.

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Tiger lily (Lilium lancifolium Thunb.) is a cash crop with a long history of cultivation in China. Its roots have long been used as a valuable component of Chinese medicine. Continuous cropping, the conventional planting approach for tiger lily, often leads to severe root rot disease, but it is not yet clear how this planting method leads to root rot. In this study, we analyzed the rhizosphere microbiome and predicted microbial protein function in tiger lily planted with the continuous cropping method in three different geological types of soil. In order to explore the specific rhizosphere microbiota triggering root rot disease, tiger lily was compared to maize grown in a similar system, which showed no disease development. An analysis of the chemical elements in the soil revealed that the Pseudomonas and Streptomyces genera, with pathogenic functions, were dominant in the tiger lily rhizosphere. The lower soil pH of tiger lily compared to maize supports the accumulation of pathogenic bacteria in the tiger lily rhizosphere. Meanwhile, we discovered that bacteria of the Flavobacterium genus, with their predicted phosphate transport function, specifically accumulated in the maize rhizosphere. Our findings suggest that Pseudomonas and Streptomyces bacteria may result in continuous cropping–induced root rot disease in tiger lily and that Flavobacterium could serve to protect maize from pathogenic bacteria.

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“…Synergistic interactions in the plant microbiome are major contributors to plant resistance. The design and inoculation of synthetic microbial communities (SynComs) derived from native plant populations is a promising avenue for plant health promotion [ 178 , 186 , 189 , 190 , 191 ]. A noteworthy example of microbial synergism is displayed by the root endophyte Serendipita vermifera, which works with bacterial microbiota to confer protection against the soil-borne fungal pathogen, Bipolaris sorokiniana in Arabidopsis thaliana and barley; modulation of effector expression was observed for both the pathogenic and the endophytic fungus [ 192 ].…”

Section: Effectors and Plant Defensementioning

confidence: 99%

Microbial Effectors: Key Determinants in Plant Health and Disease

et al. 2022

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Effectors are small, secreted molecules that alter host cell structure and function, thereby facilitating infection or triggering a defense response. Effectoromics studies have focused on effectors in plant–pathogen interactions, where their contributions to virulence are determined in the plant host, i.e., whether the effector induces resistance or susceptibility to plant disease. Effector molecules from plant pathogenic microorganisms such as fungi, oomycetes and bacteria are major disease determinants. Interestingly, the effectors of non-pathogenic plant organisms such as endophytes display similar functions but have different outcomes for plant health. Endophyte effectors commonly aid in the establishment of mutualistic interactions with the plant and contribute to plant health through the induction of systemic resistance against pathogens, while pathogenic effectors mainly debilitate the plant’s immune response, resulting in the establishment of disease. Effectors of plant pathogens as well as plant endophytes are tools to be considered in effectoromics for the development of novel strategies for disease management. This review aims to present effectors in their roles as promotors of health or disease for the plant host.

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The Role of Synthetic Microbial Communities (SynCom) in Sustainable Agriculture

Cited by 90 publications

References 137 publications

Heritability of tomato rhizobacteria resistant to Ralstonia solanacearum

Heritability of tomato rhizobacteria resistant to Ralstonia solanacearum

Rhizospheric microbial consortium of Lilium lancifolium Thunb. causes lily root rot under continuous cropping system

Microbial Effectors: Key Determinants in Plant Health and Disease

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