The role of T-cells in neurobehavioural development: Insights from the immunodeficient nude mice

Bauer-Negrini, Guilherme; Deckmann, Iohanna; Schwingel, Gustavo Brum; Hirsch, Mauro Mozael; Fontes-Dutra, Mellanie; Carello‐Collar, Giovanna; Halliwell, Diane E.; Paraskevaidi, Maria; Morais, Camilo L. M.; Martin, Francis; Riesgo, Rudimar dos Santos; Gottfried, Carmem; Bambini-Júnior, Victorio

doi:10.1016/j.bbr.2021.113629

Cited by 2 publications

(2 citation statements)

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“…As we all know, the main difference between mice and nude mice is that nude mice have no thymus. The nude mice have been described as primary immunodeficiency mice without mature thymus-derived T cells ( 21 , 22 ). To demonstrate that the immediate ADRs might be mediated by thymus-derived T cells, we injected both BALB/c nude and BALB/c mice with SMI.…”

Section: Resultsmentioning

confidence: 99%

The immediate adverse drug reactions induced by ShenMai Injection are mediated by thymus-derived T cells and associated with RhoA/ROCK signaling pathway

et al. 2023

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IntroductionThe mechanism of the immediate adverse drug reactions (ADRs) induced by ShenMai injection (SMI) has not been completely elucidated. Within 30 minutes, the ears and lungs of mice injected with SMI for the first time showed edema and exudation reactions. These reactions were different from the IV hypersensitivity. The theory of pharmacological interaction with immune receptor (p-i) offered a new insight into the mechanisms of immediate ADRs induced by SMI.MethodsIn this study, we determined that the ADRs were mediated by thymus-derived T cells through the different reactions of BALB/c mice (thymus-derived T cell normal) and BALB/c nude mice (thymus-derived T cell deficient) after injecting SMI. The flow cytometric analysis, cytokine bead array (CBA) assay and untargeted metabolomics were used to explain the mechanisms of the immediate ADRs. Moreover, the activation of the RhoA/ROCK signaling pathway was detected by western blot analysis.ResultsIn BALB/c mice, the vascular leakage and histopathology results showed the occurrence of the immediate ADRs induced by SMI. The flow cytometric analysis revealed that CD4+ T cell subsets (Th1/Th2, Th17/Treg) were imbalanced. And the levels of cytokines such as IL-2, IL-4, IL12P70 and INF-γ increased significantly. However, in BALB/c nude mice, all the indicators mentioned above have not changed significantly. The metabolic profile of both BALB/c mice and BALB/c nude mice was significantly changed after injecting SMI, and the notable increase in lysolecithin level might have a greater association with the immediate ADRs induced by SMI. The Spearman correlation analysis revealed that LysoPC (18:3(6Z,9Z,12Z)/0:0) showed a significant positive correlation with cytokines. After injecting SMI, the levels of RhoA/ROCK signaling pathway-related protein increased significantly in BALB/c mice. Protein-protein interaction (PPI) showed that the increased lysolecithin levels might be related to the activation of the RhoA/ROCK signaling pathway.DiscussionTogether, the results of our study revealed that the immediate ADRs induced by SMI were mediated by thymus-derived T cells, and elucidated the mechanisms of such ADRs. This study provided new insights into the underlying mechanism of immediate ADRs induced by SMI.

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Section: Resultsmentioning

confidence: 99%

The immediate adverse drug reactions induced by ShenMai Injection are mediated by thymus-derived T cells and associated with RhoA/ROCK signaling pathway

et al. 2023

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“…Such a role has been confirmed by several studies. For example, Negrini et al reported that BALB/c nude mice show social disorders, accompanied by decreased expression of IFN-γ in the prefrontal cortex and the absence of T cells in the meninges [ 223 ]. The absence of a residential CD4 + CD69 + T lymphocyte subset in healthy murine brains resulted in suspended microglial activation, together with developmental defects of synapses and behavioral abnormalities [ 224 ].…”

Section: The Effects Of Neuroinflammation On Memory Impairment Via Th...mentioning

confidence: 99%

Neuroinflammation, memory, and depression: new approaches to hippocampal neurogenesis

Wu,

Zhang

2023

J Neuroinflammation

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As one of most common and severe mental disorders, major depressive disorder (MDD) significantly increases the risks of premature death and other medical conditions for patients. Neuroinflammation is the abnormal immune response in the brain, and its correlation with MDD is receiving increasing attention. Neuroinflammation has been reported to be involved in MDD through distinct neurobiological mechanisms, among which the dysregulation of neurogenesis in the dentate gyrus (DG) of the hippocampus (HPC) is receiving increasing attention. The DG of the hippocampus is one of two niches for neurogenesis in the adult mammalian brain, and neurotrophic factors are fundamental regulators of this neurogenesis process. The reported cell types involved in mediating neuroinflammation include microglia, astrocytes, oligodendrocytes, meningeal leukocytes, and peripheral immune cells which selectively penetrate the blood–brain barrier and infiltrate into inflammatory regions. This review summarizes the functions of the hippocampus affected by neuroinflammation during MDD progression and the corresponding influences on the memory of MDD patients and model animals.

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The role of T-cells in neurobehavioural development: Insights from the immunodeficient nude mice

Cited by 2 publications

References 49 publications

The immediate adverse drug reactions induced by ShenMai Injection are mediated by thymus-derived T cells and associated with RhoA/ROCK signaling pathway

The immediate adverse drug reactions induced by ShenMai Injection are mediated by thymus-derived T cells and associated with RhoA/ROCK signaling pathway

Neuroinflammation, memory, and depression: new approaches to hippocampal neurogenesis

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