The Role of TGFβ Signaling in Wound Epithelialization

Ramirez, Horacio; Patel, Shubham; Pastar, Irena

doi:10.1089/wound.2013.0466

Cited by 178 publications

(167 citation statements)

References 60 publications

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“…We found that miR-132 expression was significantly increased only by TGF-β1 and TGF-β2 ( Figure 2D), but not by the other factors (Supplemental Figure 2). It has been shown that TGF-β is upregulated in skin wounds and plays a crucial role in regulating keratinocyte migration and proliferation during reepithelialization (12). Here, we measured the expression of TGF-β in human wound biopsies and confirmed that both TGF-β1 ( Figure 2E) and TGF-β2 ( Figure 2F) were upregulated in skin wounds, although with different kinetics.…”

Section: Mir-132 Expression Is Induced By Tgf-β In Keratinocytessupporting

confidence: 67%

“…TGF-βs are pluripotent cytokines that maintain skin homeostasis, and both TGF-β1 and TGF-β2 are upregulated in wounds, where they promote keratinocyte migration during reepithelialization (reviewed in ref. 12). It has been shown that TGF-β inhibits epidermal keratinocyte proliferation (12), whereas here we found that miR-132, which is induced by TGF-β, actually increased keratinocyte proliferation.…”

Section: Discussioncontrasting

confidence: 47%

“…12). It has been shown that TGF-β inhibits epidermal keratinocyte proliferation (12), whereas here we found that miR-132, which is induced by TGF-β, actually increased keratinocyte proliferation. We hypothesize that induction of miR-132 expression may be a novel way for TGF-β to reduce its negative effect on keratinocyte growth during wound repair, and this will be interesting to test in a future study.…”

Section: Discussioncontrasting

confidence: 47%

“…Binding of TGF-β to its receptors TβR-II and TβR-I leads to the activation of downstream Smad proteins, which induce the expression of TGF-β target genes (reviewed in ref. 12). Recently, it was shown in pancreatic cancer cells that transcription factor Sp1 plays a major role in MIR132 gene expression (31).…”

Section: Discussionmentioning

confidence: 99%

“…Because of the antiproliferative and proinflammatory effects of HB-EGF, it may be crit- transition from the inflammatory to the proliferative phase. In chronic, nonhealing wounds, TGF-β signaling, which is an important inducer of miR-132 expression, is attenuated (12). This may result in the deregulation of miR-132 expression and in turn contribute to the characteristic phenotype of persistent inflammation and the failure to enter the normal proliferative phase (1,2).…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

MicroRNA-132 enhances transition from inflammation to proliferation during wound healing

Li¹,

Wang²,

Liu³

et al. 2015

J. Clin. Invest.

185

193

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Section: Mir-132 Expression Is Induced By Tgf-β In Keratinocytessupporting

confidence: 67%

Section: Discussioncontrasting

confidence: 47%

Section: Discussioncontrasting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

MicroRNA-132 enhances transition from inflammation to proliferation during wound healing

Li¹,

Wang²,

Liu³

et al. 2015

J. Clin. Invest.

185

193

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Self‐Assembly of Naturally Small Molecules into Supramolecular Fibrillar Networks for Wound Healing

Huang

Gong

Wang

et al. 2022

Adv Healthcare Materials

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Self-assemblies of bioactively natural compounds into supramolecular hydrogels without structural modifications are of interest to improve their sustained releases and bioavailabilities in vivo. However, it is still a formidable challenge to dig out such a naturally small molecule with a meticulous structure which can be self-assembled to form a hydrogel for biomedical applications. Here, a new hydrogel consisting only of gallic acid (GA) via 𝝅-𝝅 stacking and hydrogen bond interactions, whereas none of GA analogues can form the similar supramolecular hydrogels, is reported. This interesting phenomenon is intriguing to further investigate the potential applications of GA hydrogels in wound healing. Notably, this GA hydrogel has rod-like structures with lengths varying from 10 to 100 μm. The biocompatibility and antibacterial tests prove that this well-assembled GA hydrogel has no cytotoxicity and excellent antibacterial activities against Escherichia coli and Staphylococcus aureus. Moreover, the GA hydrogel can significantly accelerate the process of wound healing with or without bacterial infections by mediation of inflammation signaling pathways. It is believed that the current study may shed a new light on the design of a supramolecular hydrogel based on self-assemblies of naturally small molecules to improve their bioavailabilities and diversify their uses in biomedical applications.

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Development of a porcine model of skin and soft‐tissue infection caused by Staphylococcus aureus, including methicillin‐resistant strains suitable for testing topical antimicrobial agents

Raška,

Lipový,

Kobzová

et al. 2024

Anim Models and Exp Med

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BackgroundIn view of the ever‐increasing representation of Staphylococcus spp. strains resistant to various antibiotics, the development of in vivo models for evaluation of novel antimicrobials is of utmost importance.MethodsIn this article, we describe the development of a fully immunocompetent porcine model of extensive skin and soft tissue damage suitable for testing topical antimicrobial agents that matches the real clinical situation. The model was developed in three consecutive stages with protocols for each stage amended based on the results of the previous one.ResultsIn the final model, 10 excisions of the skin and underlying soft tissue were created in each pig under general anesthesia, with additional incisions to the fascia performed at the base of the defects and immediately inoculated with Staphylococcus aureus suspension. One pig was not inoculated and used as the negative control. Subsequently, the bandages were changed on Days 4, 8, 11, and 15. At these time points, a filter paper imprint technique (FPIT) was made from each wound for semi‐quantitative microbiological evaluation. Tissue samples from the base of the wound together with the adjacent intact tissue of three randomly selected defects of each pig were taken for microbiological, histopathological, and molecular‐biological examination. The infection with the inoculated S. aureus strains was sufficient during the whole experiment as confirmed by both FPIT and from tissue samples. The dynamics of the inflammatory markers and clinical signs of infection are also described.ConclusionsA successfully developed porcine model is suitable for in vivo testing of novel short‐acting topical antimicrobial agents.

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The Role of TGFβ Signaling in Wound Epithelialization

Cited by 178 publications

References 60 publications

MicroRNA-132 enhances transition from inflammation to proliferation during wound healing

MicroRNA-132 enhances transition from inflammation to proliferation during wound healing

Self‐Assembly of Naturally Small Molecules into Supramolecular Fibrillar Networks for Wound Healing

Development of a porcine model of skin and soft‐tissue infection caused by Staphylococcus aureus, including methicillin‐resistant strains suitable for testing topical antimicrobial agents

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