The role of the BAFF/APRIL system in the T cell-independent specific response to blood stage Plasmodium falciparum hemozoin

Dechkajorn, Wilanee; Benjathummarak, Surachet; Kumsiri, Ratchanok; Maneerat, Yaowapa

doi:10.1016/j.cyto.2018.05.037

Cited by 6 publications

(9 citation statements)

References 53 publications

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“…The reason for the observed trend of reduced APRIL mRNA levels is unclear. In our previous study 32 , we observed increased expression of APRIL, BAFF, BAFF-R, TACI, and BCMA in HZ-stimulated monocyte and B cell co-cultures. Therefore, our previous knowledge might contribute that APRIL is also play roles in falciparum malaria.…”

Section: Discussionmentioning

confidence: 60%

“…We previously demonstrated the TI production of HZ-specific IgG by human B cells co-cultured with monocytes, which was mediated through activation of the BAFF/APRIL system 32,33 . A number of previous studies have also reported activation of the BAFF/APRIL system by malaria infection; these include studies of patients with P. falciparum malaria [34][35][36][37] , patients with P. vivax malaria 36 mice with P. yoelii malaria 38,39 and in vitro studies of B cells stimulated by P. falciparum-derived antigens 33,40 .…”

Section: Discussionmentioning

confidence: 99%

“…Data were normalized to ACTB (β-actin) mRNA in the same sample. The results in the spleen of falciparum malaria patients are presented as the fold change in expression relative to the levels in the normal spleen samples using the 2 −(ΔΔCt) method 32 . The results for the lymph node tissues of the patients and tonsillitis were represented as ACTB mRNA ratio 29 .…”

Section: Tissue Preparation and Histopathological Examination Formalmentioning

confidence: 99%

“…To determine expression of mRNA encoding BAFF, APRIL, BAFF-R, TACI, BCMA, and β-actin, total RNA was extracted from FFPE tissues as described previously 51,52 . RT-qPCR were performed in duplicate 32,53 using a Luna Universal One-Step RT-qPCR kit (New England Biolabs, MA, USA) and a CFX96TM Real-Time PCR Detection System (Bio-Rad Laboratories, CA, USA). Data were normalized to ACTB (β-actin) mRNA in the same sample.…”

Section: Quantification Of B Cell Receptor Expressionmentioning

confidence: 99%

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The activation of BAFF/APRIL system in spleen and lymph nodes of Plasmodium falciparum infected patients

Dechkhajorn

Benjathummarak

Glaharn

et al. 2020

Sci Rep

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previous studies have reported activation of the B cell-activating factor (BAff)/a proliferation-inducing ligand (ApRiL) system in t independent immunity against malaria infection. Plasmodium falciparum (P. falciparum) infected animal model is not feasible. therefore, little is known about the occurrence of BAff/ApRiL system and changes in falciparum lymphoid tissues. this study aimed to investigate the expression of BAff/ApRiL system components in lymphoid tissues from P. falciparum infected patients. Spleen and lymph node samples from 14 patients were collected at autopsy. Normal spleens and bacterially infected tonsils served as controls. the protein and/or mRnA expression of BAff/ApRiL and their cognate receptors, BAff-R, tAci and BcMA, were determined by immunohistochemistry and Rt-qPCR, respectively. The spleens of the patients exhibited significantly higher BAFF-R protein expression than normal spleens. Although without appropriate control, BcMA protein was markedly observed only in the lymph nodes. BAFF and BCMA mRNA levels were also significantly elevated in the spleen tissues of the patients compared with normal spleens. the overall BAff-R protein levels in the lymphoid tissues of the patients correlated positively with parasitaemia. These findings are the first to confirm that BAFF/ ApRiL system activation in lymphoid tissues and is positively correlated with the parasitaemia levels in falciparum malaria. The B cell-activating factor (BAFF)/a proliferation-inducing ligand (APRIL) system consists of BAFF, APRIL, and three cognate receptors expressed on B cells: B cell maturation antigen (BCMA), transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) 1 , and BAFF receptor (BAFF-R) 2-4. The BAFF/ APRIL system plays multiple regulatory roles in the T cell independent (TI) immune response, including B cell activation, homeostasis, and survival 5. APRIL binds with high affinity to both BCMA and TACI, while BAFF has lower affinity for these receptors and binds mainly to BAFF-R (reviewed in Dillon et al. 6). Ligand binding triggers activation of diverse signalling pathways, including the mitogen-activated kinase and nuclear factor-κB pathways 7,8 to induced immunoglobulin heavy chain class-switch recombination (CSR), and IgG and IgA production by B cells 5. BAFF and APRIL are predominantly expressed by myeloid cells such as monocytes, macrophages, and dendritic cells 9 and can be induced or upregulated by cytokines such as interferon-α and-γ, transforming growth factor-β, interleukin-4 and −10 2,10,11. Spleen and lymph nodes play pivotal roles in the host response to malaria. For example, the spleen eliminates Plasmodium parasitised red blood cells (PRBCs), and immune cells in both organs produce effector cytokines and specific anti-malaria antibodies that promote clearance of infection 12,13. The BAFF/APRIL system plays well-characterised roles in numerous human disorders, including autoimmune diseases 14-19 , cancers 20 , and bacterial 21 , viral 22 , and fungal 23 infections. ...

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Tissue Preparation and Histopathological Examination Formalmentioning

confidence: 99%

Section: Quantification Of B Cell Receptor Expressionmentioning

confidence: 99%

See 2 more Smart Citations

The activation of BAFF/APRIL system in spleen and lymph nodes of Plasmodium falciparum infected patients

Dechkhajorn

Benjathummarak

Glaharn

et al. 2020

Sci Rep

Self Cite

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“…The packed blood cells were resuspended in D-PBS and used to isolate the peripheral mononuclear cells (PBMCs). These were separated by Isoprep gradient centrifugation (Robbins Scientific Corporation) as described previously [ 28 ]. Approximately 2 million PBMCs in TRIzol (Invitrogen™) were kept at –70°C for the qRT-PCR.…”

Section: Methodsmentioning

confidence: 99%

Interleukin-8 in Hyperlipidemia and Coronary Heart Disease in Thai Patients Taking Statin Cholesterol-Lowering Medication While Undergoing Coronary Artery Bypass Grafting Treatment

et al. 2020

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The role of interleukin-8 (IL-8), a pivotal chemokine in atherogenesis and coronary heart disease (CHD) development, is diverse and remains unclear. This cross-sectional study investigates the association of the IL-8 expression in hyperlipidemia (H) and CHD patients who have been treated with statin cholesterol-lowering drugs while undergoing coronary artery bypass grafting treatment. Fifty-five Thai volunteers including 13 normal (N), 24 H, and 18 CHD patients were enrolled for the investigation. All the CHD patients had been treated continuously with statin cholesterol-lowering medications since the disease was diagnosed and were undergoing coronary bypass grafting approximately one month later. Therefore, the CHD group was representative of a pathogenesis improvement in CHD. The IL8 mRNA expression was determined by real-time quantitative PCR in the peripheral blood mononuclear cells from heparinized blood. The plasma IL-8 levels were assessed by enzyme-linked immunosorbent assay. The result shows that the IL8 mRNA expression in the H group tended to increase; however, in the CHD group, there was a significant decrease (p=0.0111) compared to the N group. The IL8 mRNA expression and the plasma levels in the CHD group were significantly lower than those in the H group (p<0.05). A significant negative correlation between the IL8 mRNA (r = −0.499) or plasma IL-8 (r = −0.3875) expression and CHD progression was observed (p<0.05). In conclusion, the transcriptomic and the phenotypic IL-8 expression decreased significantly in the Thai CHD patients who had continuously received statin-group medications compared to the H and N group participants. Therefore, IL-8 should serve as a feasible marker and could be used to evaluate the therapeutic effects of statins and illustrate the pathology of CHD treatment.

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Hemozoin in Malarial Complications: More Questions Than Answers

Pham

Lamb

Deroost

et al. 2021

Trends in Parasitology

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The role of the BAFF/APRIL system in the T cell-independent specific response to blood stage Plasmodium falciparum hemozoin

Cited by 6 publications

References 53 publications

The activation of BAFF/APRIL system in spleen and lymph nodes of Plasmodium falciparum infected patients

The activation of BAFF/APRIL system in spleen and lymph nodes of Plasmodium falciparum infected patients

Interleukin-8 in Hyperlipidemia and Coronary Heart Disease in Thai Patients Taking Statin Cholesterol-Lowering Medication While Undergoing Coronary Artery Bypass Grafting Treatment

Hemozoin in Malarial Complications: More Questions Than Answers

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