The role of the CBL–CIPK calcium signalling network in regulating ion transport in response to abiotic stress

Thoday-Kennedy, Emily; Jacobs, Andrew K.; Roy, Stuart J.

doi:10.1007/s10725-015-0034-1

Cited by 66 publications

(44 citation statements)

References 122 publications

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“…Different CBLs interact specifically with different CIPKs, resulting in a vast array of potential CBL/CIPK module combinations and versatility of Ca 2+ responsiveness to this signalling system. Each potential CBL/ CIPK module generates specificity in the signalling pathway and functions for crosstalk or overlap in signalling pathways (Thoday-Kennedy et al, 2015). Most CBL-CIPK components have been thoroughly studied in the regulation of different abiotic stresstriggered signalling pathways (Cheong et al, 2003;Guo et al, 2002;Pandey et al, 2004Pandey et al, , 2005, but the function of the CBL-CIPK system in response to biotic stresses is still an under-studied area of research.…”

Section: Introductionmentioning

confidence: 99%

TaCIPK10 interacts with and phosphorylates TaNH2 to activate wheat defense responses to stripe rust

Liu

Guo

Zhang

et al. 2018

Plant Biotechnology Journal

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Summary Calcineurin B‐like interacting protein kinase (CIPKs) has been shown to be required for biotic stress tolerance of plants in plant‐pathogen interactions. However, the roles of CIPKs in immune signalling of cereal crops and an in‐depth knowledge of substrates of CIPKs in response to biotic stress are under debate. In this study, we identified and cloned a CIPK homologue gene TaCIPK10 from wheat. TaCIPK10 was rapidly induced by Puccinia striiformis f. sp . tritici ( Pst ) inoculation and salicylic acid (SA) treatment. In vitro phosphorylation assay demonstrated that the kinase activity of TaCIPK10 is regulated by Ca 2+ and TaCBL4. Knockdown TaCIPK10 significantly reduced wheat resistance to Pst , whereas TaCIPK10 overexpression resulted in enhanced wheat resistance to Pst by the induction of defense response in different aspects, including hypersensitive cell death, ROS accumulation and pathogenesis‐relative genes expression. Moreover, TaCIPK10 physically interacted with and phosphorylated TaNH2, which was homologous to AtNPR3/4. Silencing of TaNH2 in wheat resulted in enhanced susceptibility to the avirulent Pst race, CYR23, indicating its positive role in wheat resistance. Our results demonstrate that TaCIPK10 positively regulate wheat resistance to Pst as molecular links between of Ca 2+ and downstream components of defense response and TaCIPK10 interacts with and phosphorylates TaNH2 to regulate wheat resistance to Pst .

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Section: Introductionmentioning

confidence: 99%

TaCIPK10 interacts with and phosphorylates TaNH2 to activate wheat defense responses to stripe rust

Liu

Guo

Zhang

et al. 2018

Plant Biotechnology Journal

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“…The involvement of CBL-CIPK complexes as signalling components in salt stress has been well established (Batistič and Kudla 2009;Hashimoto et al 2012;Luan 2009;Mao et al 2016;Thoday-Kennedy et al 2015). Arabidopsis CIPKs found to be involved in salinity tolerance mechanisms of plants include CIPK1 (D'Angelo et al 2006), CIPK3 (Kim 2003), CIPK6 (Tripathi et al 2009), CIPK16 (Roy et al 2013) and CIPK24 (SOS2) (Liu et al 2000).…”

Section: Introductionmentioning

confidence: 99%

AtCIPK16 Mediates Salt Stress Through Phytohormones and Transcription Factors

Amarasinghe

Huang

Watson-Haigh

et al. 2020

Preprint

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Soil salinity causes large productivity losses for agriculture worldwide. "Next-generation crops" that can tolerate salt stress are required for the sustainability of global food production. Previous research in Arabidopsis thaliana aimed at uncovering novel factors underpinning improved plant salinity tolerance identified the protein kinase AtCIPK16. Overexpression of AtCIPK16 enhanced shoot Na + exclusion and increased biomass in both Arabidopsis and barley. Here, a comparative transcriptomic study on Arabidopsis lines expressing AtCIPK16 was conducted in the presence and absence of salt stress, using an RNA-Seq approach, complemented by AtCIPK16 interaction and localisation studies. We are now able to provide evidence for AtCIPK16 activity in the nucleus. Moreover, the results manifest the involvement of a transcription factor, AtTZF1, phytohormones and the ability to quickly reach homeostasis as components important for improving salinity tolerance in transgenics overexpressing AtCIPK16. Furthermore, we suggest the possibility of both biotic and abiotic tolerance through AtCIPK16, and propose a model for the salt tolerance pathway elicited through AtCIPK16. PrxR) (Baxter et al. 2014; Mittler et al. 2011); c) osmotic adjusters that can maintain low intracellular osmotic potential in plants under salt stress (for example proline, glycine betaine, free amino acids, sugars, polyamines and polyphenols) (Rosa et al. 2009); d) phytohormones that can facilitate a broad array of adaptive responses and long distance signalling (such as abscisic acid, indole acetic acid, cytokinins, gibberellic acid, salicylic acid, brassinosteroids, jasmonates, ethylene) (Fahad et al. 2015; Peleg and Blumwald 2011; Ryu and Cho 2015); and e) salt sensors including those that sense cytosolic Ca 2+ changes resulting from changes in the cytosol due to salinity and communicate the effects to downstream activating proteins (calcineurin-B-like proteins; CBLs and CBL-interacting protein kinases; CIPKs, calcium-dependent protein kinases; CDPKs, calmodulins; CaMs, calmodulin-like proteins; CAMLs, etc.) (Shabala et al. 2015).

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“…Among these proteins, CBLs, which are small Ca 2+ binding proteins, mainly interact with CBL-interacting protein kinase (CIPK) as an upstream factor for downstream signaling and in response to several abiotic factors, including salt, cold, drought, proton, reactive oxygen species (ROS), abscisic acid (ABA) and gibberellins (GA) (Thoday-Kennedy et al, 2015). CBLs are most similar to the regulatory B subunit of calcineurin (CNB), a protein phosphatase in animals (Liu and Zhu, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…The interaction between AtCBL10 and CIPK24 under salt stress also activates the Na + transporter to transfer Na + to the vacuole of shoots or leaves (Quan et al, 2007). AtCBL1, AtCBL5 , and AtCBL9 are also involved in the response signaling to salt, drought, osmotic and ROS stress (Thoday-Kennedy et al, 2015). AtCBL2 and AtCBL3 could regulate the activity of proton pump V-ATPase on the vacuole membrane to maintain the ion homeostasis in the cell.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Salinity Tolerance of Transgenic Rice Lines Harboring HsCBL8 of Wild Barley (Hordeum spontanum) Line from Qinghai-Tibet Plateau

et al. 2016

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Rice is more sensitive to salinity, particularly at its early vegetative and later productive stages. Wild plants growing in harsh environments such as wild barley from Qinghai-Tibet Plateau adapt to the adverse environment with allelic variations at the loci responsible for stressful environment, which could be used for rice genetic improvement. In this study, we overexpressed HsCBL8 encoding a calcium-sensor calcineurin B-like (CBL) protein in rice. The gene was isolated from XZ166, a wild-barley (Hordeum spontanum) line originated from Qinghai-Tibet Plateau. We found that XZ166 responded to high NaCl concentration (200 mM) with more HsCBL8 transcripts than CM72, a cultivated barley line known for salinity tolerance. XZ166 is significantly different from CM72 with nucleotide sequences at HsCBL8. The overexpression of HsCBL8 in rice resulted in significant improvement of water protection in vivo and plasma membrane, more proline accumulation, and a reduction of overall Na+ uptake but little change in K+ concentration in the plant tissues. Notably, HsCBL8 did not act on some genes downstream of the rice CBL family genes, suggesting an interesting interaction between HsCBL8 and unknown factors to be further investigated.

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The role of the CBL–CIPK calcium signalling network in regulating ion transport in response to abiotic stress

Cited by 66 publications

References 122 publications

TaCIPK10 interacts with and phosphorylates TaNH2 to activate wheat defense responses to stripe rust

TaCIPK10 interacts with and phosphorylates TaNH2 to activate wheat defense responses to stripe rust

AtCIPK16 Mediates Salt Stress Through Phytohormones and Transcription Factors

Characterization of Salinity Tolerance of Transgenic Rice Lines Harboring HsCBL8 of Wild Barley (Hordeum spontanum) Line from Qinghai-Tibet Plateau

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