The role of the mesonephros in the development of indifferent gonads and ovaries of the mouse

Upadhyay, Swati; Luciani, J. M.; Zamboni, Luciano

doi:10.1051/rnd:19790802

Cited by 72 publications

(43 citation statements)

References 18 publications

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“…According to some researchers (3,10), the mesonephros contributes cells to the ovarian stroma, and mesonephric tubules may give rise to the follicular cells. In the developing mouse ovary, the mesonephros consists of a connecting complex of tubules in extraovarian and intraovarian locations.…”

Section: Introductionmentioning

confidence: 99%

Apparent Mesonephric Duct (Rete Anlage) Origin for Cysts and Proliferative Epithelial Lesions in the Mouse Ovary

Long

2002

Toxicol Pathol

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Ovaries and adjacent parovarian (mesovarial) tissues of CD-1 mice of various ages were examined to characterize cystic and proliferative epithelial lesions of the ovary and parovarian tissues. Ovaries and adjacent tissues from 6 mice each at approximately 3 and 8 months of age were processed for light microscopy and step-sections (50-l intervals) of the entire tissue were examined. Tissues were collected from 40 mice each at 16 through 24 months of age and 3 step sections per mouse were examined. Mesonephri c duct remnants were found in all mice at 3 and 8 months. Ducts were usually in the mesovarial adipose tissue and near or within the ovarian hilus and were often loosely associated with smooth muscle of the mesovarial ligament. The epithelium of the ducts varied from low cuboidal to columnar, occasional individual cells were ciliated, and small papillary con gurations of epithelium were occasionally present. Ducts were dilated ( > 1 mm) more often in mice at 8 months compared to 3 months, and some were continuous with cystic spaces within the ovaries. As mice aged (16-24 months), dilation and cystic change in ducts and associated compression of ovarian tissue away from the ovarian hilus became common. The epithelium of dilated ducts was generally attened, but foci of cuboidal to columnar epithelium and/or occasional ciliated cells were present. Many ducts contained foci of hyperplastic and/or hypertrophic epithelium, and papillary projections of epithelium were occasionally found. Some of the latter lesions were consistent with a diagnosis of papillary cystadenoma. Hyperplasia of associated bromuscular stroma was limited to a few apparently extraovarian ducts. The results of this study indicate that remnant mesonephric ductular structures are common in and adjacent to the ovaries of CD-1 mice. As mice age, these ducts become dilated and, in some, the epithelium becomes hyperplastic and /or hyperptrophic . These mesonephri c duct remnants appear to be a common source of ovarian and parovarian cyts cysts and epithelial neoplasms of the ovary in mice.

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Section: Introductionmentioning

confidence: 99%

Apparent Mesonephric Duct (Rete Anlage) Origin for Cysts and Proliferative Epithelial Lesions in the Mouse Ovary

Long

2002

Toxicol Pathol

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“…At puberty, preventer activity in the testis would decrease, thus releasing the germinal cells to the meiotic process (3,5). We recently found, however, that the somatic cells ofthe seminiferous cords ofthe testis are mesonephric in origin (9)(10)(11) and, in fact, derive from the same cellular elements that in the female differentiate into the precursors of the granulosa cells (12,13). The demonstration that the somatic cells of the male and female sex cords have the same mesonephric origin and identical differentiation patterns makes it much less easy to accept the hypothesis that they may be the source of substances having antagonistic influences on the germinal cells insofar as meiotic maturation is concerned.…”

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confidence: 99%

Ectopic germ cells: natural model for the study of germ cell sexual differentiation.

Upadhyay¹,

Zamboni²

1982

Proc. Natl. Acad. Sci. U.S.A.

132

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In the course of a study on the morphogenesis of the adrenal gland in random-bred Swiss albino mice, we noted the presence of ectopic germ cells in the adrenal cortexes and medullas in animals of both sexes, from day 12 1/2 of fetal development to postnatal day 12. Up to day 15 of fetal development, the cells exhibited characteristics of primordial germ cells. At day 17, and irrespective of the sex of the fetus, they all entered meiosis in synchrony with those in the ovary. Postnatally, in females as well as males, all ectopic germ cells displayed morphologic characteristics identical to those of young oocytes in unilaminar ovarian follicles. No germinal elements were seen in the adrenal glands past day 12 of life. Our study shows that mammalian germ cells are capable of undergoing sustained differentiation outside the gonads and that, in ectopic sites, they all differentiate into oocytes as they normally would in the ovary, even in males.

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“…Descriptive morphological studies have, furthermore, indicated that mesonephric cell contribution to the developing gonad is independent of the sex. In developing gonads of numerous mammalian species, the presence of 'cell streams' connecting the mesonephros tubuli and the developing gonad is suggested to indicate mesonephric cell contribution to both the ovarian and the testicular somatic cell population [mouse: Byskov et al, 1977;Byskov, 1978a;Upadhyay et al, 1979Upadhyay et al, , 1981Kanai et al, 1989;Karl and Capel, 1995;rat: Stein and Anderson, 1979;pig: Byskov et al, 1986;sheep: Zamboni et al, 1979sheep: Zamboni et al, , 1981Tisdall et al, 1999;human: Wartenberg, 1982;Wartenberg et al, 1991]. The mesonephric part of such 'cell streams' has been proposed to be the predominant cell contributor due to a comparable high mitotic activity [Stein and Anderson, 1979].…”

Section: Discussionmentioning

confidence: 99%

“…Studies of several species have suggested mesenchymal tissue, coelomic epithelium and/or the mesonephros as being the only or participating source of the gonadal somatic cells. These studies are based on experimental evidence [Byskov, 1974;Byskov et al, 1977] or morphological indications [Gondos, 1969;Merchant, 1975;Pelliniemi, 1976;Byskov, 1978a;Upadhyay et al, 1979Upadhyay et al, , 1981Zamboni et al, 1979;Wartenberg, 1982;Yoshinaga et al, 1988;Kanai et al, 1989;Francavilla et al, 1990;Smith and Mackay, 1991;Wartenberg et al, 1991;Karl and Capel, 1995;Magras et al, 1997;Tisdall et al, 1999]. A more direct way of examining somatic cell contribution from either the coelomic epithelium or the mesonephros has been attempted by tracing the migration of cells carrying a marker MerchantLarios et al, 1993;Martineau et al, 1997;Brennan et al, 1998;Karl and Capel, 1998;Merchant-Larios and Moreno-Mendoza, 1998;Tilmann and Capel, 1999;Albrecht et al, 2000].…”

Section: Introductionmentioning

confidence: 99%

Somatic Cell Exchange Occurs between Mouse Fetal Gonads and Mesonephroi during in vitro Culture

Nielsen

Byskov²

2001

Cells Tissues Organs

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Mesonephric cell contribution to the developing mouse gonad has previously been shown to be male-specific in in vitro culture models. This study shows that somatic cell exchange occurs between fetal mouse mesonephroi and gonads during in vitro culture of assembled tissues. Assembling gonads with cell marker-carrying mesonephroi and vice versa identified cell exchange. Two different cell markers were used. One of the markers was the thymidine analogue bromodeoxyuridine (BrdU), which was added to the culture medium during an initial culture period before in vitro culture of assembled tissues in medium without BrdU. The other was bacterial β-galactosidase, which is expressed ubiquitously in tissues from the transgenic mouse strain ROSA26. The tissues were taken from either 10.5- or 11.5-day-old mouse fetuses, and the assembled tissues were cultured for 36–60 h. Both cell markers demonstrated the occurrence of a cell exchange in both directions between the mesonephros and the gonad. The cell contribution was found to be minor and independent of the initial age of the tissue and hours in culture. Further, mesonephric cell contribution was not male-specific. Cell exchange between the two tissues may be due to growth between the assembled tissues rather than active signal-regulated migration. It is suggested that in vitro culture models may not be applicable for investigating mesonephric cell contribution to the developing mouse gonad.

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The role of the mesonephros in the development of indifferent gonads and ovaries of the mouse

Cited by 72 publications

References 18 publications

Apparent Mesonephric Duct (Rete Anlage) Origin for Cysts and Proliferative Epithelial Lesions in the Mouse Ovary

Apparent Mesonephric Duct (Rete Anlage) Origin for Cysts and Proliferative Epithelial Lesions in the Mouse Ovary

Ectopic germ cells: natural model for the study of germ cell sexual differentiation.

Somatic Cell Exchange Occurs between Mouse Fetal Gonads and Mesonephroi during in vitro Culture

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