The role of the seed tuber in determining partial resistance to potato blackleg caused byErwinia spp.

Allefs, Sjefke; Dooijeweert, W. van; Jong, E.R. de; Prummel, W.; Hoogendoorn, Coosje

doi:10.1007/bf01874765

Cited by 7 publications

(11 citation statements)

References 21 publications

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“…In this study, contamination of progeny tubers with Eca may have occurred late in the growing season due to a slow built up of Eca populations in the mother tubers to levels high enough for invading stolons and, consequently, infection of progeny tubers. This is in agreement with the absence of Eca in progeny tubers of early potato cultivars, which show an rapid average mother tuber decay in the field, such as Alcmaria, Arinda and Venouska (Allefs et al, 1995). The contrary was found for the late potato cultivars Hertha, Karnico 6,69 + ----+-= positive reaclion, -~ negative reaction, '' The detection thresholds of dilution plating were > ID" and > 10^ cells/mi before and after enrichment, respectively.…”

Section: Relation Between Blaekleg Incidence and Transmission Of Ecasupporting

confidence: 88%

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Comparison of Methods for Detection of Erwinia carotovora ssp. atroseptica in Progeny Tubers Derived from Inoculated Tubers of Solanum tuberosum L.

Fraaije

Birnbaum

Bulk

1996

Journal of Phytopathology

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Several techniques for detection of Erwinia carotovora ssp. atroseptica (Eca) on potato tubers were used to study the transmission of Eca from inoculated mother tubers into progeny tubers for 12 different potato cultivars. The sensitivity of the polymerase chain reaction (PCR) for a direct detection of Eca in crude peel extracts was only 107‐108 cells/ml, due to inhibition of PCR amplification by potato tuber‐derived compounds. With dilution plating and ELLSA, 105 and 107 cells/ml were detected, respectively, Immunofluorescence cell staining (IF), with a detection threshold of 104‐105 cells/ml, was shown to be most sensitive for direct detection, and transmission of Eca was found for six potato cultivars, whereas with the other techniques tested no transmission was found. After 48 h enrichment of peel extract in polypectate medium at 20 C, IF and PCR detected an initial inoculum concentration of 10 Eca cells/ml, whereas 102, 104 and 104 cells/ml were detected in dilution plating, ELISA and conductimetry, respectively. With dilution plating, PCR and IF, performed after enrichment, transmission of Eca was found for, respectively, four, six and seven out of 12 potato cultivars tested. No transmission was found using ELISA and conductimetry, due to their lower sensitivity, Erwinia carotovora ssp, carotovora (Ecc) was only detected in the progeny tubers of control plants. No relation was found between the transmission of Eca to progeny tubers and blackleg incidence in the field.

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Section: Relation Between Blaekleg Incidence and Transmission Of Ecasupporting

confidence: 88%

“…The results of blackleg development in the field of the cultivars tested were similar to those found by Allefs et al (1995). Cultivars Desiree, Arinda, Karnico and Producent were the most resistant, i.e.…”

Section: Relation Between Blaekleg Incidence and Transmission Of Ecasupporting

confidence: 82%

Comparison of Methods for Detection of Erwinia carotovora ssp. atroseptica in Progeny Tubers Derived from Inoculated Tubers of Solanum tuberosum L.

Fraaije

Birnbaum

Bulk

1996

Journal of Phytopathology

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“…Several methods have been described for the screening of tuber tissue resistance (see Allefs et al, 1995a) or stem tissue resistance (Hidalgo & Echandi, 1982;Hossain & Logan, 1983;Wastie, 1984;Lapwood & Read, 1986). In a previous study (Allefs et al, 1995b), it was shown that tuber tissue resistance of stored tubers of a set of 12 cultivars was not very well correlated with resistance to blackleg. Resistance of mother tuber tissue as expressed during development and growth of plants in the field, however, seemed to be of importance.…”

Section: Introductionmentioning

confidence: 99%

“…Here we present further evidence for the existence of a mechanism of resistance which is located in the stem base. Furthermore, we screened the same set of 12 cultivars for above-ground stem tissue resistance to Eca and Ech and studied the role of stem base resistance, stem tissue resistance, tuber tissue resistance (Allefs et al, 1995a, in press) and mother tuber decay in the field (Allefs et al, 1995b) as components in determining partial resistance to blackleg in the field. The consequences of the results for resistance breeding are discussed.…”

Section: Introductionmentioning

confidence: 99%

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

et al. 1996

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The various phases of the infection process in potato blackleg, caused by pectolytic Erwinia spp., were interpreted as components of partial resistance and studied in detail. Mother tubers of glasshouse-grown plants of two potato cultivars were inoculated with antibiotic resistant marker strains of Erwinia carotovora subsp. atroseptica (Eca) or E. chrysanthemi (Ech). Stem tissue samples of these plants were collected 3, 7, 14 and 21 days after inoculation and subsequently analysed for the presence of the bacteria. As soon as 3 days after inoculation, Erwinia bacteria were detected in the stem tissue of the plants. However, blackleg developed only when the rotting mother tuber tissue reached the sites where the stems were attached to the mother tuber. An inoculation method was developed for determining cultivar differences in the incidence and rate at which rot in tuber cylinders proceeds to sprouts on top of such cylinders. A set of 12 cultivars was screened for this putative component of resistance which was called 'stem base resistance'. Significant differences for stem base resistance were found among the cultivars. These differences were clearer when inoculation was carried out with Ech in contrast to Eca. Glasshouse-grown plants of the same set of cultivars were also screened for resistance of the above ground part of the stem. Cultivars differed significantly for this so-called 'stem tissue resistance', but results of some cultivars were not consistent among years of testing. Multiple regression analysis revealed that stem base resistance, stem tissue resistance, tuber tissue resistance and resistance of mother tubers in the field account for 63% (Eca) and 75% (Ech) of the variance found after screening for resistance to blackleg in the field. It was concluded that if results of this study are extrapolated to any set of potato clones, selection for resistance in breeding programmes under laboratory or glasshouse conditions, would be most efficient when directed to clonal differences for stem base resistance.

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“…This observation can be related to the differences in resistance expression of the cultivar depending on the organ tested. Several authors noted that one of the difficulties in screening cultivars for resistance to pectinolytic Erwinia was related to the lack of correlation between stem and tuber resistance (Allefs et al, 1995), and to the different performance of inoculation methods, leading to different rankings of potato cultivars (Bain & Pérombelon, 1988). The difficulty of detecting the bacterium on diseased organs (stolons and tubers) by PCR could be explained by inappropriate storage of the samples.…”

Section: Discussionmentioning

confidence: 99%

Internal colonization pathways of potato plants by Erwinia carotovora ssp. atroseptica

Hélias¹,

Andrivon²,

Jouan³

2000

Plant Pathology

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Transmission of pectinolytic Erwinia species from infected mother tubers to daughter tubers has been studied mainly through detection tests, carried out at harvest, on limited samples of tubers produced by plants grown from artificially inoculated mother tubers. However, detection has not been performed on samples collected at different stages of crop development, in order to follow the contamination progress in different organs through the plants to the progeny tubers. In this study the bacterial contamination of progeny tubers was investigated by detecting Erwinia carotovora ssp. atroseptica in different symptomless plant organs (stolons, stems, progeny tubers) and in the parts with or without symptoms of diseased stems, collected at various stages of crop development. Infection levels in below‐ and above‐ground organs of plants of two cultivars differing in their resistance to Erwinia, infected by either vacuum infiltration or sand wounding, were monitored throughout the growing season and at harvest using DAS‐ELISA and PCR. Detection tests showed that healthy organs from symptomless plants were less frequently contaminated than symptomless organs from diseased plants, and that stolons were precociously and more frequently contaminated than stems and daughter tubers, irrespective of the health of the plant. Stem infections were shown to progress latently in the stem, bacteria usually being recovered 10–15 cm past visible lesions. In many cases, typical aerial stem‐rot symptoms could be related to this upward movement of bacteria from the infected mother tuber. Daughter tubers without symptoms were shown to be frequently contaminated, usually at heel ends, suggesting internal contamination from mother tuber to progeny.

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The role of the seed tuber in determining partial resistance to potato blackleg caused byErwinia spp.

Cited by 7 publications

References 21 publications

Comparison of Methods for Detection of Erwinia carotovora ssp. atroseptica in Progeny Tubers Derived from Inoculated Tubers of Solanum tuberosum L.

Comparison of Methods for Detection of Erwinia carotovora ssp. atroseptica in Progeny Tubers Derived from Inoculated Tubers of Solanum tuberosum L.

Components of partial resistance to potato blackleg caused by pectolytic Erwinia carotovora subsp. atroseptica and E. chrysanthemi

Internal colonization pathways of potato plants by Erwinia carotovora ssp. atroseptica

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