The Role of the Urothelium and ATP in Mediating Detrusor Smooth Muscle Contractility

Santoso, Aneira Gracia Hidayat; Sonarno, Ika Ariyani Bte; Arsad, Noor Aishah Bte; Liang, Willmann

doi:10.1016/j.urology.2010.06.040

Cited by 16 publications

(14 citation statements)

References 23 publications

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“…Rat detrusor stripped of urothelium exhibits increased contractile responses to carbachol compared with strips with intact urothelium. The addition of ATP to the tissue bath resulted in reduced contractile responses, similar to strips retaining urothelium [41], which was in agreement with previous studies suggesting a urothelially mediated purinergic inhibitory effect on detrusor activity. Conversely, Munoz et al [23] demonstrated diminished tension responses to field stimulation, carbachol, and the ATP analogue α,β-methylATP in the absence of urothelium compared with intact rat bladder strips.…”

Section: Purinergic Signaling Purinoceptors and The Bladdersupporting

confidence: 91%

Purinoceptors and Bladder Dysfunction

Smith

2011

Curr Bladder Dysfunct Rep

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Purinergic signaling is increasingly appreciated as an important contributor to the normal bladder functions of urine storage and voiding. The translation of disease and injury to abnormal sensory and motor function, resulting in lower urinary tract symptoms, is in part mediated by the interaction of adenosine 5′-triphosphate and related purines with specific purinergic receptors (purinoceptors). Identification and clarification of purinoceptor-based processes are important research goals aimed at novel therapeutic targets for functional disorders inadequately served by standard therapies. This review presents an update on recent developments in the understanding of the purinoceptors and bladder dysfunction.

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Section: Purinergic Signaling Purinoceptors and The Bladdersupporting

confidence: 91%

Purinoceptors and Bladder Dysfunction

Smith

2011

Curr Bladder Dysfunct Rep

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“…Electrophysiological studies of suburothelial cells isolated from the human bladder showed that they were electrically active, responsive to ATP and perhaps coupled to neighbouring cells via gap junctions [642]. The urothelium and ATP suppressed carbachol-induced contractions of rat detrusor smooth muscle to a similar extent and this was interpreted to suggest an inhibitory role for ATP released from urothelium to modulate detrusor smooth muscle activity [597].…”

Section: Ectoenzymatic Breakdown Of Atpmentioning

confidence: 97%

Purinergic signalling in the urinary tract in health and disease

Burnstock

2013

Purinergic Signalling

142

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Purinergic signalling is involved in a number of physiological and pathophysiological activities in the lower urinary tract. In the bladder of laboratory animals there is parasympathetic excitatory cotransmission with the purinergic and cholinergic components being approximately equal, acting via P2X1 and muscarinic receptors, respectively. Purinergic mechanosensory transduction occurs where ATP, released from urothelial cells during distension of bladder and ureter, acts on P2X3 and P2X2/3 receptors on suburothelial sensory nerves to initiate the voiding reflex, via low threshold fibres, and nociception, via high threshold fibres. In human bladder t h e p u r i n e rg i c c o m p o n e n t o f p a r a s y m p a t h e t i c cotransmission is less than 3 %, but in pathological conditions, such as interstitial cystitis, obstructed and neuropathic bladder, the purinergic component is increased to 40 %. Other pathological conditions of the bladder have been shown to involve purinoceptor-mediated activities, including multiple sclerosis, ischaemia, diabetes, cancer and bacterial infections. In the ureter, P2X7 receptors have been implicated in inflammation and fibrosis. Purinergic therapeutic strategies are being explored that hopefully will be developed and bring benefit and relief to many patients with urinary tract disorders.

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“…Both urothelium‐intact (+UE) and urothelium‐denuded (−UE) strips were used. In −UE strips, the UE was carefully excised with fine dissecting scissors as described elsewhere 3, 4, 8. All strips were mounted on a tissue myograph system (Danish Myo Technology Model 800MS, Aarhus, Denmark) containing carbogen‐aerated Krebs' solution at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Much effort is devoted to identify potential therapeutic targets pertaining to UE secretions that inhibit excessive bladder contraction. The presence of UE‐derived inhibitory diffusible factors is evidenced by enhanced detrusor smooth muscle contractility in UE‐denuded tissues 3–8. In mediating bladder relaxation, the roles of cyclic nucleotides 1, 9–11.…”

Section: Introductionmentioning

confidence: 99%