The role of Twist1 in hepatocellular carcinoma angiogenesis: a clinical study

Che, Na; Zhao, Xuyao; Sun, Tao; Zhao, Xingdong; Gu, Qing; Dong, Xue; Zhao, Nan; Liu, Yan Rong; Yao, Zhi; Sun, Bei

doi:10.1016/j.humpath.2010.03.010

Cited by 25 publications

(19 citation statements)

References 39 publications

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“…It is known that TWIST1 can stimulate angiogenesis feasibly through the up-regulation of MMPs (mostly MMP-9), Vimentin and N-cadherin5455. Unfortunately, comparing with TWIST1 and SNAIL1, the functional characteristics of SLUG have been less studied.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of EMT-inducing Transcription Factors (EMT-TFs) in Metastatic Breast Cancer: A Systematic Review and Meta-analysis

Imani

Hosseinifard

Cheng

et al. 2016

Sci Rep

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The epithelial-to-mesenchymal transition (EMT) is a vital control point in metastatic breast cancer (MBC). TWIST1, SNAIL1, SLUG, and ZEB1, as key EMT-inducing transcription factors (EMT-TFs), are involved in MBC through different signaling cascades. This updated meta-analysis was conducted to assess the correlation between the expression of EMT-TFs and prognostic value in MBC patients. A total of 3,218 MBC patients from fourteen eligible studies were evaluated. The pooled hazard ratios (HR) for EMT-TFs suggested that high EMT-TF expression was significantly associated with poor prognosis in MBC patients (HRs = 1.72; 95% confidence intervals (CIs) = 1.53–1.93; P = 0.001). In addition, the overexpression of SLUG was the most impactful on the risk of MBC compared with TWIST1 and SNAIL1, which sponsored fixed models. Strikingly, the increased risk of MBC was less associated with ZEB1 expression. However, the EMT-TF expression levels significantly increased the risk of MBC in the Asian population (HR = 2.11, 95% CI = 1.70–2.62) without any publication bias (t = 1.70, P = 0.11). These findings suggest that the overexpression of potentially TWIST1, SNAIL1 and especially SLUG play a key role in the aggregation of MBC treatment as well as in the improvement of follow-up plans in Asian MBC patients.

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Section: Discussionmentioning

confidence: 99%

Prognostic Value of EMT-inducing Transcription Factors (EMT-TFs) in Metastatic Breast Cancer: A Systematic Review and Meta-analysis

Imani

Hosseinifard

Cheng

et al. 2016

Sci Rep

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“…In the tumor stroma, blood vessels are induced from pre-existing blood vessels [21] or from circulating monocytes [22] due to activation and migration of endothelial cells by angiogenic growth factors, such as vascular endothelial growth factor or basic fibroblast growth factor, which are produced and released from tumor cells and stromal cells, as well as from endothelial precursor cells, followed by their directional migration towards the tumor and tube formation with extracellular matrix remodeling [23]. In order to characterize neoplastic angiogenesis in tissue samples, most investigations have focused on the number of microvessels in the lesion, expressed as microvessel density (MVD) usually through immunohistochemical detection of endothelial cell-specific antigens such as CD31, CD34, von Willebrand factor, and CD105 [14,17,[24][25][26]. MVD is positively correlated with metastasis and clinical behavior of cancer [27], although the relationship between angiogenesis and prognosis in head and neck cancers remains controversial [26,28,29].…”

Section: Discussionmentioning

confidence: 99%

“…In oral cancer, angiogenesis in the neoplastic stroma has been considered particularly important in characterizing individual cases, as neovascularization is one of the critical events that determines tumor growth and metastasis and has been regarded as a prognostic factor [14,15]. As a measure of angiogenesis in histological sections, the distribution of microvessel density (MVD) has been widely used [16,17]. On the other hand, microvascular architecture in the pre-invasive stages of oral mucosal neoplasia has not yet been investigated nor attracted any attention, though some devices, such as narrow-band imaging (NBI), to visualize abnormal microvascular patterns in mucosal neoplasms, have entered into practical use [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Intraepithelially entrapped blood vessels in oral carcinoma in-situ

et al. 2012

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It can be difficult to make a certain diagnosis in case of an oral borderline malignant lesion on hematoxylin-eosin-stained sections only. Furthermore, assessment of surgical margins of borderline lesions is difficult with the naked eye. We set out to determine the topographical distribution of capillary blood vessels within the epithelial zone and to assess its use as an aid for histopathological diagnosis and a framework for clinical assessment of lesional margins using optical techniques, such as narrow-band imaging (NBI) endoscopy. Capillary blood vessels entrapped in the epithelial compartment, which we have designated as intraepithelially entrapped blood vessels (IEBVs), were examined for their frequency, location, and shape in normal mucosa, dysplasia, and carcinoma in-situ (CIS) of the tongue using immunohistochemistry for CD31 and type IV collagen. When counted per unit length of epithelial surface, IEBVs increased in number significantly in CIS (5.6 ± 2.8), which was two times more than in normal (1.9 ± 1.6) and dysplastic (2.4 ± 1.5) epithelia. In addition, IEBVs in CIS had compressed shapes with occasional obstruction or collapse with hemorrhage and were arranged perpendicular to and extending up to the epithelial surface. These characteristic IEBVs in CIS were considered to be generated by complex expansion of rete ridges due to carcinoma cell proliferation within the limited epithelial space determined by the basement membrane. The recognition of IEBVs was helpful in the differential diagnosis of oral CIS, and the present data provide a valuable frame of reference for detecting oral CIS areas using such NBI-based optical devices.

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“…The negative staining control for each section was incubated in PBS containing 1 % normal goat serum without primary antibody. PV-9000 two-step plus Poly-HRP Anti-Mouse/Rabbit IgG Detection System (Zhongshan Chemical Co., Beijing, China) was used in this study according to the manufacturer's instruction [26]. The evaluation of the results was independently carried out by two authors (Jiye Zhu and Shan Huang) and an experienced pathologist (Junqi Huang) from When any disagreement about the results arose, three investigators re-examined the slides and discussed the results until a consensus was achieved.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%