The role of ultrasonographically guided puncture of the human rete testis in the therapeutic management of nonobstructive azoospermia*

Kaponis, Apostolos; Yiannakis, Dennis; Tsoukanelis, K.; Tsalikis, D.; Tsabalas, D.; Baltogiannis, D.; Giannakopoulos, X; Schrader, Mark; Georgiou, Ioannis; Yamamoto, Yasuhisa; Kanakas, N.; Miyagawa, Ikuo; Loutradis, Dimitrios; Touloupidis, Stavros; Sofikitis, Nikolaos

doi:10.1046/j.1439-0272.2003.00526.x

Cited by 6 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even in azoospermic patients, methods have been described to maximize the sperm extraction result with minimal testicular damage including testicular microdissection and ultrasonographically guided puncture of the rete testis [10] . Such a strategy will ensure the ability of these patients to father their own biological children by means of assisted reproductive techniques, in case they remain permanently azoospermic after the chemotherapeutic treatment.…”

Section: Discussionmentioning

confidence: 99%

Gynecomastia as a First Clinical Sign of Nonseminomatous Germ Cell Tumor

et al. 2011

Self Cite

View full text Add to dashboard Cite

Introduction: Gynecomastia is a common clinical sign in several diseases. In this report we present a case of gynecomastia with underlying testicular tumor which remained misdiagnosed for a prolonged period of time. Case Report: A 16-year-old adolescent noticed unilateral painless swelling of the left breast. He was referred to the Department of General Surgery and examined by a breast surgeon. A diagnosis of mastitis was made and a treatment with an oral antibiotic drug began. After failure of the initial antibiotic treatment, the patient was referred to the Department of Endocrinology and left testicular cancer was diagnosed. Unilateral high inguinal orchidectomy and subsequent chemotherapeutic treatment were performed. Conclusion: Primary care physicians should be aware of the possibility of a concomitant presence of gynecomastia and testicular cancer. We suggest a physical examination as well as a laboratory investigation, and testicular ultrasonography of the testes in all patients with gynecomastia.

show abstract

Section: Discussionmentioning

confidence: 99%

Gynecomastia as a First Clinical Sign of Nonseminomatous Germ Cell Tumor

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

Derivation of male germ cells from ram bone marrow mesenchymal stem cells by three different methods and evaluation of their fate after transplantation into the testis

Ghasemzadeh-Hasankolaei

Eslaminejad

Sedighi-Gilani

2015

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have the capacity to differentiate into germ cells (GCs). This research, for the first time, has evaluated the fate of in vitro MSC-derived GCs generated by three different induction methods and compared them after transplantation into the testes of rams. Passage-3 ram bone marrow (BM)-MSCs were divided into three treatment groups: (1) 14-d treatment with 10 μM retinoic acid (RA; RA14), (2) 21-d treatment with 10 μM RA (RA21), and (3) 21-d treatment with 10 ng/ml transforming growth factor beta-1 (TGFb1). After confirmation of the existence of germ-like cells in the culture, the treated cells were labeled and transplanted into the testes of ram lambs. After 2 mo, we conducted histological evaluations of the rams' testes. Results showed that in vitro-derived GCs from all treatment groups survived in the testes. Some of these GCs homed at the basement membrane of seminiferous tubules and formed colonies. The homed cells and cell colonies were similar to testicular native spermatogonia and expressed PGP9.5. TGFb1 exhibited the highest efficiency for in vitro production of GCs as well as the highest capability for homing and colony formation in the testes. RA21 was less efficient than TGFb1, particularly in colony formation. RA14 was the weakest group. No further differentiation of the transplanted GCs was observed. From our results, it could be concluded that a 21-d treatment period of BM-MSCs with TGFb1 is the most efficient method for in vitro generation of spermatogonia-like cells that survive, home, and form colonies in the testes.

show abstract