The role of upregulated miR-375 expression in breast cancer: An in vitro and in silico study

Tang, Wei; Li, Guosheng; Li, Jian‐Di; Pan, Wenya; Shi, Qi; Xiong, Dandan; Mo, Chao-Hua; Zeng, Jingjing; Chen, Gang; Huang, Su-Hua; Rong, Minhua

doi:10.1016/j.prp.2019.152754

Cited by 33 publications

(26 citation statements)

References 73 publications

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“…MiR‐375 plays a dual role in the development and progression of cancer. It acts as an oncomiR in some cancers 29,30 but as a tumor suppressor miRNA in the others 31,32 . A high frequency of recurrence and poor survival is observed in GC patients with high level of miR‐375 33 .…”

Section: Discussionmentioning

confidence: 99%

CircHN1 affects cell proliferation and migration in gastric cancer

Zhang

Wang

Zang

et al. 2020

Clinical Laboratory Analysis

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Background Increasing evidence indicates that circular RNAs (circRNAs) are dysregulated in human cancers. The biological roles of circRNAs in gastric cancer (GC) have not been well‐characterized. Methods The GEO database was used to analyze circRNA expression profile in GC. The expression level of target circRNA in tumor tissues and adjacent non‐tumor tissues was detected by reverse transcription‐quantitative PCR. Gene transfection was used to manipulate the expression of circRNAs. The biological roles of circRNAs in cell proliferation, migration, and invasion were determined by cell counting, colony formation, transwell migration, Matrigel invasion, and mouse xenograft tumor assays. The interactions between circRNAs and miRNAs were verified by RNA immunoprecipitation and luciferase reporter assays. Results We found that circHN1 was upregulated in GC tissues and cell lines compared to adjacent non‐tumor tissues and normal gastric epithelial cells. Additionally, circHN1 silencing significantly promoted GC cell growth, colony formation, migration, and invasion, whereas circHN1 overexpression had the opposite effects. CircHN1 overexpression also suppressed gastric cancer growth in the mouse xenograft tumor model. CircHN1 was mainly localized in the cytoplasm of GC cells and could bind to AGO2. MiR‐1248 and miR‐375 were predicted to interact with circHN1 by bioinformatic analyses. MiR‐1248 and miR‐375 overexpression inhibited the activity of the circHN1 luciferase reporter. Conclusion CircHN1 is aberrantly expressed in GC and affects the proliferation and migration of gastric cancer cells by acting as miRNA sponge.

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Section: Discussionmentioning

confidence: 99%

CircHN1 affects cell proliferation and migration in gastric cancer

Zhang

Wang

Zang

et al. 2020

Clinical Laboratory Analysis

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“…In addition, miR-375 has been associated with the prognosis of several types of cancer. For example, circulating miR-375 is associated with the outcome in treatment-resistant prostate cancer ( 45 ), although, to the best of our knowledge, the detailed mechanism has not been investigated, and the expression of miR-375 is associated with breast cancer prognosis ( 46 ). However, the role of miR-375 in glioma has not yet been defined.…”

Section: Discussionmentioning

confidence: 99%

lncRNA KCNQ1OT1 promotes proliferation and invasion of glioma cells by targeting the miR‑375/YAP pathway

et al. 2020

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The long non-coding RNA KCNQ1OT1 is generally recognized as an oncogenic molecule in several human malignant tumors. However, to the best of our knowledge, the role of KCNQ1OT1 in glioma has not been fully investigated. The current study aimed to probe the biological function of KCNQ1OT1 in human glioma cell lines and its mechanisms. The glioma cell lines U251 and U87-MG were used as cell models. Cell proliferation and apoptosis assays were used to measure the effects of different treatments on survival, and reverse transcription-quantitative PCR and western blot-ting were used to investigate the expression profiles of key molecules. Migration and invasion assays were conducted to reveal the biological features of glioma cells. The results indicated that KCNQ1OT1 was upregulated in glioma tissues compared with adjacent tissues, which was associated with poor prognosis. Additionally, knockdown of KCNQ1OT1 in U251 and U87-MG cells inhibited cell proliferation, migration and invasion, but had no effect on apoptosis. The effects of KCNQ1OT1 on migration and invasion were partially attributed to enhanced Yes-associated protein (YAP) expression levels and epithelial-mesenchymal transition (EMT) signaling. Furthermore, microRNA (miR)-375 functioned as a link between KCNQ1OT1 and YAP in regulating cell proliferation. Finally, the KCNQ1OT1/miR-375/YAP axis modulated cell proliferation and cell fate by affecting the modulated YAP-mediated EMT signaling. In conclusion, the KCNQ1OT1/miR-375/YAP axis modulated migration and invasion of glioma cells by affecting EMT signaling; thus, targeting KCNQ1OT1 may represent a promising strategy in glioma therapeutics.

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“…Another target gene, CREB1 and PRKACA show also negative correlation with miR-1275 level. Whereas CREB1 was found to reduce apoptosis process and increase cell proliferation in breast cancer (43), PRKACA plays a key role in tumorigenesis and development of BC (44). However, the role of THSD4 and SYT7, currently unidenti ed, may enhance tumor growth in a variety of cancers, especially breast cancer (45,46).…”

Section: Discussionmentioning

confidence: 99%

Identification of Tumor-Suppressive miRNA-1275 as a Novel Marker for Breast Cancer (BC) by MACE-Sequencing and RT-qPCR Techniques

Majed

Mustafa

2020

Preprint

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IntroductionDisruption of cellular processes in the breast by abnormally expressed miRNA is characterized to develop cancer. We aimed to determine the differential expression of coding and non-coding RNAs in formalin fixed paraffin embedded (FFPE) blocks of breast cancer (BC) tissue and normal adjacent tissue (NAT). Another aim is to determine differential expression of has-miR-1275 as novel biomarker for BC and identify its target genes using prediction sites and experimentally expression level of them via the MACE-sequencing technique. MethodsMACE-sequencing technique was utilized to analyze differential expression of coding RNAs and small RNAs (sRNAs). Among small RNAs, miRNA-1275 expression was focused and confirmed using RT-qPCR technique in 20 Kurdish cases with BC . Moreover, clinical significance of miR- 1275 and its target genes was studied in a large number of patients with BC using the data obtained from The Cancer Genome Atlas database.ResultsThe MACE-seq findings showed that 1400 sRNAs and 26843 coding RNAs were differentially expressed in FFPE of BC tissue compared to NAT. Among these sRNAs, miRNA-1275 expression was found to be decreased in BC tissue compared to NAT. The decreased expression level of which was then confirmed via RT-qPCR technique to farther prove in 20 Kurdish cases with BC . Furthermore, the correlation between the expression level of miRNA-1275 and clinical data were evaluated to be highly corrected in cases with BC (overall survival rate: P = 0.0401). However, putative target genes ( DVL3, PPP 2R2D, THSD4, CREB1, SYT7, and PRKACA) were computationally identified as direct targets of miRNA-1275 in several target predicted sites. Among coding RNAs, the expression level of these targets was increased in BC tissue compared to NAT. The levels of these targets were negatively associated with miRNA-1275 expression. Finally, the role of down-expressed miRNA-1275 and its targets in BC cells were identified to attenuated biological mechanisms; including cell growth, proliferation, movement, invasion, metastasis, and apoptosis.Conclusiondown-expressed miR-1275 , a tumor suppressor, is as a novel biomarker for early detection of breast cancer. DVL3, PPP 2R2D, THSD4, CREB1, SYT7, and PRKACA are novely identified to be targeted by miR-1275 in BC cells.

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The role of upregulated miR-375 expression in breast cancer: An in vitro and in silico study

Cited by 33 publications

References 73 publications

CircHN1 affects cell proliferation and migration in gastric cancer

CircHN1 affects cell proliferation and migration in gastric cancer

lncRNA KCNQ1OT1 promotes proliferation and invasion of glioma cells by targeting the miR‑375/YAP pathway

Identification of Tumor-Suppressive miRNA-1275 as a Novel Marker for Breast Cancer (BC) by MACE-Sequencing and RT-qPCR Techniques

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