The role of vimentin in directional migration of rat fibroblasts

Vakhrusheva, Anna; Endzhievskaya, Sofia; Жуйков, В. А.; Nekrasova, Tatyana; Parshina, E. Yu.; Ovsiannikova, Natalia L.; Попов, В. С.; Bagrov, D. V.; Минин, А. А.; Sokolova, Olga

doi:10.1002/cm.21572

Cited by 18 publications

(10 citation statements)

References 42 publications

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“…VIM has been implicated in two major cellular functions: mesenchymal cell motility and preservation of nuclear-cytoskeletal integrity, especially in response to compressive stress. It has also been found to play an important role in cell spreading on firm 2D substrates [62][63][64][65][66][67][68][69][70].…”

Section: Cells In the Pacc State Have Increased Vimentin Expressionmentioning

confidence: 99%

Cells in the Polyaneuploid Cancer Cell (PACC) state have increased metastatic potential

Mallin

Kim

Choudhury

et al. 2022

Preprint

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Although metastasis is the leading cause of cancer death, it is quite rare at the cellular level. Only a rare subset of cancer cells (~1 in 1.5 billion) can complete the entire metastatic cascade: invasion, intravasation, survival in the circulation, extravasation, and colonization (i.e. are metastasis competent). We propose that cells engaging a Polyaneuploid Cancer Cell (PACC) phenotype are metastasis competent. PACCs are enlarged, non-dividing cells with increased genomic content that form in response to stress. Single-cell tracking using time-lapse microscopy reveals that PACCs are more motile than nonPACCs. Additionally, PACCs exhibit increased capacity for environment-sensing and directional migration in chemotactic environments, predicting successful invasion. Magnetic Twisting Cytometry and Atomic Force Microscopy reveal that cells in the PACC state display hyper-elastic properties like increased peripheral deformability and maintained peri-nuclear cortical integrity that predict successful intravasation and extravasation. Furthermore, four orthogonal methods reveal that PACCs have increased expression of Vimentin, a known hyper-elastic biomolecule. Lastly, anoikis-resistance assays and detection of PACCs in the blood of a patient with metastatic castrate-resistant prostate cancer using a selection-free circulating tumor cell detection platform reveal that PACCs are capable of surviving in the circulation. Taken together with the knowledge that PACCs are capable of eventual depolyploidization and progeny formation (as a potential route to colonization), these data support PACCs as candidate metastasis-competent cells worthy of further analysis.

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Section: Cells In the Pacc State Have Increased Vimentin Expressionmentioning

confidence: 99%

Cells in the Polyaneuploid Cancer Cell (PACC) state have increased metastatic potential

Mallin

Kim

Choudhury

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…As a result, vimentin not only enhances cell integrity after exposure to shear force, but also provides flexibility in cells recovering from membrane deformation. The fine tuning of membrane stiffness by vimentin may contribute to a wide array of biological functions including cell migration, division, cell adhesion to substrates, autophagy, and signal transduction (Wang and Stamenovic, 2002;Vakhrusheva et al, 2019). Cell migration through ECMs like collagen networks often provokes nuclear rupture, which is related to constriction and deformation of the nuclear membrane as cells traverse pores in the ECM.…”

Section: Viscoelastic Properties Of Vimentin Enhance Cell Resistance ...mentioning

confidence: 99%

Impact of Vimentin on Regulation of Cell Signaling and Matrix Remodeling

Ostrowska-Podhorodecka

Ding

Norouzi

et al. 2022

Front. Cell Dev. Biol.

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Vimentin expression contributes to cellular mechanoprotection and is a widely recognized marker of fibroblasts and of epithelial-mesenchymal transition. But it is not understood how vimentin affects signaling that controls cell migration and extracellular matrix (ECM) remodeling. Recent data indicate that vimentin controls collagen deposition and ECM structure by regulating contractile force application to the ECM and through post-transcriptional regulation of ECM related genes. Binding of cells to the ECM promotes the association of vimentin with cytoplasmic domains of adhesion receptors such as integrins. After initial adhesion, cell-generated, myosin-dependent forces and signals that impact vimentin structure can affect cell migration. Post-translational modifications of vimentin determine its adaptor functions, including binding to cell adhesion proteins like paxillin and talin. Accordingly, vimentin regulates the growth, maturation and adhesive strength of integrin-dependent adhesions, which enables cells to tune their attachment to collagen, regulate the formation of cell extensions and control cell migration through connective tissues. Thus, vimentin tunes signaling cascades that regulate cell migration and ECM remodeling. Here we consider how specific properties of vimentin serve to control cell attachment to the underlying ECM and to regulate mesenchymal cell migration and remodeling of the ECM by resident fibroblasts.

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“…Vimentin intermediate filaments are expressed in endothelial and mesenchymal cells and these filaments are known as a marker of HGFs. Vimentin is a major type III intermediate filament protein that plays a key role in cell adhesion, migration, signal transduction, neurite extension, and angiogenesis 76 77 .…”

Section: Discussionmentioning

confidence: 99%

Effects of titanium dioxide microparticles and nanoparticles on cytoskeletal organization, cell adhesion, migration, and proliferation in human gingival fibroblasts in the presence of lipopolysaccharide

Sun

et al. 2022

J of Periodontal Research

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Background and objective: Titanium wear particles may participate in the etiology of peri-implantitis. However, the influence of titanium wear particles on biological behavior of human gingival fibroblasts (HGFs) in the presence of LPS is still not clear.The present study demonstrated the effects of titanium dioxide micro-and nanoparticles (TiO 2 MPs and NPs) on HGF cell viability, cytoskeletal organization, adhesion, migration, and proliferation in vitro, and LPS was used to mimic the in vivo condition.Methods: Primary HGFs were treated with TiO 2 MPs (primary particle size <5 μm, 0.1 mg/ml) and NPs (primary particle size <100 nm, 0.1 mg/ml) with or without 1 μg/ ml LPS. The effects of TiO 2 MPs and NPs on HGFs cell viability was measured by CCK-8 assay. The proliferation of HGF was detected by Ki67 nuclear staining. The confocal laser scanning microscope (CLSM) was used to detect the internalization of TiO 2 MPs and NPs in HGFs as well as the arrangement of F-actin, vinculin, and vimentin organization. Wound healing assay and transwell assay were performed to measure the migration of HGFs induced by TiO 2 MPs and NPs. Cell adhesion was measured using fibronectin-coated plates. The relative mRNA and protein expression of adhesion relative protein such as focal adhesion kinase (FAK), fibronectin (FN), and type I collagen (COL1) were measured using quantitative RT-PCR and western blot analysis. One-way analysis of variance (ANOVA) and Student's t-test were used to analyze the statistical significance, and p < .05 was considered statistically significant.Results: TiO 2 NPs significantly inhibited HGF cell viability, proliferation, and migration compared with TiO 2 MPs group and control group. Compared with control group (2.64 ± 0.09), the mean absorbance of the cells in 1 mg/ml TiO 2 MPs group and 0.25 mg/ml TiO 2 NPs group were significantly decreased to 1.93 ± 0.33 (p < .05) and 2.22 ± 0.18 (p < .01), respectively. The cytoskeleton disruption was found in TiO 2 NPs group. The mRNA and protein expression were significantly downregulated by TiO 2 NPs. Furthermore, both TiO 2 NPs and MPs induced more adverse effects on HGFs in the presence of LPS.

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The role of vimentin in directional migration of rat fibroblasts

Cited by 18 publications

References 42 publications

Cells in the Polyaneuploid Cancer Cell (PACC) state have increased metastatic potential

Cells in the Polyaneuploid Cancer Cell (PACC) state have increased metastatic potential

Impact of Vimentin on Regulation of Cell Signaling and Matrix Remodeling

Effects of titanium dioxide microparticles and nanoparticles on cytoskeletal organization, cell adhesion, migration, and proliferation in human gingival fibroblasts in the presence of lipopolysaccharide

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