The role of Wg signaling in the patterning of embryonic leg primordium in Drosophila

Kubota, Kazuhiko; Goto, Satoshi; Hayashi, Shigeo

doi:10.1016/s0012-1606(03)00062-9

Cited by 32 publications

(43 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…intact, as shown by the presence of the coxa-specific bristles cx-1 and cx-2 (Grossmann et al, 2009). Thus, Tc-fz1-dependent Wnt signalling is crucial for appendage elongation, as was previously reported for wingless in Drosophila (Kubota et al, 2003) and Tribolium (Grossmann et al, 2009).…”

Section: (S) Tc-fz1supporting

confidence: 76%

A context-dependent combination of Wnt receptors controls axis elongation and leg development in a short germ insect

et al. 2011

View full text Add to dashboard Cite

SUMMARYShort germ embryos elongate their primary body axis by consecutively adding segments from a posteriorly located growth zone. Wnt signalling is required for axis elongation in short germ arthropods, including Tribolium castaneum, but the precise functions of the different Wnt receptors involved in this process are unclear. We analysed the individual and combinatorial functions of the three Wnt receptors, Frizzled-1 (Tc-Fz1), Frizzled-2 (Tc-Fz2) and Frizzled-4 (Tc-Fz4), and their co-receptor Arrow (Tc-Arr) in the beetle Tribolium. Knockdown of gene function and expression analyses revealed that Frizzled-dependent Wnt signalling occurs anteriorly in the growth zone in the presegmental region (PSR). We show that simultaneous functional knockdown of the Wnt receptors Tc-fz1 and Tc-fz2 via RNAi resulted in collapse of the growth zone and impairment of embryonic axis elongation. Although posterior cells of the growth zone were not completely abolished, Wnt signalling within the PSR controls axial elongation at the level of pair-rule patterning, Wnt5 signalling and FGF signalling. These results identify the PSR in Tribolium as an integral tissue required for the axial elongation process, reminiscent of the presomitic mesoderm in vertebrates. Knockdown of Tc-fz1 alone interfered with the formation of the proximo-distal and the dorso-ventral axes during leg development, whereas no effect was observed with single Tc-fz2 or Tc-fz4 RNAi knockdowns. We identify Tc-Arr as an obligatory Wnt co-receptor for axis elongation, leg distalisation and segmentation. We discuss how Wnt signalling is regulated at the receptor and co-receptor levels in a dose-dependent fashion.

show abstract

Section: (S) Tc-fz1supporting

confidence: 76%

A context-dependent combination of Wnt receptors controls axis elongation and leg development in a short germ insect

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Both a barrier to diffusion of the ligands and downstream repression could be overcome if the amount of ligand or downstream signalling is experimentally elevated. Suppression of the Wg and Egfr signalling pathways in late embryonic development, in addition, is a necessary step for Development 130 (26) Research article the specification of the wing disc primordium and its segregation from the leg disc (Kubota et al, 2000;Kubota et al, 2003). It seems possible, therefore, that the repression of these signalling pathways, inherited from the embryo, is later restricted to the peripodial side of the disc; alternatively, the repression of Wg and Egfr signalling in the PE might arise later as a non-related event.…”

Section: Discussionmentioning

confidence: 99%

Wg and Egfr signalling antagonise the development of the peripodial epithelium inDrosophilawing discs

2003

View full text Add to dashboard Cite

Imaginal discs contain a population of cells, known as peripodial epithelium, that differ morphologically and genetically from the rest of imaginal cells. The peripodial epithelium has a small contribution to the adult epidermis, though it is essential for the eversion of the discs during metamorphosis. The genetic mechanisms that control the identity and cellular morphology of the peripodial epithelia are poorly understood. In this report,we investigate the mechanisms that pattern the peripodial side of the wing imaginal disc during early larval development. At this time, the activities of the Wingless (Wg) and Epidermal growth factor receptor (Egfr) signalling pathways specify the prospective wing and notum fields, respectively. We show that peripodial epithelium specification occurs in the absence of Wingless and Egfr signalling. The ectopic activity in the peripodial epithelium of any of these signalling pathways transforms the shape of peripodial cells from squamous to columnar and resets their gene expression profile. Furthermore,peripodial cells where Wingless signalling is ectopically active acquire hinge identity, while ectopic Egfr activation results in notum specification. These findings suggest that suppression of Wg and Egfr activities is an early step in the development of the peripodial epithelium of the wing discs.

show abstract

“…In Drosophila melanogaster imaginal discs, a number of signalling molecules are required for the induction of Dll expression. Dll is regulated by gene products like Wingless (Wg), Ultrabithorax (Ubx), BMP, Decapentaplegic (dpp) and the EGF pathway (Cohen 1990;Vachon et al 1992;Kubota et al 2000Kubota et al , 2003. In Drosophila embryos, the initial expression of en in response to Fushi tarazu (Ftz) and Even-skipped (Eve) is replaced after a short period by an autoregulatory loop in which secretion of Wg protein from the adjacent cells is needed for expression of en; and expression of en is needed for expression of wg (Vincent and O'Farrell 1992;Vincent and Lawrence 1994).…”

Section: Expression Of Dll In the Amphipod And Isopod Germ Bandsmentioning

confidence: 99%

Clonal analysis of Distal-less and engrailed expression patterns during early morphogenesis of uniramous and biramous crustacean limbs

Hejnol

Scholtz

2004

Dev Genes Evol

View full text Add to dashboard Cite

In order to investigate the correlation of cell lineage, gene expression, and morphogenesis of uniramous and biramous limbs we studied limb formation in the thorax and pleon of the amphipod Orchestia cavimana and the isopod Porcellio scaber. We took advantage of the fact that in amphipod and isopod crustaceans--both Malacostraca--uniramous limbs evolved independently in the thorax whereas ancestral biramous limbs are formed in the pleon (abdomen). The gene Distal-less is expressed in the early limb buds as in other arthropods. Accordingly, it is likely to be responsible for the development of the proximodistal axis of the appendages. Double staining of Distal-less and Engrailed proteins suggests that Distal-less in the pleon of the amphipod Orchestia might not be under the control of the Wingless protein. Additionally, we studied axis formation of the uniramous and biramous limbs. In both species investigated, biramous limbs originate exclusively by the subdivision of the original limb bud. Both distal elements continuously express Distal-less. There is flexibility in the suppression of the development of additional branches in the crustacean limb. In the amphipod O. cavimana, uniramous thoracopods are formed by downregulation of Distal-less in the area where, in biramous limbs, the exopodites would occur. In contrast, this region never expresses Distal-less in the uniramous thoracopods of the isopod P. scaber. Our results suggest that the gene expression pattern is independent of the cell division pattern. Gene expression domains and morphogenesis of limbs and segments, on the other hand, show a good correlation.

show abstract

The role of Wg signaling in the patterning of embryonic leg primordium in Drosophila

Cited by 32 publications

References 37 publications

A context-dependent combination of Wnt receptors controls axis elongation and leg development in a short germ insect

A context-dependent combination of Wnt receptors controls axis elongation and leg development in a short germ insect

Wg and Egfr signalling antagonise the development of the peripodial epithelium inDrosophilawing discs

Clonal analysis of Distal-less and engrailed expression patterns during early morphogenesis of uniramous and biramous crustacean limbs

Contact Info

Product

Resources

About