Wg and Egfr signalling antagonise the development of the peripodial epithelium in<i>Drosophila</i>wing discs

Baena‐López, Luis Alberto; Pastor-Pareja, José Carlos; Resino, Jaime

doi:10.1242/dev.00884

Cited by 25 publications

(21 citation statements)

References 44 publications

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“…Together the data support the idea that CG4382 functions as a negative regulator of vein development, which is repressed by a high level of Egfr signaling. The expression of CG4382 in the peripodial membrane overlying the disc proper suggests that its function in venation appears to involve communication between two cell layers, as has been noted for patterning of the major regions of the wing disc (Baena-Lopez et al 2003;Pallavi and Shashidhara 2003). Our results suggest the peripodial membrane may also have a more specific role in vein patterning.…”

Section: Cg6234supporting

confidence: 74%

New Negative Feedback Regulators of Egfr Signaling inDrosophila

et al. 2012

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The highly conserved epidermal growth factor receptor (Egfr) pathway is required in all animals for normal development and homeostasis; consequently, aberrant Egfr signaling is implicated in a number of diseases. Genetic analysis of Drosophila melanogaster Egfr has contributed significantly to understanding this conserved pathway and led to the discovery of new components and targets. Here we used microarray analysis of third instar wing discs, in which Egfr signaling was perturbed, to identify new Egfrresponsive genes. Upregulated transcripts included five known targets, suggesting the approach was valid. We investigated the function of 29 previously uncharacterized genes, which had pronounced responses. The Egfr pathway is important for wing-vein patterning and using reverse genetic analysis we identified five genes that showed venation defects. Three of these genes are expressed in vein primordia and all showed transcriptional changes in response to altered Egfr activity consistent with being targets of the pathway. Genetic interactions with Egfr further linked two of the genes, Sulfated (Sulf1), an endosulfatase gene, and CG4096, an A Disintegrin And Metalloproteinase with ThromboSpondin motifs (ADAMTS) gene, to the pathway. Sulf1 showed a strong genetic interaction with the neuregulin-like ligand vein (vn) and may influence binding of Vn to heparan-sulfated proteoglycans (HSPGs). How Drosophila Egfr activity is modulated by CG4096 is unknown, but interestingly vertebrate EGF ligands are regulated by a related ADAMTS protein. We suggest Sulf1 and CG4096 are negative feedback regulators of Egfr signaling that function in the extracellular space to influence ligand activity.

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Section: Cg6234supporting

confidence: 74%

New Negative Feedback Regulators of Egfr Signaling inDrosophila

et al. 2012

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“…It has been shown earlier that repression of WG early during development is a prerequisite to specify peripodial fate (Baena-Lopez et al, 2003). It is possible that the mechanism operating in PM cells to repress WG is downstream of the N signaling pathway.…”

Section: Overexpression Of Delta In Pm Activates Wingless Expression mentioning

confidence: 98%

“…However, peripodial epithelium does not express WG or VG, which are required for the specification of the wing pouch, nor does it express Iro-C complex genes (Baena-Lopez et al, 2003), which specify notum identity. Nevertheless, PM cells show similarity to DP cells at the levels of patterning events along the AP axis.…”

Section: Apical-basal Polarity In Pm Cellsmentioning

confidence: 99%

“…However, elucidation of these cell-cell interactions is entirely related to events in the disc proper, whereas patterning events in PM are not well understood. Possible differences between PM and DP in these events are indicated in the reports that DPP diffused from the DP acts as a survival signal for PM cells (Gibson et al, 2002) and suppression of WG and epidermal growth factor receptor signaling at early stages of wing specification helps PM cells to acquire squamous morphology (Baena-Lopez et al, 2003). The complete absence of Apterous (AP) and WG expression in PM (Pallavi and Shashidhara, 2003) suggests ventral identity to all PM cells.…”

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confidence: 99%

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Signaling interactions between squamous and columnar epithelia of the Drosophila wing disc

Pallavi

Shashidhara

2005

Journal of Cell Science

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Understanding the interactions between distinct epithelial cells would help us to understand the development of tissues. Drosophila imaginal discs, which are made up of two types of epithelial cells, provide good model systems for such studies. The disc proper or the columnar epithelial cells are apposed to a layer of squamous epithelial cells (the peripodial membrane). We have examined organization of peripodial and disc proper cells vis-à-vis their polarity since cell polarity plays an important role in the polarized transport of signaling molecules. With the help of polarity-specific cell markers, we have observed that apical surfaces of peripodial and disc proper cells face each other. This provides the cellular basis for the recently demonstrated signaling interactions between peripodial and disc proper cells during disc patterning. We also report significant similarities as well as differences between peripodial and disc proper cells in Engrailed-dependent wingdisc-patterning events, which make them an appropriate model system for studying the mechanism of diffusion of signal molecules, such as Hedgehog. Results with wild-type and two mutant forms of Hedgehog suggest that direct cell-cell contact is a requirement for the movement of wild-type Hedgehog signal and reconfirm that cholesterol-modification of Hedgehog makes it a short-range signaling molecule by restricting its movement.

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“…1A). Cuboidal cells of both layers have been termed 'medial edge', 'cubic' and 'margin' cells, we use margin cells because of its original use (Baena-Lopez et al, 2003;Pallavi and Shashidhara, 2003;Zeitlinger and Bohmann, 1999) (Fig. 1B,C).…”

Section: Morphogenesis Of the Pe In Different Discsmentioning

confidence: 99%

Developmental analysis and squamous morphogenesis of the peripodial epithelium in Drosophila imaginal discs

McClure

Schubiger

2005

Development

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Imaginal discs of Drosophila provide an excellent system with which to study morphogenesis, pattern formation and cell proliferation in an epithelium. Discs are sac-like in structure and are composed of two epithelial layers: an upper peripodial epithelium and lower disc proper. Although development of the disc proper has been studied extensively in terms of cell proliferation, cell signaling mechanisms and pattern formation, little is known about these same processes in the peripodial epithelium. We address this topic by focusing on morphogenesis, compartmental organization, proliferation and cell lineage of the PE in wing, second thoracic leg (T2) and eye discs. We show that a subset of peripodial cells in different imaginal discs undergo a cuboidal-to-squamous cell shape change at distinct larval stages. We find that this shape change requires both Hedgehog and Decapentapelagic, but not Wingless, signaling. Additionally, squamous morphogenesis shifts the anteroposterior (AP) compartment boundary in the peripodial epithelium relative to the stationary AP boundary in the disc proper. Finally, by lineage tracing cells in the PE, we surprisingly find that peripodial cells are displaced into the disc proper during larval development and this movement leads to Ubx repression.

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Wg and Egfr signalling antagonise the development of the peripodial epithelium inDrosophilawing discs

Cited by 25 publications

References 44 publications

New Negative Feedback Regulators of Egfr Signaling inDrosophila

New Negative Feedback Regulators of Egfr Signaling inDrosophila

Signaling interactions between squamous and columnar epithelia of the Drosophila wing disc

Developmental analysis and squamous morphogenesis of the peripodial epithelium in Drosophila imaginal discs

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