Signaling interactions between squamous and columnar epithelia of the Drosophila wing disc

Pallavi, SK; Shashidhara, L.S.

doi:10.1242/jcs.02464

Cited by 28 publications

(36 citation statements)

References 37 publications

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“…S4A). This observation is similar to a previous report that ectopic expression of Delta in peripodial cells triggers Notch activation in disk-proper cells (27), and provides further support that direct cell-cell contact occurs between the apical surfaces of these two distinct epithelial cell types. In addition, we noticed a clear enrichment of the GFP ligand in puncta associated with cytoneme-like cell protrusions ( Fig.…”

Section: Resultssupporting

confidence: 91%

Development of an optimized synthetic Notch receptor as an in vivo cell–cell contact sensor

Huang

Perrimon

2017

Proc. Natl. Acad. Sci. U.S.A.

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Detection and manipulation of direct cell-cell contact in complex tissues is a fundamental and challenging problem in many biological studies. Here, we report an optimized Notch-based synthetic receptor (synNQ) useful to study direct cell-cell interactions in Drosophila. With the synNQ system, cells expressing a synthetic receptor, which contains Notch activation machinery and a downstream transcriptional activator, QF, are activated by a synthetic GFP ligand expressed by contacting neighbor cells. To avoid cis-inhibition, mutually exclusive expression of the synthetic ligand and receptor is achieved using the "flippase-out" system. Expression of the synthetic GFP ligand is controlled by the Gal4/UAS system for easy and broad applications. Using synNQ, we successfully visualized cell-cell interactions within and between most fly tissues, revealing previously undocumented cell-cell contacts. Importantly, in addition to detection of cells in contact with one another, synNQ allows for genetic manipulation in all cells in contact with a targeted cell population, which we demonstrate in the context of cell competition in developing wing disks. Altogether, the synNQ genetic system will enable a broad range of studies of cell contact in developmental biology.

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Section: Resultssupporting

confidence: 91%

Development of an optimized synthetic Notch receptor as an in vivo cell–cell contact sensor

Huang

Perrimon

2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Interestingly these two events, apical and basal expulsion, can occur side by side in the same region of a given disc. The cellular environment may play a role since apically-expulsed cells will find themselves closer in proximity to the peripodial membrane and will thereby be under the influence of signaling molecules like Dpp expressed by this tissue [32], [33].…”

Section: Discussionmentioning

confidence: 99%

Mutations in the Polycomb Group Gene polyhomeotic Lead to Epithelial Instability in both the Ovary and Wing Imaginal Disc in Drosophila

et al. 2010

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BackgroundMost human cancers originate from epithelial tissues and cell polarity and adhesion defects can lead to metastasis. The Polycomb-Group of chromatin factors were first characterized in Drosophila as repressors of homeotic genes during development, while studies in mammals indicate a conserved role in body plan organization, as well as an implication in other processes such as stem cell maintenance, cell proliferation, and tumorigenesis. We have analyzed the function of the Drosophila Polycomb-Group gene polyhomeotic in epithelial cells of two different organs, the ovary and the wing imaginal disc.ResultsClonal analysis of loss and gain of function of polyhomeotic resulted in segregation between mutant and wild-type cells in both the follicular and wing imaginal disc epithelia, without excessive cell proliferation. Both basal and apical expulsion of mutant cells was observed, the former characterized by specific reorganization of cell adhesion and polarity proteins, the latter by complete cytoplasmic diffusion of these proteins. Among several candidate target genes tested, only the homeotic gene Abdominal-B was a target of PH in both ovarian and wing disc cells. Although overexpression of Abdominal-B was sufficient to cause cell segregation in the wing disc, epistatic analysis indicated that the presence of Abdominal-B is not necessary for expulsion of polyhomeotic mutant epithelial cells suggesting that additional POLYHOMEOTIC targets are implicated in this phenomenon.ConclusionOur results indicate that polyhomeotic mutations have a direct effect on epithelial integrity that can be uncoupled from overproliferation. We show that cells in an epithelium expressing different levels of POLYHOMEOTIC sort out indicating differential adhesive properties between the cell populations. Interestingly, we found distinct modalities between apical and basal expulsion of ph mutant cells and further studies of this phenomenon should allow parallels to be made with the modified adhesive and polarity properties of different types of epithelial tumors.

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“…The EAD consist of a basal CE and an apical PE that face each other with their apical sides (Pallavi and Shashidhara, 2005) and are surrounded by the basal membrane. In addition, circulating blood cells, hemocytes, populate the outer surface of the EAD (Holz et al, 2003).…”

Section: Cher Accumulates In Invasive Clonal Tumorsmentioning

confidence: 99%

The actin cross-linker Filamin/Cheerio mediates tumor malignancy downstream of JNK signaling

Külshammer¹,

Uhlířová²

2012

Journal of Cell Science

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SummaryCell shape dynamics, motility, and cell proliferation all depend on the actin cytoskeleton. Malignant cancer cells hijack the actin network to grow and migrate to secondary sites. Understanding the function of actin regulators is therefore of major interest. In the present study, we identify the actin cross-linking protein Filamin/Cheerio (Cher) as a mediator of malignancy in genetically defined Drosophila tumors. We show that in invasive tumors, resulting from cooperation of activated Ras with disrupted epithelial cell polarity, Cher is upregulated in a Jun N-terminal kinase (JNK)-dependent manner. Although dispensable in normal epithelium, Cher becomes required in the tumor cells for their growth and invasiveness. When deprived of Cher, these tumor clones lose their full potential to proliferate and breach tissue boundaries. Instead, the Cher-deficient clones remain confined within the limits of their source epithelium, permitting survival of the host animal. Through interaction with the myosin II heavy chain subunit, Cher is likely to strengthen the cortical actomyosin network and reinforce mechanical tension within the invasive tumors. Accordingly, Cher is required for aberrant expression of genes downstream of the Hippo/Yorkie signaling in the tumor tissue. Our study identifies Cher as a new target of JNK signaling that links cytoskeleton dynamics to tumor progression.

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Signaling interactions between squamous and columnar epithelia of the Drosophila wing disc

Cited by 28 publications

References 37 publications

Development of an optimized synthetic Notch receptor as an in vivo cell–cell contact sensor

Development of an optimized synthetic Notch receptor as an in vivo cell–cell contact sensor

Mutations in the Polycomb Group Gene polyhomeotic Lead to Epithelial Instability in both the Ovary and Wing Imaginal Disc in Drosophila

The actin cross-linker Filamin/Cheerio mediates tumor malignancy downstream of JNK signaling

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