The roles of hydrophobic group on the surface of ultra low dielectric constant porous silica film during thermal treatment

Luo, Jen-Tsung; Wu, Wen−Fa; Wen, Hua-Chiang; Wan, Ben‐Zu; Chang, Yuan-Hsiou; Chou, Chang‐Pin; Chen, Junming; Chen, Wu-Nan

doi:10.1016/j.tsf.2007.03.028

Cited by 20 publications

(17 citation statements)

References 29 publications

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“…The water contact angle of PEG–silane modified glass surface was measured to be 34 ± 1°. These results are comparable with previously reported values for HMDS and PEG monolayer 22,29,30. In order to evaluate the effects of the PEG passivation procedure on HMDS monolayer, the glass substrate fully covered with HMDS monolayer was incubated in the PEG passivation solution for 24 h. The water contact angle was measured after the sample has been thoroughly rinsed and dried.…”

Section: Resultssupporting

confidence: 89%

Photolithographic patterning of organosilane monolayer for generating large area two-dimensional B lymphocyte arrays

2008

Lab Chip

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High-density live cell array serves as a valuable tool for the development of high-throughput immunophenotyping systems and cell-based biosensors. In this paper, we have, for the first time, demonstrated a simple fabrication process to form the hexamethyldisilazane (HMDS) and poly(ethylene glycol) (PEG) binary molecular surface which can be used to effectively form high fidelity cell arrays. The HMDS self-assembled monolayer (SAM) on glass substrates was photolithographically patterned and its ability to physically adsorb proteins was characterized by contact angle measurement and fluorescence microscopy respectively. Passivation of the non-HMDS coated background by PEG was verified to have no impact on the pre-patterned HMDS and greatly inhibited the non-specific protein binding. Using the biotin–streptavidin complexation as an intermediate, uniform orientation and high bioactivity were achieved for the immobilized B lymphocyte specific anti-CD19 antibodies and therefore ensured the formation of high resolution B lymphocyte arrays. The cell–ligand interaction specificity was investigated and the anti-CD19 decorated micropatterns presented a much higher cell-capturing rate (88%) than those modified by non-specific ligands (15% for anti-CD5 and 7% for streptavidin). The approach was verified to be biocompatible and the properties of the antibody-modified surface were maintained after 12 h cell culture. The HMDS monolayer formation and patterning processes, and the universal HMDS/biotin-BSA/streptavidin template, provide a very simple and convenient process to generate high resolution micropatterns of cell-adhesive ligands and are extendable to form arrays of other types of cells as well.

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Section: Resultssupporting

confidence: 89%

Photolithographic patterning of organosilane monolayer for generating large area two-dimensional B lymphocyte arrays

2008

Lab Chip

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“…The cause of this deterioration has often been attributed to the absorption of airborne contaminants and overall water vapour in the film pores, leading to an increase in the refractive index [8][9][10][11]. Additionally, it has been reported that, this adsorption degrades not only the optical properties but also the electrical properties of porous silica films [12,13]. On the other hand, some authors have found another deterioration process due to a slow diffusion process of Ca 2+ ions from the glass to the surface that leads to CaCO 3 formation on coating surface [14].…”

Section: Durability Of Antireflective Filmsmentioning

confidence: 99%

Long-term durability of sol–gel porous coatings for solar glass covers

et al. 2009

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“…17,18 Through condensation reaction, HMDS can react with the surface hydroxyl (eOH) groups to form a monolayer of siloxane (i.e., SieOeSi(CH 3 ) 3 ) through the following reaction:…”

Section: Resultsmentioning

confidence: 99%

Patterning Functional Proteins with High Selectivity for Biosensor Applications

Li¹,

2008

JALA: Journal of the Association for Laboratory Automation

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In this article, two-dimensional hexamethyldisilazane (HMDS) micropatterns were generated on glass substrates using photolithographic techniques for the assembly of functional proteins. The non-HMDS patterned areas were backfilled with poly(ethylene glycol) (PEG) silane to reduce the nonspecific protein adsorption. The hydrophobic methyl-terminated HMDS monolayer was verified to be favorable for physical protein adsorption with bovine serum albumin (BSA). The PEG-silane derivatized surface significantly reduced the BSA nonspecific binding by 97% compared to the pristine glass substrate so that high patterning selectivity was achieved. A universal streptavidin template was generated using preadsorbed biotinylated BSA on HMDS surface to sequentially bind additional biotinylated antibodies. Using this patterning strategy, the biotinylated goat anti-mouse (biotin-GAM) antibodies can be specifically recognized by the fluorescently labeled mouse immunoglobulin G, which indicated that the immobilized biotin-GAM was still bioactive. Also, the immobilized alkaline phosphatase was demonstrated to retain its enzymatic functionality by the ability to convert its fluorogenic substrate fluorescein diphosphate into fluorescent products. This simple and effective protein patterning technique can also be extended to create nanoscale protein arrays. Additionally, its adaptability for the assembly of arbitrary proteins and antibodies provides great potentials for biosensor and biomicroelectromechanical systems (MEMS) applications.

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The roles of hydrophobic group on the surface of ultra low dielectric constant porous silica film during thermal treatment

Cited by 20 publications

References 29 publications

Photolithographic patterning of organosilane monolayer for generating large area two-dimensional B lymphocyte arrays

Photolithographic patterning of organosilane monolayer for generating large area two-dimensional B lymphocyte arrays

Long-term durability of sol–gel porous coatings for solar glass covers

Patterning Functional Proteins with High Selectivity for Biosensor Applications

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