The roles of microRNA-34b-5p in angiogenesis of thyroid carcinoma

Maroof, Hamidreza; Islam, Farhadul; Ariana, Armin; Gopalan, Vinod

doi:10.1007/s12020-017-1393-3

Cited by 25 publications

(23 citation statements)

References 52 publications

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“…Additionally, genetic and epigenetic abnormalities in different molecules and growth factors have also been reported as risk factor in thyroid carcinogenesis. Among these growth factor, vascular endothelial growth factor (VEGF) is an important factor, encoded by the VEGF proto-oncogene (VEGF/VEGF-A), and is activated in hypoxic intracellular environments (Maroof et al, 2017). A subfamily of growth factors, VEGF-A acts as the main ligand that induces VEGFR-1 and VEGFR-2, the primary receptor involved in tumour angiogenesis (Holmes et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Expressional Analysis of Vascular Endothelial Growth Factor A (VEGFA) in Patients with Thyroid Cancer

Azhar¹

2018

Preprint

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Carcinogenesis of cells in the tissues of the thyroid gland leads to formation of thyroid cancer. Development of thyroid cancer involves a multifactorial route that is associated with various risk factors, such as exposure to ionizing radiation in early life, genetic predisposition, iodine intake in diet, environmental carcinogens, and genetic instability etc. These different risk factors can lead to a variety of mutations within proto-oncogenes and activating them as oncogenes. VEGF-A is one such proto-oncogene that plays a key role in tumour angiogenesis. The purpose of this study was to explore the association of respective gene with thyroid cancer pathogenesis. To test the hypothesis that VEGF-A expression patterns has influence in thyroid cancer metastasis and sustenance, a population based case-control study was conducted in 77 thyroid tumour samples along with adjacent control tissue samples. Quantitative real time-PCR (qPCR) was used for mRNA expression variation in VEGF-A gene. Expression analysis showed that VEGF-A expression level was very highly significantly downregulated (p<0.001) in tissues of thyroid cancer compared to adjacent control tissues. Non-significant (p>0.05) increase of VEGF-A expression mRNA with age group, gender, and advanced stages of T, N, and M stages was also observed in thyroid cancer samples compared to the controls. However, when compared for the expression levels relative to the proliferation marker, Ki67, very highly significant upregulation in thyroid tumour samples was seen in the same study cohort. Based on these results we can conclude that VEGF-A under expression at transcriptional level may be playing a key role in progression and/or spread of thyroid cancer.

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Section: Discussionmentioning

confidence: 99%

Expressional Analysis of Vascular Endothelial Growth Factor A (VEGFA) in Patients with Thyroid Cancer

Azhar¹

2018

Preprint

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“…4a). Interestingly, previous articles reported that miR-34b-5p regulates multiple cellular processes including cell apoptosis and cell proliferation through participating in a several critical signal pathway like VEGF-A, BCL2 and Notch [28]. To determine the interaction between BCL2 and miR-34b-5p, wild type 3'UTR (containing miR-34b-5p recognition site) and the mutant 3'UTR of BCL2 were cloned into luciferase reporter plasmids.…”

Section: Linc02418 Acts As a Cerna For Mir-34b-5p In Crc Cellsmentioning

confidence: 99%

LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis

Tian

Cui

et al. 2020

Preprint

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Background LncRNAs have been demonstrated to be functional regulators in tumor progression through interaction with various signaling pathways in multiple cancer types. However, the effect of LINC02418 on CRC progression still remains unclear. Methods LncRNA expression profile in CRC tissues was explored by using the TCGA database. The expressional level of LINC02418 in CRC patients was confirmed by qRT-PCR. Kaplan-Meier analyses was used to investigate the correlations between LINC02418 and OS of patients with CRC. After stably transducing sh-LINC02418 and sh-NC into HCT116 and LoVo cells, cell proliferative, migratory and invasive abilities were detected by CCK-8 assay, colony formation assay and trans-well assay, respectively. The binding between LINC02418 and miR-34b-5p, and the interaction between miR-34b-5p and BCL2 were determined by dual-luciferase assay. Western blot experiments were conducted to further explore the effect of miR-34b-5p on BCL2 signaling pathway. Rescue experiments were performed to uncover the role of LINC02418 /miR-34b-5p/ BCL2 axis in CRC progression. Results LINC02418 was upregulated in human colon cancer samples and its high expression correlated with poor prognosis. LINC02418 promoted cancer progression by enhancing tumor growth, cell mobility and invasiveness of colon cancer cells. Additionally, LINC02418 could physically bind to miR-34b-5p and subsequently interact with BCL2 signaling pathway. Down-regulation of LINC02418 reduced cell proliferation, but transfection of miR-34b-5p inhibitor or BCL2 in LINC02418-silenced colon cancer cells significantly promoted cell growth. Conclusions LINC02418 was upregulated in human colon cancer samples and could be used as indicator for prediction of prognosis. LINC02418 acted as a tumor driver by negatively regulating cell apoptosis through LINC02418 /miR-34b-5p/ BCL2 axis and in colorectal cancer.

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Section: Knockdown Of Linc02418 Inhibits Tumor Growth Cell Mobility mentioning

confidence: 99%

LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis

Tian

Cui

et al. 2020

Preprint

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Background LncRNAs act as functional regulators in tumor progression through interacting with various signaling pathways in multiple types of cancer. However, the effect of LINC02418 on colorectal cancer (CRC) progression and the underling mechanisms remain unclear.Methods LncRNA expression profile in CRC tissues was investigated by the TCGA database. The expressional level of LINC02418 in CRC patients was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Kaplan-Meier analyses was used to investigate the correlation between LINC02418 and overall survival (OS) of CRC patients. Cell proliferative, migratory and invasive abilities were detected by CCK-8 assays, colony formation assays and trans-well assays in HCT116 and LoVo cells which were stably transduced with sh-LINC02418 or sh-NC. The binding between LINC02418 and miR-34b-5p, and the interaction between miR-34b-5p and BCL2 were determined by dual-luciferase assays. Western blot experiments were conducted to further explore the effect of miR-34b-5p on BCL2 signaling pathway. Rescue experiments were performed to uncover the role of LINC02418 /miR-34b-5p/ BCL2 axis in CRC progression.Results LINC02418 was upregulated in human colon cancer samples when compared with adjacent tissue, and its high expressional level correlated with poor prognosis of CRC patients. LINC02418 promoted cancer progression by enhancing tumor growth, cell mobility and invasiveness of colon cancer cells. Additionally, LINC02418 could physically bind to miR-34b-5p and subsequently affect BCL2 signaling pathway. Down-regulation of LINC02418 reduced cell proliferation, while transfection of miR-34b-5p inhibitor or BCL2 into LINC02418-silenced CRC cells significantly promoted CRC cells growth.Conclusions LINC02418 was upregulated in human CRC samples and could be used as the indicator for prediction of prognosis. LINC02418 acted as a tumor driver by negatively regulating cell apoptosis through LINC02418 /miR-34b-5p/ BCL2 axis in CRC.

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The roles of microRNA-34b-5p in angiogenesis of thyroid carcinoma

Abstract: miR-34b-5p functions as a potent regulator of angiogenesis, apoptosis and cell proliferation via modulation of VEGF-A, Bcl-2 and Notch1 proteins. It could be a target for developing treatment strategies of thyroid carcinoma with aggressive clinical behaviour.

Cited by 25 publications

References 52 publications

Expressional Analysis of Vascular Endothelial Growth Factor A (VEGFA) in Patients with Thyroid Cancer

Expressional Analysis of Vascular Endothelial Growth Factor A (VEGFA) in Patients with Thyroid Cancer

LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis

LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis

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