The Ry-fsto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars

Abstract

A novel locus for extreme resistance to Potato virus Y (PVY), Ry-f sto , was identified on potato chromosome XII. The gene Ry-f sto has been introgressed from the wild potato species Solanum stoloniferum. Inheritance of Ry-f sto in the tetraploid potato population Rysto was consistent with the model of a single, dominant gene. Bulked segregant analysis identified an ISSR (inter-simple sequence repeat) marker UBC 857 980 linked to Ry-f sto . This marker mapped to linkage group XII of a reference potato RFLP (restriction fragment length polymorphism) map. Chromosome XII specific RFLP markers were converted into PCRbased STS and CAPS markers and tested for linkage with Ry-f sto in the population Rysto. CAPS marker GP122 718 was tightly linked to the resistance gene and was successfully used to identify Polish and German cultivars expressing extreme resistance to PVY. This indicates that the source of Ry-f sto has been widely utilized in various potato breeding programs and can be monitored by a diagnostic marker in markerassisted selection.

The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period

“…A pseudo-sequence characterized ampliWed region (pseudo-SCAR) marker was developed according to the procedure of Chagué et al (1996). PCR conditions for amplifying RAPD and pseudo-SCAR markers were according to Marczewski et al (1998), while ISSR markers were analyzed as described by Flis et al (2005). The Qiaquick kit (Qiagen, Hilden, Germany) was used to recover the ampliWed DNA from an agarose plug containing the marker OPB7+TG/GT.…”

Allele dosage of PVY resistance genes in potato clones using molecular markers

“…An explanation for the coincidence of amplification is that both markers are linked to the same gene, Ry (Flis et al 2005, Song et al 2005.…”

Review Article: Molecular Mechanisms of Resistance to Potato virus X and Y in Potato

“…One of them is originated from the wild species S. stoloniferum (gene Ry sto ) and mapped to chromosome XII (Flis et al, 2005;Song et al, 2005;Cernák et al, 2008). Although the Ry sto gene has not been identified yet, it is very probable that, as the majority of characterised viral resistance genes from plants, it belongs to the NBS-LRR class of resistance genes (Gururani et al, 2012).…”