The safety of β-carotene from Yarrowia lipolytica

Grenfell-Lee, Daniel; Zeller, Samuel; Cardoso, Renato; Pucaj, Kresimir

doi:10.1016/j.fct.2013.12.010

Cited by 32 publications

(16 citation statements)

References 18 publications

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“…The industrial use of a and has begun selling its products on the market. In addition, Grenfell- Lee et al (2014) showed safety tests on carotenoids that were produced by metabolic pathway…”

Section: Safety Studies On Products Of Yarrowia Lipolyticamentioning

confidence: 99%

Biotechnological applications of Yarrowia lipolytica: Past, present and future

Liu

Huang

2015

Biotechnology Advances

211

115

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Section: Safety Studies On Products Of Yarrowia Lipolyticamentioning

confidence: 99%

Biotechnological applications of Yarrowia lipolytica: Past, present and future

Liu

Huang

2015

Biotechnology Advances

211

115

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“…This is particularly true for Y. lipolytica for which the entire genome is sequenced and numerous genetic tools are available. Modified strains have been already developed to produce lipids with engineered composition matching the demands for biodiesel properties (Papanikolaou and Aggelis 2010) or novel compounds, for example, polyunsaturated fatty acids, and β-carotene (Chuang et al 2010;Grenfell-Lee et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Single cell oil production on molasses by Yarrowia lipolytica strains overexpressing DGA2 in multicopy

Gajdoš

Nicaud

Rossignol

et al. 2015

Appl Microbiol Biotechnol

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Yarrowia lipolytica is a promising platform for single cell oil production. It is well-known for its metabolism oriented toward utilization of hydrophobic substrates and accumulation of storage lipids. Multiple copies of DGA2 under constitutive promoter were introduced into the Q4 strain, a quadruple mutant deleted for the four acyltransferases (Δdga1, Δdga2, Δlro1, and Δare1) to improve lipid accumulation. The Q4-DGA2 x3 strain contains three copies of DGA2. Further increase in accumulation was accomplished by blocking the β-oxidation pathway through MFE1 gene deletion yielding Q4-Δmfe DGA2 x3. In order to use molasses as a substrate for single cell oil production, sucrose utilization was established by expressing the Saccharomyces cerevisiae SUC2 gene yielding Q4-SUC2 DGA2 x3 and Q4-Δmfe SUC2 DGA2 x3. During cultivation on sucrose medium with a carbon to nitrogen ratio of 80, both strains accumulated more than 40 % of lipids, which was a 2-fold increase in lipid storage. Q4-Δmfe SUC2 DGA2 x3 accumulated more lipids than Q4-SUC2 DGA2 x3 (49 vs. 43 %) but yielded less biomass (13.7 vs. 15 g/L). When grown on 8 % (v/v) molasses, both strains accumulated more than 30 % of lipids after 3 days, while biomass yield was higher in Q4-SUC2 DGA2 x3 (16.4 vs. 14.4 g/L). Further addition of molasses at 72 h resulted in higher biomass yield, 26.6 g/L for Q4-SUC2 DGA2 x3, without modification of lipid content. This work presents genetically modified strains of Y. lipolytica as suitable tools for direct conversion of industrial molasses into value added products based on single cell oils.

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“…Another metabolic engineering approach produced 16 mg/gDCW of lycopene, the direct precursor of β‐carotene (Matthaus, Ketelhot, Gatter, & Barth, ). Importantly, Grenfell‐Lee, Zeller, Cardoso, and Pucaj () have demonstrated that the safety profile of the β‐carotene produced in Y. lipolytica is the same as other commercial products, which would facilitate its commercialization. Therefore, this oleaginous yeast represents a promising biotechnological chassis for the production of β‐carotene.…”

Section: Introductionmentioning

confidence: 99%

A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β‐carotene

Larroude

Celińska

Back

et al. 2017

Biotech & Bioengineering

275

237

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The increasing market demands of β-carotene as colorant, antioxidant and vitamin precursor, requires novel biotechnological production platforms. Yarrowia lipolytica, is an industrial organism unable to naturally synthesize carotenoids but with the ability to produce high amounts of the precursor Acetyl-CoA. We first found that a lipid overproducer strain was capable of producing more β-carotene than a wild type after expressing the heterologous pathway. Thereafter, we developed a combinatorial synthetic biology approach base on Golden Gate DNA assembly to screen the optimum promoter-gene pairs for each transcriptional unit expressed. The best strain reached a production titer of 1.5 g/L and a maximum yield of 0.048 g/g of glucose in flask. β-carotene production was further increased in controlled conditions using a fed-batch fermentation. A total production of β-carotene of 6.5 g/L and 90 mg/g DCW with a concomitant production of 42.6 g/L of lipids was achieved. Such high titers suggest that engineered Y. lipolytica is a competitive producer organism of β-carotene.

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The safety of β-carotene from Yarrowia lipolytica

Cited by 32 publications

References 18 publications

Biotechnological applications of Yarrowia lipolytica: Past, present and future

Biotechnological applications of Yarrowia lipolytica: Past, present and future

Single cell oil production on molasses by Yarrowia lipolytica strains overexpressing DGA2 in multicopy

A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β‐carotene

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