The salivary mucin MUC5B and lactoperoxidase can be used for layer-by-layer film formation

Lindh, Liselott; Svendsen, Ida; Svensson, Olof; Cárdenas, Marité; Arnebrant, Thomas

doi:10.1016/j.jcis.2007.01.086

Cited by 36 publications

(25 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the specific and reversible adsorption of human serum albumin to a synthetically modified dextran matrix [34]. Although the in vivo performance of that particular coating has been disputed [35], the present work together with a recent study performed on combined layers of human MUC5B mucin and lactoperoxidase [36], suggest that further attention should be given the use of mucins as biomaterial surface matrices for support of biologically relevant components. Particularly, future research will focus on the interactions of neutrophils with composite coatings of mucin and specific human host proteins commonly encountered during implantation.…”

Section: Discussionmentioning

confidence: 81%

Interactions between human neutrophils and mucin-coated surfaces

Sandberg

Carlsson

Ott

2008

J Mater Sci: Mater Med

View full text Add to dashboard Cite

Recently, we showed microscopically that bovine (BSM), porcine (PGM) and human (MG1) mucin coatings could suppress the adhesion of neutrophils to a polyethylene terephthalate-based model biomaterial (Thermanox). Here, using the release of reactive oxygen species (ROS) as a marker of material-induced neutrophil activation, the strong surface-passivating effects of these mucin coatings were corroborated. Under optimal adsorption conditions, all mucin species performed equally well, thus indicating a high degree of functional homology between the mucins. Cell adhesion and morphology correlated well with the release of ROS. Quartz crystal microbalance (QCM-D) analysis linked low neutrophil activation to efficient mucin surface-shielding. Interestingly, the shielding power appeared equal for thick expanded and thin compact mucin coatings. Combined mucin-serum coatings were found to be highly surface-passivating. Particularly, since our data suggested partly synergistic mucin-serum action, we highlight the possibility that pre-adsorbed mucins could provide favorable support for adsorbing host components.

show abstract

Section: Discussionmentioning

confidence: 81%

Interactions between human neutrophils and mucin-coated surfaces

Sandberg

Carlsson

Ott

2008

J Mater Sci: Mater Med

View full text Add to dashboard Cite

show abstract

“…Wickstrom et al [28] demonstrated macromolecular organization of saliva and identification of "insoluble" MUC5B assemblies and non-mucin proteins in the gel phase. It has earlier been reported that MUC5B and lactoperoxidase can be used to build multilayers on hydrophobic and hydrophilic silica surfaces [9]. In our studies human salivary mucin (MUC5B) adsorption on a gold surface has been investigated (Fig.…”

Section: Lpo Adsorption On Au-muc5b Surfacementioning

confidence: 93%

“…Second, the amounts were based on assumption of the same dn/dc for all proteins. For example, the dn/dc for mucin has been reported to 0.16 mL/g [9], resulting in a possible underestimation of the amount of mucin of about 10% but provided that no exchange takes place this does not affect the activity data. In the case of MUC5B adsorption at the low concentration (0.5 g/mL), however, the small amount of adsorbed (exchangeable) MUC5B will make the possible error small.…”

Section: Activity Measurements Of Immobilised Lactoperoxidasementioning

confidence: 99%

“…The majority of the applications rely on the addition of LPO to a homogeneous product phase or immobilisation of LPO on the surface, e.g., of a packaging material [7] or dental (titanium) implants [8]. The development of new methods on how LPO could be introduced into different products is continuously evaluated; one of the recently demonstrated examples describes a possibility of a multilayer assembly of LPO in combination with other proteins using layer-by-layer deposition [9]. In this and other applications, where LPO is adsorbed on a surface, the measurements of the enzymatic activity would be of the high interest.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Activity of lactoperoxidase when adsorbed on protein layers

Haberska

Svensson

Shleev

et al. 2008

Talanta

Self Cite

View full text Add to dashboard Cite

This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. Contents lists available at ScienceDirect Talanta j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / t a l a n t a b s t r a c tLactoperoxidase (LPO) is an enzyme, which is used as an antimicrobial agent in a number of applications, e.g., food technology. In the majority of applications LPO is added to a homogeneous product phase or immobilised on product surface. In the latter case, however, the measurements of LPO activity are seldom reported. In this paper we have assessed LPO enzymatic activity on bare and protein modified gold surfaces by means of electrochemistry. It was found that LPO rapidly adsorbs to bare gold surfaces resulting in an amount of LPO adsorbed of 2.9 mg/m 2 . A lower amount of adsorbed LPO is obtained if the gold surface is exposed to bovine serum albumin, bovine or human mucin prior to LPO adsorption. The enzymatic activity of the adsorbed enzyme is in general preserved at the experimental conditions and varies only moderately when comparing bare gold and gold surface pretreated with the selected proteins. The measurement of LPO specific activity, however, indicate that it is about 1.5 times higher if LPO is adsorbed on gold surfaces containing a small amount of preadsorbed mucin in comparison to the LPO directly adsorbed on bare gold.

show abstract

“…MAM plays a very important role in protection and lubrication of oral mucosa [3]. The MAM attaches to the ''microplicae'' with the help of membrane anchored ''mucous binding proteins'' [4]. This MAM then acts as a scaffold for the formation of highly hydrated and viscous gel called salivary mucous gel (SMG) [5].…”

Section: 1mentioning

confidence: 99%

Surface Morphology of Superficial Cells in Irradiated Oral Mucosa

Sarode

2014

Ultrastructural Pathology

View full text Add to dashboard Cite

The salivary mucin MUC5B and lactoperoxidase can be used for layer-by-layer film formation

Cited by 36 publications

References 45 publications

Interactions between human neutrophils and mucin-coated surfaces

Interactions between human neutrophils and mucin-coated surfaces

Activity of lactoperoxidase when adsorbed on protein layers

Surface Morphology of Superficial Cells in Irradiated Oral Mucosa

Contact Info

Product

Resources

About