The SARS-CoV-2 receptor ACE2 is expressed in mouse pericytes but not endothelial cells: Implications for COVID-19 vascular research

Muhl, Lars; He, Liqun; Sun, Ying; Mäe, Maarja Andaloussi; Pietilä, Riikka; Liu, Jianping; Genové, Guillem; Zhang, Lei; Xie, Yuan; Leptidis, Stefanos; Mocci, Giuseppe; Stritt, Simon; Osman, Ahmed; Anisimov, Andrey; Hemanthakumar, Karthik Amudhala; Räsänen, Markus; Hansson, Emil M.; Björkegren, Johan; Vanlandewijck, Michael; Blomgren, Klas; Mäkinen, Taija; Peng, Xiaorong; Hu, Yizhou; Ernfors, Patrik; Arnold, Thomas D.; Alitalo, Kari; Lendahl, Urban; Betsholtz, Christer

doi:10.1016/j.stemcr.2022.03.016

Cited by 57 publications

(44 citation statements)

References 55 publications

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“…We, therefore, concluded that the ACE2, observed in a small proportion of the endothelial cells, must have been contributed by contaminating fragments of pericytes attached to the endothelial cells. These conclusions were confirmed in a subsequent study in the mouse, in which we found that Ace2 (mouse ACE2) is not expressed by endothelial cells in any organ, but instead organotypically by pericytes in heart and brain, leading to contamination of a small proportion of the endothelial cells in these organs (68). Reciprocal contamination by neighboring cells must, therefore, always be considered as a possible confounder in scRNASeq data.…”

Section: Contamination In Scrnaseq Datasupporting

confidence: 77%

Toward a granular molecular-anatomic map of the blood vasculature – single-cell RNA sequencing makes the leap

Betsholtz

2022

ujms

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Single-cell RNA sequencing (scRNAseq) marks the birth of a new era in physiology and medicine. Within foreseeable future, we will know exactly what genes are expressed – and at what levels – in all the different cell types and subtypes that make up our bodies. We will also learn how a particular cell state, whether it occurs during development, tissue repair, or disease, reflects precise changes in gene expression. While profoundly impacting all areas of life science, scRNAseq may lead to a particular leap in vascular biology research. Blood vessels pervade and fulfill essential functions in all organs, but the functions differ. Innumerable organ-specific vascular adaptations and specializations are required. These, in turn, are dictated by differential gene expression by the two principal cellular building blocks of blood vessels: endothelial cells and mural cells. An organotypic vasculature is essential for functions as diverse as thinking, gas exchange, urine excretion, and xenobiotic detoxification in the brain, lung, kidney, and liver, respectively. In addition to the organotypicity, vascular cells also differ along the vascular arterio-venous axis, referred to as zonation, differences that are essential for the regulation of blood pressure and flow. Moreover, gene expression-based molecular changes dictate states of cellular activity, necessary for angiogenesis, vascular permeability, and immune cell trafficking, i.e. functions necessary for development, inflammation, and repair. These different levels of cellular heterogeneity create a nearly infinite phenotypic diversity among vascular cells. In this review, I summarize and exemplify what scRNAseq has brought to the picture in just a few years and point out where it will take us.

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Section: Contamination In Scrnaseq Datasupporting

confidence: 77%

Toward a granular molecular-anatomic map of the blood vasculature – single-cell RNA sequencing makes the leap

Betsholtz

2022

ujms

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“…Consistently, perivascular cells (Pc) that embrace capillary ECs, regulating vascular permeability and stabilizing vessels, express the highest levels of ACE2, proved by in vivo studies [21] , [22] , [23] . Nonetheless, although in vitro data did not support the infectivity of cardiac Pc either [24] , the presence of viral dsRNA was shown in brain Pc of patients that died of COVID-19 [25] .…”

Section: Molecular Mechanisms Of Sars-cov-2-induced Cardiac Microvasc...mentioning

confidence: 89%

Long-term effect of SARS-CoV-2 infection on cardiovascular outcomes and all-cause mortality

et al. 2022

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“…Therefore, SARS-CoV-2 infects cells by binding its S protein to cell surface receptors. The latter is followed by S protein cleavage by TMPRSS2 or cathepsins L or B, the fusion of viral and cellular membranes, and viral RNA entry into the cytoplasm [70] .…”

Section: Sars-cov-2 and Infection Mechanismsmentioning

confidence: 99%

Functional consequences of SARS-CoV-2 infection in pregnant women, fetoplacental unit, and neonate

Carvajal

Casanello

Farías

et al. 2023

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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The SARS-CoV-2 receptor ACE2 is expressed in mouse pericytes but not endothelial cells: Implications for COVID-19 vascular research

Cited by 57 publications

References 55 publications

Toward a granular molecular-anatomic map of the blood vasculature – single-cell RNA sequencing makes the leap

Toward a granular molecular-anatomic map of the blood vasculature – single-cell RNA sequencing makes the leap

Long-term effect of SARS-CoV-2 infection on cardiovascular outcomes and all-cause mortality

Functional consequences of SARS-CoV-2 infection in pregnant women, fetoplacental unit, and neonate

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