The scanned nanopipette: a new tool for high resolution bioimaging and controlled deposition of biomolecules

Ying, Liming; Bruckbauer, Andreas; Zhou, Dejian; Gorelik, Julia; Shevchuk, Andrew; Lab, Max J.; Korchev, Yuri E.; Klenerman, David

doi:10.1039/b506743j

Cited by 107 publications

(88 citation statements)

References 56 publications

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“…SICM has already achieved this condition (16,35). With the use of very sharp glass capillaries with a small pore at the apex, the spatial resolution of SICM has reached a few nanometers (117). Immobile protein molecules with a size of ∼14 nm in live cell membranes have been successfully imaged (84).…”

Section: High-speed Noncontact Imagingmentioning

confidence: 89%

High-Speed AFM and Applications to Biomolecular Systems

Ando

Uchihashi

Kodera

2013

Annu. Rev. Biophys.

255

216

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Directly observing individual protein molecules in action at high spatiotemporal resolution has long been a holy grail for biological science. This is because we long have had to infer how proteins function from the static snapshots of their structures and dynamic behavior of optical makers attached to the molecules. This limitation has recently been removed to a large extent by the materialization of high-speed atomic force microscopy (HS-AFM). HS-AFM allows us to directly visualize the structure dynamics and dynamic processes of biological molecules in physiological solutions, at subsecond to sub-100-ms temporal resolution, without disturbing their function. In fact, dynamically acting molecules such as myosin V walking on an actin filament and bacteriorhodopsin in response to light are successfully visualized. In this review, we first describe theoretical considerations for the highest possible imaging rate of this new microscope, and then highlight recent imaging studies. Finally, the current limitation and future challenges to explore are described.

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Section: High-speed Noncontact Imagingmentioning

confidence: 89%

High-Speed AFM and Applications to Biomolecular Systems

Ando

Uchihashi

Kodera

2013

Annu. Rev. Biophys.

255

216

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“…Owing to the progress in fabrication techniques for producing very sharp glass capillaries with a small pore at the apex, the spatial resolution of ICSPM has reached a few nm [195]. Immobile protein molecules with a size of ~14 nm on living cell membranes have been successfully imaged [196].…”

Section: Non-contact High-speed Afmmentioning

confidence: 99%

High-speed atomic force microscopy coming of age

Ando¹

2012

Nanotechnology

325

246

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High-speed atomic force microscopy (HS-AFM) is now materialized. It allows direct visualization of dynamic structural changes and dynamic processes of functioning biological molecules in physiological solutions, at high spatiotemporal resolution. Dynamic molecular events unselectively appear in detail in an AFM movie, facilitating our understanding of how biological molecules operate to function. This review describes a historical overview of technical development towards HS-AFM, summarizes elementary devices and techniques used in the current HS-AFM, and then highlights recent imaging studies. Finally, future challenges of HS-AFM studies are briefly discussed.

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“…Owing to the progress in fabrication techniques for producing very sharp glass capillaries with a small pore at the apex, the spatial resolution of ICSPM has reached a few nm [221]. Immobile protein molecules with a size of ~14 nm on living cell membranes have been successfully imaged [222].…”

Section: Imaging Speed Interaction Force and Noncontact Imagingmentioning

confidence: 99%

High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes

Ando

Uchihashi

Fukuma

2008

Progress in Surface Science

496

414

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The atomic force microscope (AFM) has a unique capability of allowing the high-resolution imaging of biological samples on substratum surfaces in physiological solutions. Recent technological progress of AFM in biological research has resulted in remarkable improvements in both the imaging rate and the tip force acting on the sample. These improvements have enabled the direct visualization of dynamic structural changes and dynamic interactions occurring in individual biological macromolecules, which is currently not possible with other techniques. Therefore, high-speed AFM is expected to have a revolutionary impact on biological sciences. In addition, the recently achieved atomic resolution in liquids will further expand the usefulness of AFM in biological research. In this article, we first describe the various capabilities required of AFM in biological sciences, which is followed by a detailed description of various devices and techniques developed for high-speed AFM and atomic-resolution in-liquid AFM. We then describe various imaging studies performed using our cutting-edge microscopes and their current capabilities as well as their limitations, and conclude by discussing the future prospects of AFM as an imaging tool in biological research.

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The scanned nanopipette: a new tool for high resolution bioimaging and controlled deposition of biomolecules

Cited by 107 publications

References 56 publications

High-Speed AFM and Applications to Biomolecular Systems

High-Speed AFM and Applications to Biomolecular Systems

High-speed atomic force microscopy coming of age

High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes

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