The scent of Mycobacterium tuberculosis – Part II breath

Syhre, Mona; Manning, Laurens; Phuanukoonnon, Suparat; Harino, Paul; Chambers, Stephen T.

doi:10.1016/j.tube.2009.04.003

Cited by 134 publications

(89 citation statements)

References 6 publications

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“…In fact, a comparison of the analysis of breath samples from nonsmoking patients with TB and those without TB showed a striking difference in levels of nicotinic acid between the two groups (69). In a subsequent study, this same group reported the detection of methylnicotinate, a metabolite of nicotinic acid, in the exhaled breath of TB patients, achieving a sensitivity of 84% but a low specificity of 64% among patients with smear-positive TB (70).…”

Section: Respiratory Infectionsmentioning

confidence: 99%

Clinical Application of Volatile Organic Compound Analysis for Detecting Infectious Diseases

2013

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SUMMARY This review article introduces the significance of testing of volatile organic compounds (VOCs) in clinical samples and summarizes important features of some of the technologies. Compared to other human diseases such as cancer, studies on VOC analysis in cases of infectious diseases are limited. Here, we have described results of studies which have used some of the appropriate technologies to evaluate VOC biomarkers and biomarker profiles associated with infections. The publications reviewed include important infections of the respiratory tract, gastrointestinal tract, urinary tract, and nasal cavity. The results highlight the use of VOC biomarker profiles resulting from certain infectious diseases in discriminating between infected and healthy subjects. Infection-related VOC profiles measured in exhaled breath as well as from headspaces of feces or urine samples are a source of information with respect to disease detection. The volatiles emitted in clinical matrices may on the one hand represent metabolites of the infecting pathogen or on the other hand reflect pathogen-induced host responses or, indeed, a combination of both. Because exhaled-breath samples are easy to collect and online instruments are commercially available, VOC analysis in exhaled breath appears to be a promising tool for noninvasive detection and monitoring of infectious diseases.

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Section: Respiratory Infectionsmentioning

confidence: 99%

Clinical Application of Volatile Organic Compound Analysis for Detecting Infectious Diseases

2013

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“…However, most of the microbial VOCs are released in very low concentrations requiring and the exact composition of the emitted volatile compounds varies with different environmental conditions. Nevertheless, detection of microbial VOCs has great potential to be used in pathogen-directed breath testing, as currently seen for Mycobacterium tuberculosis [7,8] or the CF pathogens…”

Section: Introductionmentioning

confidence: 99%

A rapid method for breath analysis in cystic fibrosis patients

Kramer

Sauer-Heilborn

Welte

et al. 2014

Eur J Clin Microbiol Infect Dis

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Purpose: For easy handling and speed of lung diseases diagnostic, approaches based on volatile organic compounds (VOCs), including those emitted by pathogenic microorganisms, are considered but currently require considerable sampling efforts. We tested whether easy to handle and fast detection of lung infections is possible using Solid-Phase-Micro-Extraction (SPME) of 100 ml of exhaled breath. Methods: An analytical procedure for the detection of VOCs from the headspace of epithelial lung cells infected with four human pathogens was developed. The feasibility of this method was tested in a cystic fibrosis (CF) outpatient clinic in vivo. Exhaled breath was extracted by SPME and analyzed by gas chromatography-mass spectrometry. Results: The compositions of VOCs released in the infection model were characteristic for all individual pathogens tested.Exhaled breath of CF patients allowed clear distinction of CF patients and controls by their VOC compositions using multivariate analyses. Interestingly, the major specific VOCs detected in exhaled breath of infected CF patients in vivo differed from those monitored during bacterial in vitro growth. Conclusions: SPME extraction of VOCs from 100 ml of human breath allowed the distinction between CF patients and healthy probands. Our results highlight the importance of assessing the entire pattern of VOCs instead of selected biomarkers for diagnostic purposes, as well as the need for using clinical samples to identify reliable biomarkers. This study provides the proof-of-concept for the approach using the composition of exhaled VOCs in human breath for rapid identification of infectious agents in patients with lower respiratory tract infections.

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“…Interestingly, the inferred accumulation of NaMN in non-replicating Mtb may provide a rationale for the well known presence of nicotinic acid in the breath of tuberculosis patients (41). Indeed, in our recent study we observed an appreciable excretion of nicotinic acid after accumulation and degradation of NaMN (e.g.…”

Section: Discussionmentioning

confidence: 58%

Mycobacterial Nicotinate Mononucleotide Adenylyltransferase

Rodionova

Zuccola

Sorci

et al. 2015

Journal of Biological Chemistry

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Background: NAD biosynthesis was implicated as an antibacterial target pathway. Results: Structure-functional analysis of Mycobacterium tuberculosis NadD revealed an over-closed conformation and a sequential kinetic mechanism. Conclusion: M. tuberculosis NadD is a potentially druggable target suitable for the development of selective inhibitors. Significance: This study describes a novel regulatory mechanism and points to a specific strategy for targeting mycobacterial NadD.

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The scent of Mycobacterium tuberculosis – Part II breath

Cited by 134 publications

References 6 publications

Clinical Application of Volatile Organic Compound Analysis for Detecting Infectious Diseases

Clinical Application of Volatile Organic Compound Analysis for Detecting Infectious Diseases

A rapid method for breath analysis in cystic fibrosis patients

Mycobacterial Nicotinate Mononucleotide Adenylyltransferase

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