Strategic transformation of regionalization for agricultural comprehensive development (ACD) was presented by the Ministry of Finance of the People's Republic of China (MOF) in 2014. The regionalization is the premise and basis of the sustainable development and improved competitiveness for agriculture. Based on the environmental resources related to agriculture, such as cropland, climate, water resources, terrain, geomorphology, patterns of the ACD projects, distribution of ecological planning, etc., we devised 13 indices using the geographic comprehensive regionalization method. The indices took into account a combination of dynamic and static, qualitative and quantitative, as well as agricultural and ecological factors. The strategic transformation of regionalization for the ACD in Ningxia Hui Autonomous Region of China was performed; seven types were included: prioritized regions, prioritized and restricted regions, protected regions, protected and restricted regions, restricted and prioritized regions, restricted and protected regions, and restricted regions. A further 24 subtypes were used based on locations and ecological zones. The regionalization results showed that prioritized regions were mainly in northern Ningxia, the most suitable area for agriculture. The protected and restricted regions were in central and southern Ningxia. In the central part, drought was the limiting factor for agriculture, and water conservation projects there should be supported. The ecological environment is fragile in southern Ningxia, so there is a need for ecologically sound agriculture to be developed in this region. Such regionaliza-1676 Journal of Geographical Sciences tion could achieve two goals, namely agricultural conservation and eco-environmental protection. It was performed following the requirement for scientific regionalization to include three types of regions (prioritized regions, protected regions, and restricted regions), and was applied at the township scale in a provincial or autonomous region for the first time. The results provide both guidance for the strategic transformation of the ACD in Ningxia, and a reference for similar work in other provinces.