The <scp><i>Saccharomyces cerevisiae</i></scp> poly (A) binding protein (Pab1): Master regulator of mRNA metabolism and cell physiology

Brambilla, Marco; Martani, Francesca; Bertacchi, Stefano; Vitangeli, Ilaria; Branduardi, Paola

doi:10.1002/yea.3347

Cited by 28 publications

(42 citation statements)

References 97 publications

(204 reference statements)

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“…Five strains were identified as M. guilliermondii and one could not be differentiated between M. guilliermondii and M. caribbica . It was shown that these two strains have an almost identical fermentation profile and are identified by its characteristic of producing coenzyme-Q9, and the separation between them is uncertain [25,26]. The species of the genus Meyerozyma belong to the phylum Ascomycota and are inserted in the clade Saccharomycotina CTG.…”

Section: Discussionmentioning

confidence: 99%

“…The species of the genus Meyerozyma belong to the phylum Ascomycota and are inserted in the clade Saccharomycotina CTG. However, the phylogenetic structure of the yeasts of this group is not fully understood [24,25,26,27]. Even if the scientific advances enabled the proper identification of some yeasts from this clade, it was pointed out that one of the major problems in this identification is the range of incorrect or non-updated taxonomic annotations in public databases addressing Meyerozyma species [28,29].…”

Section: Discussionmentioning

confidence: 99%

“…Although, the screened microbial collection has a variety of yeast genera, such as Candida , Spathaspora , Pichia , and Wickerhamomyces [7], and all of the selected yeasts in this work were identified as the genus Meyerozyma . This fact can be explained by the ubiquity of the species of this genus, they can be isolated from different environments [30] (thermal waters, fruits, insects, and soil), as well as standing out for its ability to grow fast on pentoses, such as xylose [24,25,26]. Indeed, a previous screening based on the capability to tolerate sugarcane bagasse hydrolysate with a high content of acetic acid (~7 g/L), employing some of the same strains of this study, resulted in the selection of Candida tropicalis strains [7].…”

Section: Discussionmentioning

confidence: 99%

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Xylitol Production: Identification and Comparison of New Producing Yeasts

2019

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Xylitol is a sugar alcohol with five carbons that can be used in the pharmaceutical and food industries. It is industrially produced by chemical route; however, a more economical and environmentally friendly production process is of interest. In this context, this study aimed to select wild yeasts able to produce xylitol and compare their performance in sugarcane bagasse hydrolysate. For this, 960 yeast strains, isolated from soil, wood, and insects have been prospected and selected for the ability to grow on defined medium containing xylose as the sole carbon source. A total of 42 yeasts was selected and their profile of sugar consumption and metabolite production were analyzed in microscale fermentation. The six best xylose-consuming strains were molecularly identified as Meyerozyma spp. The fermentative kinetics comparisons on defined medium and on sugarcane bagasse hydrolysate showed physiological differences among these strains. Production yields vary from YP/S = 0.25 g/g to YP/S = 0.34 g/g in defined medium and from YP/S = 0.41 g/g to YP/S = 0.60 g/g in the hydrolysate. Then, the xylitol production performance of the best xylose-consuming strain obtained in the screening, which was named M. guilliermondii B12, was compared with the previously reported xylitol producing yeasts M. guilliermondii A3, Spathaspora sp. JA1, and Wickerhamomyces anomalus 740 in sugarcane bagasse hydrolysate under oxygen-limited conditions. All the yeasts were able to metabolize xylose, but W. anomalus 740 showed the highest xylitol production yield, reaching a maximum of 0.83 g xylitol/g of xylose in hydrolysate. The screening strategy allowed identification of a new M. guilliermondii strain that efficiently grows in xylose even in hydrolysate with a high content of acetic acid (~6 g/L). In addition, this study reports, for the first time, a high-efficient xylitol producing strain of W. anomalus, which achieved, to the best of our knowledge, one of the highest xylitol production yields in hydrolysate reported in the literature.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Xylitol Production: Identification and Comparison of New Producing Yeasts

2019

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“…Cytoplasmic membraneless condensates concentrate biomolecules locally in response to signal transduction to accelerate biochemical reactions (Hernández-Vega et al, 2017;Woodruff et al, 2017) or to adapt to environmental stress (Buchan et al, 2008;Kroschwald et al, 2018;Riback et al, 2017;Xie et al, 2019). When cells are stressed and enter a quiescent state, immiscible condensates store the functional macromolecules; once the stress is released, these molecules are dissolved into the dilute phase and can be utilized for various cellular functions (Franzmann et al, 2018;Marco et al, 2018;Xie et al, 2019). If these biomolecular condensates turn into gel or solid states, this will lead to a delay in or irreversible dissolving of functional macromolecules, which can be detrimental for the cell, resulting in dysfunctional or disease states, such as the pathological fibrillization of RNA-binding proteins, such as superoxide dismutase 1 (SOD1) (Grabocka and Bar-Sagi, 2016;Mateju et al, 2017;Molliex et al, 2015).…”

Section: Polarisome Components and Their Interactionsmentioning

confidence: 99%

Polarisome assembly mediates actin remodeling during polarized yeast and fungal growth

Xie

Miao

2021

Journal of Cell Science

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Dynamic assembly and remodeling of actin is critical for many cellular processes during development and stress adaptation. In filamentous fungi and budding yeast, actin cables align in a polarized manner along the mother-to-daughter cell axis, and are essential for the establishment and maintenance of polarity; moreover, they rapidly remodel in response to environmental cues to achieve an optimal system response. A formin at the tip region within a macromolecular complex, called the polarisome, is responsible for driving actin cable polymerization during polarity establishment. This polarisome undergoes dynamic assembly through spatial and temporally regulated interactions between its components. Understanding this process is important to comprehend the tuneable activities of the formin-centered nucleation core, which are regulated through divergent molecular interactions and assembly modes within the polarisome. In this Review, we focus on how intrinsically disordered regions (IDRs) orchestrate the condensation of the polarisome components and the dynamic assembly of the complex. In addition, we address how these components are dynamically distributed in and out of the assembly zone, thereby regulating polarized growth. We also discuss the potential mechanical feedback mechanisms by which the force-induced actin polymerization at the tip of the budding yeast regulates the assembly and function of the polarisome.

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“…RNA-binding proteins (RBPs) contain designated domains to interact with specific elements in target RNAs. For example, the RNA recognition motifs (RRMs) of the poly(A)-binding protein recognises the poly(A) tail of almost all mRNAs (Brambilla et al, 2019;Hogan et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Inside-Out: From Endosomes to Extracellular Vesicles in Fungal RNA Transport

Kwon

Tisserant

Tulinski

et al. 2019

Preprint

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Membrane-coupled RNA transport is an emerging theme in fungal biology. This review focuses on the RNA cargo and mechanistic details of transport via two inter-related sets of organelles: endosomes and extracellular vesicles for intra- and intercellular RNA transfer. Simultaneous transport and translation of messenger RNAs (mRNAs) on the surface of shuttling endosomes is a conserved process pertinent to highly polarised eukaryotic cells, such as hyphae or neurons. Here we detail the endosomal mRNA transport machinery components and mRNA targets of the core RNA-binding protein Rrm4. Extracellular vesicles (EVs) are newly garnering interest as mediators of intercellular communication, especially between pathogenic fungi and their hosts. Landmark studies in plant-fungus interactions indicate EVs as a means of delivering various cargos, most notably small RNAs (sRNAs), for cross-kingdom RNA interference. Recent advances and implications of the nascent field of fungal EVs are discussed and potential links between endosomal and EV-mediated RNA transport are proposed.

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The Saccharomyces cerevisiae poly (A) binding protein (Pab1): Master regulator of mRNA metabolism and cell physiology

Cited by 28 publications

References 97 publications

Xylitol Production: Identification and Comparison of New Producing Yeasts

Xylitol Production: Identification and Comparison of New Producing Yeasts

Polarisome assembly mediates actin remodeling during polarized yeast and fungal growth

Inside-Out: From Endosomes to Extracellular Vesicles in Fungal RNA Transport

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