The second extracellular domain of connexin 50 is important for in cell adhesion, lens differentiation, and adhesion molecule expression

Li, Zhen; Quan, Yumeng; Wang, Guangyan; Ma, Bo; Gu, Sumin; Jiang, Jean X.

doi:10.1016/j.jbc.2023.102965

Cited by 4 publications

(3 citation statements)

References 48 publications

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“…Additionally, fibers contain the spectrin-actin cytoskeleton tethered to the membrane (Cheng et al, 2017). Fiber cell shape, adhesion, compaction and microcirculation rely on the organization and activities of various membrane proteins, including cell adhesion, transport, channel, and signaling proteins (Cheng et al, 2017, Mathias et al, 2010, Li et al, 2022, Maddala et al, 2013, Gu et al, 2019, Shiels and Bassnett, 1996, Wang et al, 2016, Li et al, 2023). Scaffolding proteins linking the spectrin-actin cytoskeleton to membrane proteins are essential for their stability and activity (Bennett and Lorenzo, 2016).…”

Section: Discussionmentioning

confidence: 99%

Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics, and clarity

Maddala,

Allen,

Skiba

et al. 2024

Preprint

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Summary StatementThis study illustrates a vital role for ankyrin-B in lens architecture, growth and function through its involvement in membrane protein and spectrin-actin cytoskeletal organization and stabilityThe transparent ocular lens is essential for vision by focusing light onto the retina. Despite recognizing the importance of its unique cellular architecture and mechanical properties, the molecular mechanisms governing these attributes remain elusive. This study aims to elucidate the role of ankyrin-B (AnkB), a membrane scaffolding protein, in lens cytoarchitecture, growth and function using a conditional knockout (cKO) mouse model. AnkB cKO mouse has no defects in lens morphogenesis, but exhibited changes that supported a global role for AnkB in maintenance of lens clarity, size, cytoarchitecture, and stiffness. Notably, absence of AnkB led to nuclear cataract formation, evident from P16. AnkB cKO lens fibers exhibit progressive disruption in membrane organization of the spectrin-actin cytoskeleton, channel proteins, cell-cell adhesion, shape change, loss and degradation of several membrane proteins (e.g., NrCAM. N-cadherin and aquaporin-0) along with a disorganized plasma membrane and impaired ball-and-socket membrane interdigitations. Furthermore, absence of AnkB led to decreased lens stiffness. Collectively, these results illustrate the essential role for AnkB in lens architecture, growth and function through its involvement in membrane protein and cytoskeletal organization.

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Section: Discussionmentioning

confidence: 99%

Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics, and clarity

Maddala,

Allen,

Skiba

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…For example, the Cx43 intracellular interactome shares members with the interactomes of claudins, cadherins, and desmosomal proteins ( 156 , 157 , 158 , 159 ), suggesting that the upregulation or downregulation of at least some connexin members would have implications on the availability of binding proteins engaged in structural junction homeostasis. Recently, the second extracellular loop domain of Cx50 was found to be implicated in driving cell–cell adhesion through the regulated expression of adhesion molecules with direct implications in lens cell differentiation ( 160 , 161 ). GJs composed of Cx26 and/or Cx43 have been reported to establish adhesive sites at locations between radial fibers and neurons where they serve to guide neuronal migration ( 162 ).…”

Section: Noncanonical Roles Of Connexins and Connexins Fragmentsmentioning

confidence: 99%

Diversity in connexin biology

Lucaciu,

Leighton,

Hauser

et al. 2023

Journal of Biological Chemistry

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“…Cx50 is capable of mediating both homotypic interactions and heterotypic protein-protein or protein-membrane interactions as a cell adhesion molecule, all independent of its role in forming Cx channels [120,121]. Moreover, the second extracellular (E2) domain is identified as a critical domain for cell adhesion function, and the expression of Cx50 enhances the expression of the adhesive molecules N-cadherin and β-catenin [122]. Aquaporin 0 (AQP0), a water-channel-forming protein, is also reported to act as an adhesive molecule and is proposed to serve as a major structural protein to facilitate the formation of the ordered cellular structure in mature lens fibers [123].…”

Section: Cell Adhesionmentioning

confidence: 99%

Gap Junctions or Hemichannel-Dependent and Independent Roles of Connexins in Fibrosis, Epithelial–Mesenchymal Transitions, and Wound Healing

Li,

Acosta,

Jiang

2023

Biomolecules

Self Cite

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Fibrosis initially appears as a normal response to damage, where activated fibroblasts produce large amounts of the extracellular matrix (ECM) during the wound healing process to assist in the repair of injured tissue. However, the excessive accumulation of the ECM, unresolved by remodeling mechanisms, leads to organ dysfunction. Connexins, a family of transmembrane channel proteins, are widely recognized for their major roles in fibrosis, the epithelial–mesenchymal transition (EMT), and wound healing. Efforts have been made in recent years to identify novel mediators and targets for this regulation. Connexins form gap junctions and hemichannels, mediating communications between neighboring cells and inside and outside of cells, respectively. Recent evidence suggests that connexins, beyond forming channels, possess channel-independent functions in fibrosis, the EMT, and wound healing. One crucial channel-independent function is their role as the primary functional component for cell adhesion. Other channel-independent functions of connexins involve their roles in mitochondria and exosomes. This review summarizes the latest advances in the channel-dependent and independent roles of connexins in fibrosis, the EMT, and wound healing, with a particular focus on eye diseases, emphasizing their potential as novel, promising therapeutic targets.

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The second extracellular domain of connexin 50 is important for in cell adhesion, lens differentiation, and adhesion molecule expression

Cited by 4 publications

References 48 publications

Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics, and clarity

Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics, and clarity

Diversity in connexin biology

Gap Junctions or Hemichannel-Dependent and Independent Roles of Connexins in Fibrosis, Epithelial–Mesenchymal Transitions, and Wound Healing

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