1954
DOI: 10.1080/713818666
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The Second-Order Aberrations in the Interferometric Measurement of Concentration Gradients

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Cited by 14 publications
(5 citation statements)
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“…Unfortunately, when using commercially available cell components with 3-mm centerpieces, another problem arises: the optical system of the analytical ultracentrifuge is aligned such that the focus is in the 2/3 plane of the solution column of the standard 12-mm centerpieces. This minimizes the Wiener skewing from the refractive index gradients of the sedimenting sample (Forsberg and Svensson 1954 ; Svensson 1954 ) when using these standard centerpieces. However, this condition cannot be easily fulfilled with common cell components when using 3-mm centerpieces.…”
Section: Resultsmentioning
confidence: 99%
“…Unfortunately, when using commercially available cell components with 3-mm centerpieces, another problem arises: the optical system of the analytical ultracentrifuge is aligned such that the focus is in the 2/3 plane of the solution column of the standard 12-mm centerpieces. This minimizes the Wiener skewing from the refractive index gradients of the sedimenting sample (Forsberg and Svensson 1954 ; Svensson 1954 ) when using these standard centerpieces. However, this condition cannot be easily fulfilled with common cell components when using 3-mm centerpieces.…”
Section: Resultsmentioning
confidence: 99%
“…When working with highly concentrated solutions, as required for studying weak macromolecular interactions, 43,44 it is imperative with any optical detection system to use short optical path length centerpieces because optical aberrations from refractive index gradients increase with the third power of the path length. 45 In addition, the absorbance optical system has a limited linear range for which Lambert–Beer’s law is valid, and the Rayleigh optical interference system is limited in the strongest gradients that can be recorded without integral fringe offsets between neighboring radial points. 46 For equilibrium experiments, very short pathlengths have been achieved previously by utilizing gaskets made of thin films to serve as centerpiece, 47,48 but we found the loading process to be very problematic and were unable to create long solution columns suitable for SV with this approach.…”
Section: Resultsmentioning
confidence: 99%
“…When working with highly concentrated solutions, as required for studying weak macromolecular interactions, , it is imperative with any optical detection system to use short optical path length centerpieces because optical aberrations from refractive index gradients increase with the third power of the path length . In addition, the absorbance optical system has a limited linear range for which Lambert–Beer’s law is valid, and the Rayleigh optical interference system is limited in the strongest gradients that can be recorded without integral fringe offsets between neighboring radial points .…”
Section: Resultsmentioning
confidence: 99%
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“…The original light-absorption system (89,90) is finding favor again, for studying substances at low concentrations which absorb light strongfy and which show a marked dependence of sedimentation behavior on concentration. There are certain theoretical problems in obtaining a curve of dc/dr vs. r from a schlieren photograph (1,16,50,95) or in obtaining a curve of c vs. r from a photograph of Rayleigh fringes (27), but this subject will not be reviewed here. It will be assumed that n and dn/dr (or c and dc/dr when there is a single solute) are directly measurable quantities.…”
Section: Introductionmentioning
confidence: 99%