The secondary metabolites of rare actinomycetes: chemistry and bioactivity

Ding, Ting; Yang, Luo-Jie; Zhang, Weidong; Shen, Yun-Heng

doi:10.1039/c9ra03579f

Cited by 75 publications

(54 citation statements)

References 105 publications

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“…It is generally accepted that rare actinobacterial genera possess more potential in terms of secondary metabolites novelty ( Schorn et al, 2016 ; Bundale et al, 2019 ; Ding et al, 2019 ). Thus, exploration of bioactive chemicals from rare actinobacterial strains will be more promising and efficient.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu

Wang

et al. 2021

Front. Microbiol.

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Taklamakan desert is known as the largest dunefield in China and as the second largest shifting sand desert in the world. Although with long history and glorious culture, the Taklamakan desert remains largely unexplored and numerous microorganisms have not been harvested in culture or taxonomically identified yet. The main objective of this study is to explore the diversity, novelty, and pharmacological potential of the cultivable actinomycetes from soil samples at various sites along the Alar-Hotan desert highway in the Taklamakan desert. A total of 590 actinobacterial strains were recovered by the culture-dependent approach. Phylogenetic analysis based on 16S ribosomal RNA (rRNA) gene sequences unveiled a significant level of actinobacterial diversity with 55 genera distributed in 27 families of 12 orders. Thirty-six strains showed relatively low 16S rRNA similarities (<98.65%) with validly described species, among which four strains had already been characterized as novel taxa by our previous research. One hundred and forty-six actinobacterial isolates were selected as representatives to evaluate the antibacterial activities and mechanism of action by the paper-disk diffusion method and a double fluorescent protein reporter “pDualrep2” system, respectively. A total of 61 isolates exhibited antagonistic activity against the tested “ESKAPE” pathogens, among which seven strains could produce bioactive metabolites either to be able to block translation machinery or to induce SOS-response in the pDualrep2 system. Notably, Saccharothrix sp. 16Sb2-4, harboring a promising antibacterial potential with the mechanism of interfering with protein translation, was analyzed in detail to gain deeper insights into its bioactive metabolites. Through ultra-performance liquid chromatography (UPLC)-quadrupole time-of-flight (QToF)-MS/MS based molecular networking analysis and databases identification, four families of compounds (1–16) were putatively identified. Subsequent bioassay-guided separation resulted in purification of four 16-membered macrolide antibiotics, aldgamycin H (8), aldgamycin K (9), aldgamycin G (10), and swalpamycin B (11), and their structures were elucidated by HR-electrospray ionization source (ESI)-MS and NMR spectroscopy. All compounds 8–11 displayed antibacterial activities by inhibiting protein synthesis in the pDualrep2 system. In conclusion, this work demonstrates that Taklamakan desert is a potentially unique reservoir of versatile actinobacteria, which can be a promising source for discovery of novel species and diverse bioactive compounds.

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Section: Discussionmentioning

confidence: 99%

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu

Wang

et al. 2021

Front. Microbiol.

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“…Among those the predominant is actinomycetes, a group of saprophytic bacteria known to generate a broad range of secondary metabolites, bioactive compounds and antibiotics that control microbial growth. It is estimated that 70–80% of secondary metabolites and one-third of antibiotics available commercially till date have been obtained from actinomycetes ( Ding et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and antinematicidal metabolites from Streptomyces cuspidosporus strain SA4 against selected pathogenic bacteria, fungi and nematode

Sholkamy

Muthukrishnan²,

Abdel-Raouf

et al. 2020

Saudi Journal of Biological Sciences

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“…More attentions have been diverted to microorganisms that are previously inadequately assessed or from underexplored environments. The rare actinomycetes are referred to the actinomycetes strains less frequently obtained than that of commonly isolated Streptomyces spp., even though they might not actually be uncommon in the environment . Among rare actinomycete, the genera Micromonospora was observed to produce the highest numbers of natural products …”

Section: Introductionmentioning

confidence: 99%

Secondary Metabolites from Marine Micromonospora: Chemistry and Bioactivities

Gui

et al. 2020

Chemistry & Biodiversity

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Marine Micromonospora was revealed to be a rather untapped and a rich source of chemically diverse and unique bioactive natural products. This review is aimed to make a comprehensive survey of secondary metabolites that were derived from marine Micromonospora including chemical diversity and biological activities. A total of 116 compounds from 41 marine Micromonospora species have been reported, covering the literatures from 1997 to 2019. These compounds contain several structural classes such as polyketides (PKS), nonribosomal peptides (NRPS), PKS-NRPS hybrids, terpenes and others, and they present cytotoxic, antibacterial, antiparasitic, chemopreventive or antioxidant activities.

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The secondary metabolites of rare actinomycetes: chemistry and bioactivity

Abstract: A detailed introduction to the structures and bioactivities of secondary metabolites from rare actinomycetes is made.

Cited by 75 publications

References 105 publications

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Antimicrobial and antinematicidal metabolites from Streptomyces cuspidosporus strain SA4 against selected pathogenic bacteria, fungi and nematode

Secondary Metabolites from Marine Micromonospora: Chemistry and Bioactivities

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