The secondary metabolites produced by Lactobacillus plantarum downregulate BCL-2 and BUFFY genes on breast cancer cell line and model organism Drosophila melanogaster: molecular docking approach

Sentürk, Melih; Ercan, Fahriye Sumer; Yalçın, Serap

doi:10.1007/s00280-019-03978-0

Cited by 18 publications

(10 citation statements)

References 21 publications

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“…Additionally, upregulation of CASP3 gene expression in C26 cells was detected in cells being treated with L. plantarum , suggesting the activation of mitochondrial apoptotic pathways (Brentnall et al, 2013 ). Our data are consistent with the previous reports (Jiang et al, 2020 ; Sentürk et al, 2020 ; Sun et al, 2021 ; Yue et al, 2020 ).…”

Section: Discussionsupporting

confidence: 94%

The combined effect of fish oil containing Omega‐3 fatty acids and Lactobacillus plantarum on colorectal cancer

Sharifi

Yazdani

Najafi

et al. 2022

Food Science & Nutrition

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Colorectal cancer (CRC) is one of the deadliest malignancies. Recent attempts have indicated the role of diet in the etiology of CRC. Natural dietary compounds such as probiotics and Omega‐3 fatty acids that act synergistically can be beneficial in finding a tremendous solution against CRC. To date, the combined effect of fish oil containing Omega‐3 fatty acids (Omega‐3) and Lactobacillus plantarum (L. plantarum) on CRC has been left behind. We here evaluated the effects of co‐encapsulation of Omega‐3 and probiotic bacteria on CRC cell lines compared to normal cells. Omega‐3 and L. plantarum bacteria were co‐encapsulated in three ways, including gelatin–gum Arabic, gelatin–chitosan, and chitosan–gum Arabic complex coacervate microcapsules. After treatment of cells (Normal [L929] and colorectal [C26]) by L. plantarum, Omega‐3, and microcapsules, viability and growth capacity of cell lines were measured using the MTT (3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5‐diphenyltetrazolium bromide) assay. Isolated total RNA was used to evaluate the expression profile of BCL2‐associated X protein (BAX), B‐cell lymphoma 2 (BCL‐2), and Caspase‐3 (CASP3) genes by real‐time polymerase chain reaction (PCR). Statistical analysis was performed with SPSS 25 software. A value of p < .05 was considered statistically significant. The results indicated a significant reduction in cell viability of C26 in a concentration‐dependent manner in the treated cells with all treatments, except gelatin–gum Arabic microcapsules. The messenger RNA (mRNA) expression level of the BAX and CASP3 genes in C26 cells being treated with all treatments significantly increased than in untreated cells, and the expression level of the anti‐apoptotic factor of the BCL‐2 gene decreased in C26 cells simultaneously (p < .05). Although, the combined effect of Omega‐3 and L. plantarum and microcapsulated treatments had no more effect on viability and apoptosis gene expression of cancer cells compared to Omega‐3 or L. plantarum. In conclusion, combination therapy with fish oil containing Omega‐3 and L. plantarum does not improve the anticancer effect of each alone.

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Section: Discussionsupporting

confidence: 94%

The combined effect of fish oil containing Omega‐3 fatty acids and Lactobacillus plantarum on colorectal cancer

Sharifi

Yazdani

Najafi

et al. 2022

Food Science & Nutrition

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“…Another study reported that an exopolysaccharide isolated from the probiotic L. plantarum RJF4 showed no toxicity to the rat myoblast L6 cell line and have been proposed to use this polysaccharide as a food additive or for therapeutical applications [29]. Sentürk, Ercan and Yalcin [30] reported an IC 50 value of the secondary metabolite produced by L. plantarum, isolated from animal sources, in the MCF-7 cell line of 0.0011 mg mL −1 .…”

Section: Cytotoxicity and Inflammatory Activities Of Cfs Fractionsmentioning

confidence: 99%

Antimicrobial activity of protein-containing fractions isolated from Lactobacillus plantarum NRRL B-4496 culture

et al. 2020

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The interest in lactic acid bacteria, including Lactobacillus plantarum NRRL B-4496, has increased in recent years as biopreservatives, due to the production of secondary metabolites capable of inhibiting pathogenic bacteria. The objectives of this study were to evaluate the antimicrobial activity, cytotoxicity and the anti-inflammatory response of L. plantarum NRRL B-4496 cell-free supernatant (CFS). Furthermore, the CFS was fractionated by size exclusion chromatography using Sephadex G-25, and a minimal inhibitory volume test was determined against a panel of pathogenic bacteria. The cytotoxicity and the inflammatory activities of the fractions were evaluated using the human-derived THP-1 cell line. Results of this study indicates that CFS of L. plantarum NRRL B-4496 possesses antimicrobial protein compounds against the pathogen Listeria monocytogenes and showed no toxicity nor a pro-inflammatory response to human macrophages. The obtained results contribute to the development of novel bio-preservatives, L. plantarum cell-free supernatant or its fractions, with a potential use in the food industry.

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“…Molecular docking was carried out to determine the direct interaction of selected probiotic secretory molecules (bacteriocins) with key CRC-associated proteins. The molecular docking method we applied in this study was used to replicate a few protein-ligand binding affinity scores of the previously published computational studies to get confidence in the method (Anwar et al, 2020 ; Sentürk et al, 2020 ). The 3D structures of the ligands (probiotic-derived bacteriocins) in “.sdf” format were obtained from PubChem ( https://pubchem.ncbi.nlm.nih.gov/ ).…”

Section: Methodsmentioning

confidence: 99%

Effects of Probiotics at the Interface of Metabolism and Immunity to Prevent Colorectal Cancer-Associated Gut Inflammation: A Systematic Network and Meta-Analysis With Molecular Docking Studies

et al. 2022

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BackgroundDysbiosis/imbalance in the gut microbial composition triggers chronic inflammation and promotes colorectal cancer (CRC). Modulation of the gut microbiome by the administration of probiotics is a promising strategy to reduce carcinogenic inflammation. However, the mechanism remains unclear.MethodsIn this study, we presented a systematic network, meta-analysis, and molecular docking studies to determine the plausible mechanism of probiotic intervention in diminishing CRC-causing inflammations.ResultsWe selected 77 clinical, preclinical, in vitro, and in vivo articles (PRISMA guidelines) and identified 36 probiotics and 135 training genes connected to patients with CRC with probiotic application. The meta-analysis rationalizes the application of probiotics in the prevention and treatment of CRC. An association network is generated with 540 nodes and 1,423 edges. MCODE cluster analysis identifies 43 densely interconnected modules from the network. Gene ontology (GO) and pathway enrichment analysis of the top scoring and functionally significant modules reveal stress-induced metabolic pathways (JNK, MAPK), immunomodulatory pathways, intrinsic apoptotic pathways, and autophagy as contributors for CRC where probiotics could offer major benefits. Based on the enrichment analyses, 23 CRC-associated proteins and 7 probiotic-derived bacteriocins were selected for molecular docking studies. Results indicate that the key CRC-associated proteins (e.g., COX-2, CASP9, PI3K, and IL18R) significantly interact with the probiotic-derived bacteriocins (e.g., plantaricin JLA-9, lactococcin A, and lactococcin mmfii). Finally, a model for probiotic intervention to reduce CRC-associated inflammation has been proposed.ConclusionProbiotics and/or probiotic-derived bacteriocins could directly interact with CRC-promoting COX2. They could modulate inflammatory NLRP3 and NFkB pathways to reduce CRC-associated inflammation. Probiotics could also activate autophagy and apoptosis by regulating PI3K/AKT and caspase pathways in CRC. In summary, the potential mechanisms of probiotic-mediated CRC prevention include multiple signaling cascades, yet pathways related to metabolism and immunity are the crucial ones.

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The secondary metabolites produced by Lactobacillus plantarum downregulate BCL-2 and BUFFY genes on breast cancer cell line and model organism Drosophila melanogaster: molecular docking approach

Cited by 18 publications

References 21 publications

The combined effect of fish oil containing Omega‐3 fatty acids and Lactobacillus plantarum on colorectal cancer

The combined effect of fish oil containing Omega‐3 fatty acids and Lactobacillus plantarum on colorectal cancer

Antimicrobial activity of protein-containing fractions isolated from Lactobacillus plantarum NRRL B-4496 culture

Effects of Probiotics at the Interface of Metabolism and Immunity to Prevent Colorectal Cancer-Associated Gut Inflammation: A Systematic Network and Meta-Analysis With Molecular Docking Studies

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