The secretion and metabolism of cumulus cells support fertilization in the bovine model

Kato, Yoku; Ooshima, Yuki; Sasaki, Aiko; Yoshikawa, Eri; Xu, Hui; Nagao, Yoshikazu

doi:10.1016/j.theriogenology.2022.08.025

Cited by 2 publications

(1 citation statement)

References 53 publications

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“…Our findings are in agreement with a study reporting a decline in the percentage of A3-positive sperm (an indicator for abnormal chromatin packaging) in COCpenetrated spermatozoa compared with control spermatozoa [24]. This is not surprising, since there is evidence that cumulus cells undergo metabolic events to reduce oxidative stress in fertilization media [6,40].…”

Section: Discussionsupporting

confidence: 93%

Exposure to Cumulus Cell Secretome Improves Sperm Function: New Perspectives for Sperm Selection In Vitro

Luongo,

Perez Casasus,

Haxhiu

et al. 2023

Cells

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In the literature, there is a well-known correlation between poor semen quality and DNA sperm integrity, which can turn into negative outcomes in terms of embryo development and clinical pregnancy. Sperm selection plays a pivotal role in clinical practice, and the most widely used methods are mainly based on sperm motility and morphology. The cumulus oophorus complex (COC) during natural fertilization represents a barrier that spermatozoa must overcome to reach the zona pellucida and fertilize the oocyte. Spermatozoa that can pass through the COC have better structural and metabolic characteristics as well as enhanced acrosome reaction (AR). The present study aimed to evaluate the exposure of sperm to cumulus cell secretome during swim-up treatment (SUC) compared with the routinely used swim-up method (SU). To determine the effectiveness of this method, biological factors critical for the ability of sperm to fertilize an oocyte, including capacitation, AR, tyrosine phosphorylation signature, DNA integrity, and mitochondrial functionality, were assessed. The SUC selection assures recovery of high-quality spermatozoa, with enhanced mitochondrial functionality and motility compared with both SU-selected and unselected (U) sperm. Furthermore, using this modified swim-up procedure, significantly reduced sperm DNA damage (p < 0.05) was detected. In conclusion, the SUC approach is a more physiological and integrated method for sperm selection that deserves further investigation for its translation into clinical practice.

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Section: Discussionsupporting

confidence: 93%

Exposure to Cumulus Cell Secretome Improves Sperm Function: New Perspectives for Sperm Selection In Vitro

Luongo,

Perez Casasus,

Haxhiu

et al. 2023

Cells

View full text Add to dashboard Cite

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Exposure to Cumulus Cell Secretome Improves Sperm Function: New Perspectives for Sperm Selection <em>In Vitro</em>

Luongo,

Perez Casasus,

Haxhiu

et al. 2023

Preprint

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In the literature, it is well known the correlation between poor semen quality and DNA sperm integrity, which can turn into negative outcomes in terms of embryo development and clinical pregnancy. Sperm selection plays a pivotal role in clinical practice and the most widely used methods are mainly based on sperm motility and morphology. The cumulus oophorus complex (COC) during natural fertilization represents a barrier that spermatozoa must overcome to reach the zona pellucida and fertilize the oocyte. Spermatozoa that can pass through the COC have better structural and metabolic characteristics as well as enhanced acrosome reaction (AR). The present study aimed to evaluate the exposure of sperm to cumulus cell secretome during swim-up treatment (SUC), compared to the routinely used swim-up method (SU). To determine the effectiveness of this method biological factors critical for the ability of sperm to fertilize an oocyte, including capacitation, AR, tyrosine phosphorylation signature, DNA integrity, and mitochondrial functionality were assessed. The SUC selection assures recovery of high-quality spermatozoa, with enhanced mitochondrial functionality and motility compared to both SU-selected or unselected (U) sperm. Furthermore, by this modified swim-up procedure significantly reduced sperm DNA damage (p<0.05) was detected. In conclusion, the SUC approach is a more physiological and integrated method for sperm selection that deserves further investigation for its translation into clinical practice.

show abstract

The secretion and metabolism of cumulus cells support fertilization in the bovine model

Cited by 2 publications

References 53 publications

Exposure to Cumulus Cell Secretome Improves Sperm Function: New Perspectives for Sperm Selection In Vitro

Exposure to Cumulus Cell Secretome Improves Sperm Function: New Perspectives for Sperm Selection In Vitro

Exposure to Cumulus Cell Secretome Improves Sperm Function: New Perspectives for Sperm Selection <em>In Vitro</em>

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