The Secretion of N-Acetylglucosaminidase during Germ-tube Formation in Candida albicans

Sullivan, Patrick A.; McHugh, Nicola; Romana, Lajwant K.; Shepherd, Maxwell G.

doi:10.1099/00221287-130-9-2213

Cited by 31 publications

(31 citation statements)

References 7 publications

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“…These enzymes may also be involved in the process of tinea development since they can metabolize a series of substrates including different human skin components. Similar results were obtained for T. rubrum (8), M. canis (23) and Candida albicans (29).…”

Section: T Rubrumsupporting

confidence: 75%

18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES

Nascimento¹,

Martinez-Rossi²

2001

Braz. J. Microbiol.

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Dermatophytes, capable to use keratin of the host for nutrition, belong to one of the major groups of pathogenic fungi. Since dermatophytes are a closely related group they share various common features, and the morphology of isolates of a given species can be atypical, making species identification and differentiation even more difficult. Many methods have been explored in attempts to distinguish dermatophytes, but the combined use of different approaches for the investigation of the intraspecific and interspecific variability of Trichophyton continues to be scarce. Some studies have shown that amplified fragments of the small ribosomal DNA subunit 18S contains variable regions which can be used to discriminate between medically relevant yeast species, indicating that these regions could also be used for differentiation between dermatophytes. In our study, sequence analysis of the 18S-rDNA gene was combined with morphological and biochemical criteria in order to detect genetic differences between seven Trichophyton isolates and estimate their phylogenetic relationships. The results show that the isolates investigated belong to the Trichophyton group, which potentially contains the Trichophyton rubrum cluster.

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Section: T Rubrumsupporting

confidence: 75%

18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES

Nascimento¹,

Martinez-Rossi²

2001

Braz. J. Microbiol.

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“…Both trehalase and glucoamylase are induced by ethanol in Saccharomyces cerevisiae (Panek, 1962;Rambeck & Simon, 1972) and mobilization of glycogen does seem to correlate with germ tube formation in C. albicans . Wall lytic enzymes may also be required for germ tube formation (Ram et al, 1984;Sullivan et al, 1984). Recent reports on proteases secreted by C. albicans (MacDonald, 1984;Samaranayake et al, 1984;Ruchel, 1984) have all emphasized their possible roles in the pathogenicity of C. albicans, but have not considered the possibility that the breakdown of cytoplasmic materials or wall glycoproteins may also be integrally involved in germ tube formation.…”

Section: J H P O L L a C K A N D T Hashimoto Discussionmentioning

confidence: 99%

“…These changes range from the production of rhizomorphs, instead of mycelia, in Armillaria mellea (Weinhold, 1963), germination of macrospores of Fusarium solani (Cochrane et al, 1963;Paz et al, 1984), formation of Saccharomyces cerevisiae ascospores (Miller & Halpern,! 956), branching of germ tubes of Peronospora parasitica (McMeekin, 1981) and germ tube formation by Aureobasidium pullulans (Sevilla et al, 1983) and Candida tropicalis (Tani et a/., 1979). There has been only one previous report of Candida albicans forming germ tubes when the growth medium was supplemented with ethanol (Reynouard et al, 1979).…”

Section: Introductionmentioning

confidence: 96%

Ethanol-induced Germ Tube Formation in Candida albicans

Pollack¹,

Hashimoto²

1985

Microbiology

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“…With MU-[G1cNAc]n (n>2), exochitinase would also release (G1cNAc)2 units. Sullivan et al purified an enzyme from C. albicans which was named Nacetylglucosaminidase (21). This enzyme was active against PNP-G1cNAc,…”

Section: Discussionmentioning

confidence: 99%

Discrimination between chitinase and chitobiase activities in Candida albicans.

Overdijk¹,

Steijn²

1995

J. Gen. Appl. Microbiol.

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Measurement of chitinase activity in crude homogenates of Candida albicans with the artificial substrates 4-methylumbelliferyl /3-D-N,N',N"-triacetylchitotrioside and 4-methylumbelliferyl/3-D-N,N'-diacetylchitobioside (MU-[G1cNAc]3,2) showed that both chitobiase and chitinase were involved in the liberation of the fluorophore methylumbelliferone (MU). The substrate MU-[G1cNAc]3 appeared to be digested by means of two separate mechanisms, resulting in a lag-phase in the time curve of the MU-release, and thus making this release non-linear with time. The first mechanism is the action of an endochitinase that released MU in one step; this reaction could be inhibited by allosamidin. The second probable mechanism involves the digestion of the substrate by the stepwise release of the outer two G1cNAc residues by a chitobiase, followed either by a third chitobiase-catalyzed release of the final G1cNAc or by the action of a,(3-N-acetylhexosaminidase, that is also present in C. albicans. Hydrolysis of the substrate MU-[ G1cNAc ] 2 was not inhibited by allosamidin, suggesting that the two G1cNAc residues in this substrate are probably released in two steps, either by the action of chitobiase alone, or by the successive action of chitobiase (step 1) and /3-N-acetylhexosaminidase (step 2). Our results show that the activities of the individual enzymes involved in chitin degradation cannot be determined on the basis of MU release in unfractionated homogenates of C. albicans.The literature on chitinases has been reviewed recently by Flach et al. (7).

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The Secretion of N-Acetylglucosaminidase during Germ-tube Formation in Candida albicans

Cited by 31 publications

References 7 publications

18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES

18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES

Ethanol-induced Germ Tube Formation in Candida albicans

Discrimination between chitinase and chitobiase activities in Candida albicans.

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