The Secretive Liaison of Particulate Matter and SARS-CoV-2. A Hypothesis and Theory Investigation

Mescoli, Ada; Maffei, Giangabriele; Pillo, Gelsomina; Bortone, Giuseppe; Marchesi, Stefano; Morandi, Elena; Ranzi, Andrea; Rotondo, Francesca; Serra, Stefania; Vaccari, Monica; Zauli-Sajani, Stefano; Mascolo, Maria Grazia; Jacobs, Miriam N.; Colacci, Annamaria

doi:10.3389/fgene.2020.579964

Cited by 16 publications

(15 citation statements)

References 113 publications

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“…It is postulated that the first steps in the inflammation signaling could be: mitogen activated kinases (MAPKs) activity and Pattern Recognition Receptors (PPRs) signaling activation [ 45 ]. PPRs have been reported to recognize both pathogens (PAMPs, Pathogen-Associated Molecular Patterns) and endogenous ligands released by distressed or damaged cells, generally called DAMPs (Damage-Associated Molecular Patterns) [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Mechanistic Interrogation of Cell Transformation In Vitro: The Transformics Assay as an Exemplar of Oncotransformation

Pillo

Mascolo

Zanzi

et al. 2022

IJMS

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The Transformics Assay is an in vitro test which combines the BALB/c 3T3 Cell Transformation Assay (CTA) with microarray transcriptomics. It has been shown to improve upon the mechanistic understanding of the CTA, helping to identify mechanisms of action leading to chemical-induced transformation thanks to RNA extractions in specific time points along the process of in vitro transformation. In this study, the lowest transforming concentration of the carcinogenic benzo(a)pyrene (B(a)P) has been tested in order to find molecular signatures of initial events relevant for oncotransformation. Application of Enrichment Analysis (Metacore) to the analyses of the results facilitated key biological interpretations. After 72 h of exposure, as a consequence of the molecular initiating event of aryl hydrocarbon receptor (AhR) activation, there is a cascade of cellular events and microenvironment modification, and the immune and inflammatory responses are the main processes involved in cell response. Furthermore, pathways and processes related to cell cycle regulation, cytoskeletal adhesion and remodeling processes, cell differentiation and transformation were observed.

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Section: Discussionmentioning

confidence: 99%

Mechanistic Interrogation of Cell Transformation In Vitro: The Transformics Assay as an Exemplar of Oncotransformation

Pillo

Mascolo

Zanzi

et al. 2022

IJMS

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“…It is postulated that the rst steps in the in ammation loop signaling could be: mitogen activated kinases (MAPKs) activity and pattern recognition receptors (PPRs) signaling activation 31 .…”

Section: Discussionmentioning

confidence: 99%

Mechanistic Interrogation of Cell Transformation in Vitro: The Transformics Assay as an Exemplar of Oncotransformation

Pillo

Mascolo

Zanzi

et al. 2021

Preprint

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The rodent-based life-time cancer bioassay is still the basic experimental paradigm to assess the carcinogenic potential of chemicals, with relatively little progress regarding the acceptance of in vitro tests. The Transformics Assay is an in vitro test which combines the BALB/c 3T3 Cell Transformation Assay (CTA) with microarray transcriptomics. It has been shown to improve upon the mechanistic understanding of the CTA, helping to identify mechanisms of action leading to chemical-induced transformation thanks to RNA extractions in specific time points along the process of in vitro transformation. In this study the lowest transforming concentration of the carcinogenic benzo(a)pyrene (B(a)P) has been tested in order to find molecular signatures of initial events relevant for oncotransformation. Some biological interpretations are presented related to the Enrichment Analysis provided by Metacore. Results show that after 72 hrs of exposure, the immune and inflammatory responses are the main processes involved in cell response. Furthermore, pathways and processes related to cell cycle regulation, cytoskeletal adhesion and remodeling processes, cell differentiation and transformation were observed. All these data are interpreted as a consequence of the initial aryl hydrocarbon receptor (AhR) activation leading to a cascade of cellular events and microenvironment modification.

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“…PM can be exacerbated by the inflammation induced by COVID-19. Therefore, PM may play a role as a booster of COVID-19 ( Mescoli et al, 2020 ). It is assumed that PM could be the transmission model of SARS-CoV-2 infection and could be the “carrier” of SARS-CoV-2, which enters the human body directly ( Tung et al, 2021 ).…”

Section: Overview Of Possible Transmission Pathwaysmentioning

confidence: 99%

The role of airborne particles and environmental considerations in the transmission of SARS-CoV-2

Shao¹,

Ge²,

Jones³

et al. 2021

Geoscience Frontiers

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The Secretive Liaison of Particulate Matter and SARS-CoV-2. A Hypothesis and Theory Investigation

Cited by 16 publications

References 113 publications

Mechanistic Interrogation of Cell Transformation In Vitro: The Transformics Assay as an Exemplar of Oncotransformation

Mechanistic Interrogation of Cell Transformation In Vitro: The Transformics Assay as an Exemplar of Oncotransformation

Mechanistic Interrogation of Cell Transformation in Vitro: The Transformics Assay as an Exemplar of Oncotransformation

The role of airborne particles and environmental considerations in the transmission of SARS-CoV-2

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