The selective role of ECM components on cell adhesion, morphology, proliferation and communication in vitro

Schlie-Wolter, Sabrina; Ngezahayo, Anaclet; Chichkov, Boris N.

doi:10.1016/j.yexcr.2013.03.016

Cited by 111 publications

(102 citation statements)

References 41 publications

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“…Neuroblastoma cells responded best to LA. A cell-specific ligand priority ranking was confirmed elsewhere [4]. This finding might be the first key to explain the topographical control of fibroblasts versus neuroblastoma cells: it can be supposed that the surface texturing negatively affects the adsorption of the fibroblast-specific adhesion ligand FIB, while the adsorption of the neuroblastoma-specific adhesion ligand LA is not negatively affected.…”

supporting

confidence: 54%

“…This environment in vivo is actually pretty well defined. Cells are always embedded in a tissue-specific extracellular matrix (ECM), which basically consists of soluble molecules, glycosaminoglycans, proteoglycans and adhesive proteins to anchorage cells [3,4]. Besides the structural support, cell binding to the ECM stimulates outside-in signalling cascades needed for survival, cell cycle progression and differentiation [5].…”

Section: Introductionmentioning

confidence: 99%

“…On the one hand, this process stabilizes cell-binding to the surface via the additional recruitment of other cytoskeletal elements [36,37]. On the other hand, the now-formed focal adhesion complex initiates outside-in signalling cascades, which depend on many different components [4,38]. These interactions have an impact on cell migration but also stimulate transcription factors within the nucleus, which then control survival, proliferation, intercellular communication and differentiation.…”

mentioning

confidence: 99%

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Impact of laser-structured biomaterial interfaces on guided cell responses

et al. 2014

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To achieve a perfect integration of biomaterials into the body, tissue formation in contact with the interface has to be controlled. In this connection, a selective cell control is required: fibrotic encapsulation has to be inhibited, while tissue guidance has to be stimulated. As conventional biomaterials do not fulfil this specification, functionalization of the biointerface is under development to mimic the natural environment of the cells. One approach focuses on the fabrication of defined surface topographies. Thereby, ultrashort pulse laser ablation is very beneficial, owing to a large variety of fabricated structures, reduced heat-affected zones, high precision and reproducibility. We demonstrate that nanostructures in platinum and microstructures in silicon selectively control cell behaviour: inhibiting fibroblasts, while stimulating neuronal attachment and differentiation. However, the control of fibroblasts strongly correlates with the created size dimensions of the surface structures. These findings suggest favourable biomaterial interfaces for electronic devices. The mechanisms which are responsible for selective cell control are poorly understood. To give an insight, cell behaviour in dependence of biomaterial interfaces is discussed—including basic research on the role of the extracellular matrix. This knowledge is essential to understand such specific cell responses and to optimize biomaterial interfaces for future biomedical applications.

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confidence: 54%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Impact of laser-structured biomaterial interfaces on guided cell responses

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“…It is established that the assembly/maturation of focal adhesion involves the translocation and concentration of a heterogeneous group of at least 50 different molecules including cytoskeleton proteins and proteoglycans (vinculin, talin, actin, tensin, syndecans, etc.) at the adherent junctions in the plasma membrane [34]. The presence and function of heparan sulfate proteoglycans such as syndercan-4 at these focal adhesion complexes is critical to complete the focal adhesion assembly process [35].…”

Section: Discussionmentioning

confidence: 99%

Fetuin-A associates with histones intracellularly and shuttles them to exosomes to promote focal adhesion assembly resulting in rapid adhesion and spreading in breast carcinoma cells

Nangami

Koumangoye

Goodwin

et al. 2014

Experimental Cell Research

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The present analyses were undertaken to define the mechanisms by which fetuin-A modulates cellular adhesion. FLAG-tagged fetuin-A was expressed in breast carcinoma and HEK-293T cells. We demonstrated by confocal microscopy that fetuin-A co-localizes with histone H2A in the cell nucleus, forms stable complexes with histones such as H2A and H3 in solution, and shuttles histones to exosomes. The rate of cellular adhesion and spreading to either fibronectin or laminin coated wells was accelerated significantly in the presence of either endogenous fetuin-A or serum derived protein. More importantly, the formation of focal adhesion complexes on surfaces coated by laminin or fibronectin was accelerated in the presence of fetuin-A or histone coated exosomes. Cellular adhesion mediated by histone coated exosomes was abrogated by heparin and heparinase III. Heparinase III cleaves heparan sulfate from cell surface heparan sulfate proteoglycans. Lastly, the uptake of histone coated exosomes and subsequent cellular adhesion, was abrogated by heparin. Taken together, the data suggest a mechanism where fetuin-A, either endogenously synthesized or supplied extracellularly can extract histones from the nucleus or elsewhere in the cytosol/membrane and load them on cellular exosomes which then mediate adhesion by interacting with cell surface heparan sulfate proteoglycans via bound histones.

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“…Bone matrix, for instance, contains 90% of collagen type I and only 5% of noncollagenous proteins like osteocalcin, osteonectin, fibronectin, or hyaluronan and mineral compounds which are essential to conserve osteoblasts phenotype [55], whereas culturing chondrocytes on type I collagen induces their dedifferentiation [56]. Furthermore ECM components selectively influence cell adhesion and shape as described by Schlie-Wolter et al [57]. Hence, cell morphology directed by the interaction with ECM induces modifications of their behaviour and subsequently their fate [58].…”

Section: Microenvironmental Effects Of Extracellular Matrixmentioning

confidence: 99%

Cell Microenvironment Engineering and Monitoring for Tissue Engineering and Regenerative Medicine: The Recent Advances

Barthès

Özçelik

Hindié

et al. 2014

BioMed Research International

194

129

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In tissue engineering and regenerative medicine, the conditions in the immediate vicinity of the cells have a direct effect on cells' behaviour and subsequently on clinical outcomes. Physical, chemical, and biological control of cell microenvironment are of crucial importance for the ability to direct and control cell behaviour in 3-dimensional tissue engineering scaffolds spatially and temporally. In this review, we will focus on the different aspects of cell microenvironment such as surface micro-, nanotopography, extracellular matrix composition and distribution, controlled release of soluble factors, and mechanical stress/strain conditions and how these aspects and their interactions can be used to achieve a higher degree of control over cellular activities. The effect of these parameters on the cellular behaviour within tissue engineering context is discussed and how these parameters are used to develop engineered tissues is elaborated. Also, recent techniques developed for the monitoring of the cell microenvironment in vitro and in vivo are reviewed, together with recent tissue engineering applications where the control of cell microenvironment has been exploited. Cell microenvironment engineering and monitoring are crucial parts of tissue engineering efforts and systems which utilize different components of the cell microenvironment simultaneously can provide more functional engineered tissues in the near future.

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The selective role of ECM components on cell adhesion, morphology, proliferation and communication in vitro

Cited by 111 publications

References 41 publications

Impact of laser-structured biomaterial interfaces on guided cell responses

Impact of laser-structured biomaterial interfaces on guided cell responses

Fetuin-A associates with histones intracellularly and shuttles them to exosomes to promote focal adhesion assembly resulting in rapid adhesion and spreading in breast carcinoma cells

Cell Microenvironment Engineering and Monitoring for Tissue Engineering and Regenerative Medicine: The Recent Advances

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